Synthesis of calcium carbonate microspheres via inert gas bubbling for orthopedic applications

Oral, Cagatay M.
Caliskan, Arda
Goctu, Yagmur
Ercan, Batur
Calcium carbonate (CaCO3) microspheres consisting of vaterite polymorph have been widely used in biomedical applications. Specifically, vaterite microspheres having hollow cores showed significant potential in drug delivery, however the spontaneous transformation of vaterite to other polymorphs in aqueous environments reduced its controlled in vivo release capability. In this work, calcite and aragonite microspheres having hollow/porous inner cores were synthesized -for the first time-using sodium dodecyl sulfate (SDS) stabilized nitrogen (N-2) bubbles as CaCO3 template in ethylene glycol (EG) solution and water as the precipitation medium. Results demonstrated that porous aragonite microspheres could be synthesized via N-2 gas incorporation, yet for the synthesis of hollow calcite microspheres, N-2 bubbles had be stabilized with SDS to be utilized as CaCO3 templates. The synthesized aragonite and calcite microspheres were found to be stable up to 5 days in Dulbecco's Modified Eagle's Medium (DMEM), and thus would not allow polymorphic transformation in aqueous environments, while promoting proliferation of human bone cells (hFOB) up to 5 days of culture. These findings -for the first time-identified a viable synthesis route for hollow/porous calcite and aragonite microspheres and indicated their promising use in orthopedic applications.


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Synthesis of calcium carbonate particles for biomedical applications
Oral, Çağatay Mert; Ercan, Batur; Department of Metallurgical and Materials Engineering (2020)
Calcium carbonate (CaCO3) particles have been widely used in biomedical applications owing to their biocompatibility and biodegradability. In order to effectively utilize CaCO3 particles in biomedical applications, their physical and chemical properties should be systematically controlled. However, this is a challenging task due to the presence of three different anhydrous CaCO3 polymorphs having complex crystallization behavior. In this thesis, CaCO3 particles were synthesized at distinct environments to c...
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Citation Formats
C. M. Oral, A. Caliskan, Y. Goctu, D. KAPUSUZ, and B. Ercan, “Synthesis of calcium carbonate microspheres via inert gas bubbling for orthopedic applications,” CERAMICS INTERNATIONAL, pp. 3513–3522, 2020, Accessed: 00, 2020. [Online]. Available: