Diesel Oil Degradation Potential of a Bacterium Inhabiting Petroleum Hydrocarbon Contaminated Surface Waters and Characterization of Its Emulsification Ability

Onur, Gozde
Yılmaz, Fadime
İçgen, Bülent
Degradation of poorly water soluble hydrocarbons, like n-alkanes and polycyclic aromatic hydrocarbons are challenged by some bacteria through emulsification of hydrocarbons by producing biosurfactants. In diesel oil bioremediation, diesel oil degrading and surfactant producing bacteria are used to eliminate these pollutants from contaminated waters. Therefore, identifying and characterizing bacteria capable of producing surfactant and degrading diesel oil are pivotal. In this study, bacteria isolated from hydrocarbon contaminated river water were screened for their potential to degrade diesel oil. Primary selection was carried out by using conventional enrichment culture technique, emulsification index measurement, gravimetric and gas chromatographic analyses of diesel oil degradation. A bacterium with 60 % emulsification index and 92 % diesel oil degradation ability in 14 days was identified as Acinetobacter haemolyticus Zn01 by 16S rRNA sequencing. A. haemolyticus Zn01 was shown to harbor both catabolic genes alkB and C23O effective in diesel oil degradation. The biosurfactant of the bacterium was also characterized in terms of surface tension, zeta potential, Fourier transform infrared spectroscopy and scanning electron microscopy. Being able to emulsify and degrade diesel oil, A. haemolyticus Zn01 seems to have high potential for the elimination of diesel oil from polluted waters.


Cytochrome P4501A and associated mixed-function oxidase induction in fish as a biomarker for toxic carcinogenic pollutants in the aquatic environment
Arinc, E; Sen, A; Bozcaarmutlu, A (Walter de Gruyter GmbH, 2000-06-01)
Polycyclic aromatic hydrocarbons (PAHs), dioxins, dibenzofurans, and polychlorinated biphenyls (PCBs) present in polluted environment induce cytochrome P4501A (CYP1A) isozyme in fish, which in turn results in a marked increased production of carcinogenic metabolites from PAHs. The induction of hepatic CYP1A in fish by certain classes of chemicals has been suggested as an early warning system, a "most sensitive biological response" for assessing environmental contamination conditions. This has implications f...
Optimization of supercritical CO2 extraction of Thymbra spicata oil
Sonsuzer, S; Şahin, Serpil; Yılmaz, Levent (Elsevier BV, 2004-07-01)
The supercritical fluid extraction (SFE) of aroma compounds from Thymbra spicata was studied. Response surface methodology (RSM) was used to optimize the parameters of the supercritical fluid extraction. Independent variables were temperature (40, 50 and 60 degreesC), pressure (80, 100 and 120 bar) and time (30, 60 and 90 min). Dependent variables were yield, monoterpene, sesquiterpene and oxygenated monoterpene contents.
Mn-Cu and Mn-Cu-V mixed-oxide regenerable sorbents for hot gas desulfurization
Karayilan, D; Doğu, Timur; Yasyerli, S; Dogu, G (American Chemical Society (ACS), 2005-07-06)
Porous Mn-Cu and Mn-Cu-V mixed-oxide sorbents prepared by the complexation technique were tested for high-temperature removal of H2S in the presence of hydrogen gas. Sorption experiments carried out at 627 T in a fixed-bed reactor showed higher reactivity and also higher sulfur retention capacity (over 0.15 g of S/g of sorbent) for the Mn-Cu mixed oxide than for the Mn-Cu-V mixed oxide. Successive sulfidation-regeneration cycles demonstrated that the MnCu mixed oxide was also more stable than Mn-Cu-V, maint...
MCMURRAY, L; HOLMES, AJ; KUPERMAN, A; OZIN, GA; Özkar, Saim (American Chemical Society (ACS), 1991-11-01)
Proton-loaded zeolites, prepared from fully dehydrated zeolites and gaseous, anhydrous Bronsted acids, represent an important step in the synthesis of intrazeolite semiconductor quantum supralattices. Adsorption-induced Na-23 MAS NMR chemical shifts, far-IR Na+ and Tl+ translatory mode frequency shifts, and Tl+ luminescence quenching effects were chosen as probes of cation-anion interaction in these materials. Samples of zeolite Y with various loadings of Tl+ were prepared via aqueous ion-exchange techniq...
Physical Characterization of Inclusion Complexes of Triphenyl Phosphate and Cyclodextrins in Solution.
Zhang, N; Zane, CP; Chen, Y; Yıldırım, Erol; Hinks, D; Tonelli, AE; Vinueza, NR; Pasquinelli, MA (American Chemical Society (ACS), 2020-01-16)
The goal of this work is to provide physical insights into the formation and stability of inclusion complexes (ICs) in aqueous solution between cyclodextrins (CDs) and a common flame retardant, triphenyl phosphate (TPP). Quantum chemistry calculations reveal the possible energetically favorable geometries of TPP in their 1:1 IC form with α-, β-, and γ-CDs as well as their associated complexation, conformational, and interaction energies. High-resolution mass spectrometry (MS) and tandem MS were used with el...
Citation Formats
G. Onur, F. Yılmaz, and B. İçgen, “Diesel Oil Degradation Potential of a Bacterium Inhabiting Petroleum Hydrocarbon Contaminated Surface Waters and Characterization of Its Emulsification Ability,” JOURNAL OF SURFACTANTS AND DETERGENTS, pp. 707–717, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/37306.