Serum Glycan Signatures of Gastric Cancer

Date

2014-02-01

Author

Özcan Kabasakal, Süreyya
Ruhaak, L. Renee
Torres, Javier
Cooke, Cara L.
An, Hyun Joo
Hua, Serenus
Williams, Cynthia C.
Dimapasoc, Lauren M.
Kim, Jae Han
Camorlinga-Ponce, Margarita
Rocke, David
Lebrilla, Carlito B.
Solnick, Jay V.

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

318
views

0
downloads

Glycomics, a comprehensive study of glycans expressed in biologic systems, is emerging as a simple yet highly sensitive diagnostic tool for disease onset and progression. This study aimed to use glycomics to investigate glycan markers that would differentiate patients with gastric cancer from those with nonatrophic gastritis. Patients with duodenal ulcer were also included because they are thought to represent a biologically different response to infection with Helicobacter pylori, a bacterial infection that can cause either gastric cancer or duodenal ulcer. We collected 72 serum samples from patients in Mexico City that presented with nonatrophic gastritis, duodenal ulcer, or gastric cancer. N-glycans were released from serum samples using the generic method with PNGase F and were analyzed by matrix-assisted laser desorption/ionization Fourier transform-ion cyclotron resonance mass spectrometry. The corresponding glycan compositions were calculated based on accurate mass. ANOVA-based statistical analysis was performed to identify potential markers for each subgroup. Nineteen glycans were significantly different among the diagnostic groups. Generally, decreased levels of high-mannose-type glycans, glycans with one complex type antenna, bigalactosylated biantennary glycans, and increased levels of nongalactosylated biantennary glycans were observed in gastric cancer cases. Altered levels of serum glycans were also observed in duodenal ulcer, but differences were generally in the same direction as gastric cancer. Serum glycan profiles may provide biomarkers to differentiate gastric cancer cases from controls with nonatrophic gastritis. Further studies will be needed to validate these findings as biomarkers and identify the role of protein glycosylation in gastric cancer pathology. (C)2013 AACR.

Subject Keywords

Cancer Research, Oncology

URI

https://hdl.handle.net/11511/37957

Journal

CANCER PREVENTION RESEARCH

DOI

https://doi.org/10.1158/1940-6207.capr-13-0235

Collections

Department of Chemistry, Article

Suggestions

OpenMETU
Core

Significance of genetic Polymorphisms at multiple loci of CYP2E1 in the risk of development of childhood acute lymphoblastic leukemia Ulusoy, Gulen; Adalı, Orhan; Tumer, Tugba Boyunegmez; Sahin, Gurses; Gozdasoglu, Sevgi; Arinc, Emel (S. Karger AG, 2007-01-01) Background/Aims: The molecular etiology of childhood acute lymphoblastic leukemia (ALL) is likely to involve interactions between environmental factors and genetic make up. Understanding these interactions between various predisposing genes for the risk of developing childhood leukemia is of considerable importance. CYP2E1 is a susceptible gene in this respect, especially for its capacity to bioactivate many procarcinogens including benzene and N-nitrosodimethylamine. The CYP2E1 gene possesses several polym...
Extraction of auditory evoked potentials from ongoing eeg Aydın, Serap; Gençer, Nevzat Güneri; Department of Electrical and Electronics Engineering (2005) In estimating auditory Evoked Potentials (EPs) from ongoing EEG the number of sweeps should be reduced to decrease the experimental time and to increase the reliability of diagnosis. The rst goal of this study is to demon- strate the use of basic estimation techniques in extracting auditory EPs (AEPs) from small number of sweeps relative to ensemble averaging (EA). For this purpose, three groups of basic estimation techniques are compared to the traditional EA with respect to the signal-to-noise ratio(SNR) ...
Monoclonal antibodies for targeted therapy in colorectal cancer. Banerjee, Sreeparna (Informa UK Limited, 2010-04-15) Traditional therapeutic regimens of solid tumors such as chemotherapy and radiotherapy often do not distinguish between malignant and normal tissues, resulting in considerable side-effects. Monoclonal antibodies (mAbs), targeted against antigens dysregulated in cancers, have therefore generated great interest in both clinical and research settings. The antibodies are either chimeric or human(ized) and can bind to and inhibit target proteins overexpressed in both solid tumors and hematological malignancies. ...
Investigation of a mechanistic link between 15-Lipoxygenase-1 (15-LOX-1) and Metastasis Associated Protein 1 (MTA1) in human colorectal carcinoma cell lines ÇAĞATAY, SİBEL; Banerjee, Sreeparna (2013-09-01) Background: Metastasis Associated Protein 1 (MTA1) is a member of the nuclear remodeling and histone deacetylase (NuRD) complex that is known to repress the expression of several tumor suppressor genes. 15-lipoxygenase-1 (15-LOX-1), a member of the inflammatory eicosanoid pathway, has been shown by us and others to have an anti-tumorigenic role in colon cancer. ALOX15 was recently shown to be repressed by the NuRD complex. Previously we have reported that ectopic expression of 15-LOX-1 in colon cancer cells...
APA isoform diversity in triple negative breast cancers AKMAN TUNCER, HESNA BEGÜM; ÖYKEN, MERVE; AĞUŞ, HIZLAN HINCAL; Erdem, Murat; Çiçek, Esra; Can, Tolga; Erson Bensan, Ayşe Elif (2017-04-05) Alternative polyadenylation (APA) plays a role in gene expression regulation generally by shortening of 3'UTRs and relieving microRNA-mediated repression. Therefore, APA is gaining increased attention as a potential mechanism to activate oncogenes. Owing to high proliferative indices of triple negative breast cancers (TNBCs), we hypothesized APA to cause 3'UTR length changes in this aggressive subgroup of breast cancers. Our probe-based meta-analysis approach identified 3'UTR length alterations where the si...

Citation Formats

S. Özcan Kabasakal et al., “Serum Glycan Signatures of Gastric Cancer,” CANCER PREVENTION RESEARCH, pp. 226–235, 2014, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/37957.