In vivo examination of the effects of hydroxycinnamic acid on xenobiotic metabolizing and antioxidant enzymes

Download

10.2298-ABS160208083S.pdf

Date

2017-01-01

Author

SEMİZ, ASLI
Celik-Turgut, Gurbet
KARAKURT, SERDAR
AKÇA, HAKAN
ARSLAN, ŞEVKİ
Adalı, Orhan
ŞEN, ALAATTİN

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

183
views

98
downloads

In the last decade, hydroxycinnamic acids (HCA) have gained increasing attention from researchers due to their antioxidant potential. The aim of this study was to examine in detail the impact of dietary HCA on particular types of P450 and also selected phase II and antioxidant enzymes in Wistar rat. HCA (10 mu M/kg/day, i.p.) was administered for ten continuous days. Examination of the activities and mRNA and protein levels revealed that CYP2B, 2C6 and 3A enzyme activities were not altered significantly, with Western blot and qRT-PCR results corroborating this result. While treatment with HCA led to a significant reduction in CYP1A1/CYP1A2-associated enzyme activities, CYP1A1 protein, and mRNA levels were found to be unchanged. Aromatase (CYP19) activity, as well as protein and mRNA levels, were significantly reduced with HCA treatment. On the other hand, the NAD(P) H: quinone oxidoreductase 1 (NQO1), catalase (CAT), glutathione peroxidase (GPx) and glutathione S-transferases (GSTs) activities were increased significantly. Also, HCA treatment significantly increased the GST-mu and GST-theta mRNA levels.

Subject Keywords

General Biochemistry, Genetics and Molecular Biology, General Agricultural and Biological Sciences

URI

https://hdl.handle.net/11511/38334

Journal

ARCHIVES OF BIOLOGICAL SCIENCES

DOI

https://doi.org/10.2298/abs160208083s

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

A Comparative Study for the Evaluation of Two Doses of Ellagic Acid on Hepatic Drug Metabolizing and Antioxidant Enzymes in the Rat Celik, Gurbet; SEMİZ, ASLI; Karakurt, Serdar; ARSLAN, ŞEVKİ; Adalı, Orhan; ŞEN, ALAATTİN (Hindawi Limited, 2013-01-01) The present study was designed to evaluate different doses of ellagic acid (EA) in vivo in rats for its potential to modulate hepatic phases I, II, and antioxidant enzymes. EA (10 or 30 mg/kg/day, intragastrically) was administered for 14 consecutive days, and activity, protein, and mRNA levels were determined. Although the cytochrome P450 (CYP) 2B and CYP2E enzyme activities were decreased significantly, the activities of all other enzymes were unchanged with the 10 mg/kg/day EA. In addition, western-blot ...
FREE-RADICAL MEDIATED INTERACTION OF ASCORBIC-ACID AND ASCORBATE/CU(II) WITH VIRAL AND PLASMID DNAS ERDEM, G; ONER, C; Önal, Ahmet Muhtar; KISAKUREK, D; OGUS, A (Springer Science and Business Media LLC, 1994-03-01) Previous studies indicate that ascorbic acid, when combined with copper or iron cleaves several viral DNAs. In this study, we generated the ascorbate radical anion electrochemically in a simple chemical environment without the participation of a metal ion. This solution possesses viral DNA scission activity. The absence of catalytic metal ions [Fe (III) and Cu(II)] in the incubation medium was evidenced by metal chelating agents such as desferrioxamine and EDTA. The radical quenching at high EDTA concentrat...
Characterising Complex Enzyme Reaction Data Dönertaş, Handan Melike; Cuesta, Sergio Martínez; Rahman, Syed Asad; Thornton, Janet M. (Public Library of Science (PLoS), 2016-2-3) The relationship between enzyme-catalysed reactions and the Enzyme Commission (EC) number, the widely accepted classification scheme used to characterise enzyme activity, is complex and with the rapid increase in our knowledge of the reactions catalysed by enzymes needs revisiting. We present a manual and computational analysis to investigate this complexity and found that almost one-third of all known EC numbers are linked to more than one reaction in the secondary reaction databases (e.g., KEGG). Although...
An alternative supporting electrolyte for enzyme immobilization in conducting polymers Kiralp, Senem; Balik, Balam; KARATAŞ, SEVİM; Toppare, Levent Kamil; Gungorb, Atilla (Elsevier BV, 2008-03-01) In this study an alternative supporting electrolyte was used in enzyme immobilization. Invertase was studied to observe the effect of the supporting electrolyte. Sulfonated poly(arylene ether sulfone) was used as the supporting electrolyte during the electrolysis of pyrrole. The results show that the polymeric supporting electrolyte can be used instead of sodium dodecyl sulfate.
Collagen/PEO/gold nanofibrous matrices for skin tissue engineering Akturk, Omer; Keskin, Dilek (The Scientific and Technological Research Council of Turkey, 2016-01-01) As a novel approach in skin tissue engineering, gold nanoparticles (AuNPs) were synthesized and incorporated at different concentrations into collagen/PEO nanofibrous matrices in this study. The group containing 14.27 ppm AuNPs (CM-Au) had the best nanofibrous morphology. CM-Au was cross-linked with glutaraldehyde vapor (CM-AuX). All groups were disrupted in collagenase in 2 h, but cross-linked groups and Matriderm (R) resisted hydrolytic degradation for 7 and 14 days, respectively. Due to its small pores a...

Citation Formats

A. SEMİZ et al., “In vivo examination of the effects of hydroxycinnamic acid on xenobiotic metabolizing and antioxidant enzymes,” ARCHIVES OF BIOLOGICAL SCIENCES, pp. 103–110, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/38334.