Biosensing approach for alcohol determination using immobilized alcohol oxidase

Date

2006-06-15

Author

Yildiz, Huseyin Bekir
Toppare, Levent Kamil

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Alcohol oxidase (AOD) was immobilized in polypyrrole (PPy) and a random copolymer containing 3-methylthienyl methacrylate and p-vinylbenzyloxy poly(ethyleneoxide) matrices. Immobilization of enzyme was performed via entrapment in conducting polymers during electrochemical polymerization of pyrrole through the thiophene moiety of the copolymer. Three different alcohols, namely methanol, ethanol and n-propanol, were used as substrates. Maximum reaction rates, Michaelis-Menten constants, optimum temperature and pH values, operational stabilities and shelf life of the enzyme electrodes were investigated.

Subject Keywords

Biotechnology, Biophysics, Electrochemistry, Biomedical Engineering, General Medicine

URI

https://hdl.handle.net/11511/37239

Journal

BIOSENSORS & BIOELECTRONICS

DOI

https://doi.org/10.1016/j.bios.2005.11.006

Collections

Department of Chemistry, Article

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Citation Formats

H. B. Yildiz and L. K. Toppare, “Biosensing approach for alcohol determination using immobilized alcohol oxidase,” BIOSENSORS & BIOELECTRONICS, pp. 2306–2310, 2006, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/37239.