Biosensing approach for alcohol determination using immobilized alcohol oxidase

Date

2006-06-15

Author

Yildiz, Huseyin Bekir
Toppare, Levent Kamil

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

154
views

0
downloads

Alcohol oxidase (AOD) was immobilized in polypyrrole (PPy) and a random copolymer containing 3-methylthienyl methacrylate and p-vinylbenzyloxy poly(ethyleneoxide) matrices. Immobilization of enzyme was performed via entrapment in conducting polymers during electrochemical polymerization of pyrrole through the thiophene moiety of the copolymer. Three different alcohols, namely methanol, ethanol and n-propanol, were used as substrates. Maximum reaction rates, Michaelis-Menten constants, optimum temperature and pH values, operational stabilities and shelf life of the enzyme electrodes were investigated.

Subject Keywords

Biotechnology, Biophysics, Electrochemistry, Biomedical Engineering, General Medicine

URI

https://hdl.handle.net/11511/37239

Journal

BIOSENSORS & BIOELECTRONICS

DOI

https://doi.org/10.1016/j.bios.2005.11.006

Collections

Department of Chemistry, Article

Suggestions

OpenMETU
Core

Syntheses of electroactive layers based on functionalized anthracene for electrochromic applications Yıldırım, Ahmet; Tarkuc, S.; AK, MESUT; Toppare, Levent Kamil (Elsevier BV, 2008-05-30) A new monomer (DTAT) was synthesized via linking 3,4-ethylenedioxythiophene (EDOT) on anthracene. The polymer, P(DTAT) was electrosynthesized by anodic oxidation of the corresponding monomer in 0.1 M LiClO4 acetonitrile (ACN) solution. The optical properties, the absorption spectra and the kinetics, were examined. Spectroelectrochemical analysis showed that P(DTAT) has an electronic band gap (due to pi-pi* transition) of 1.57 eV at 776 nm.
Anodic polymerization of 2,5-di-(2-thienyl)-furan in ethanol Icli, Merve; Cihaner, Atilla; Oenal, Ahmet A. (Elsevier BV, 2007-11-01) Poly(2,5-di-(2-thienyl)-furan) (PSOS) was synthesized via anodic polymerization of 2,5-di-(2-thienyl)-furan (SOS) in ethanol solution containing 0.2 M LiClO4 as supporting electrolyte. The electrochemical and spectroelectrochemical properties were investigated using electroanalytical and UV-vis spectroscopic techniques, respectively. The band gap of the polymer film was found as 2.22 eV and the film was successfully switched between black oxidized state and orange neutral state. Fluorescence and electrochem...
Resorbable elastomeric networks prepared by photocrosslinking of high-molecular-weight poly(trimethylene carbonate) with photoinitiators and poly(trimethylene carbonate) macromers as crosslinking aids Bat, Erhan; Feijen, Jan; Grijpma, Dirk W. (Elsevier BV, 2011-05-01) Resorbable and elastomeric poly( trimethylene carbonate) (PTMC) networks were efficiently prepared by photoinitiated crosslinking of linear high-molecular-weight PTMC To crosslink PTMC films, low-molecular-weight PTMC macromers with methacrylate end groups were synthesized and used as crosslinking aids By exposing PTMC films containing only photoinitiator (Irgacure (R) 2959) or both photoinitiator and PTMC macromers to ultraviolet light. PTMC networks with high gel contents (87-95%) could be obtained The cr...
Spectroelectrochemical investigation of the anodic oxidation of dibenzo-18-crown-6 Cihaner, A; Önal, Ahmet Muhtar (Elsevier BV, 2004-10-01) Poly(dibenzo-18-crown-6) (poly-DB18C6) was synthesized by electrochemical oxidation of dibenzo-18-crown-6 (DB18C6) using a mixture of acetonitrile and dichloromethane as the solvent and tetrabutylammonium tetrafluoroborate (TBABF(4)) or tetrabutylammonium hexafluorophosphate (TBAPF(6)) as supporting electrolyte. The anodic polymerization of DB18C6 was investigated using in situ ESR and in situ UV-Vis spectroscopic techniques. Spectroelectrochemical (SPEL), properties and thermal analysis of the resulting po...
Microstructure, microhardness, and biocompatibility characteristics of yttrium hydroxyapatite doped with fluoride Toker, S. M.; Tezcaner, Ayşen; Evis, Zafer (Wiley, 2011-02-01) The current study focused on doping of hydroxyapatite (HA) with constant yttrium (Y3+) and varying fluoride (F-) compositions to investigate its microstructure, microhardness, and biocompatibility. HA was synthesized by precipitation method and sintered at 1100 degrees C for 1 h. Y3+ and F- ion dopings resulted in changes in densities. In x-ray diffraction analysis, no secondary phase formation was observed. Lattice parameters decreased upon ion substitutions. Scanning electron microscopy (SEM) results show...

Citation Formats

H. B. Yildiz and L. K. Toppare, “Biosensing approach for alcohol determination using immobilized alcohol oxidase,” BIOSENSORS & BIOELECTRONICS, pp. 2306–2310, 2006, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/37239.