In vivo examination of the effects of hydroxycinnamic acid on xenobiotic metabolizing and antioxidant enzymes

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10.2298-ABS160208083S.pdf

Date

2017-01-01

Author

SEMİZ, ASLI
Celik-Turgut, Gurbet
KARAKURT, SERDAR
AKÇA, HAKAN
ARSLAN, ŞEVKİ
Adalı, Orhan
ŞEN, ALAATTİN

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In the last decade, hydroxycinnamic acids (HCA) have gained increasing attention from researchers due to their antioxidant potential. The aim of this study was to examine in detail the impact of dietary HCA on particular types of P450 and also selected phase II and antioxidant enzymes in Wistar rat. HCA (10 mu M/kg/day, i.p.) was administered for ten continuous days. Examination of the activities and mRNA and protein levels revealed that CYP2B, 2C6 and 3A enzyme activities were not altered significantly, with Western blot and qRT-PCR results corroborating this result. While treatment with HCA led to a significant reduction in CYP1A1/CYP1A2-associated enzyme activities, CYP1A1 protein, and mRNA levels were found to be unchanged. Aromatase (CYP19) activity, as well as protein and mRNA levels, were significantly reduced with HCA treatment. On the other hand, the NAD(P) H: quinone oxidoreductase 1 (NQO1), catalase (CAT), glutathione peroxidase (GPx) and glutathione S-transferases (GSTs) activities were increased significantly. Also, HCA treatment significantly increased the GST-mu and GST-theta mRNA levels.

Subject Keywords

General Biochemistry, Genetics and Molecular Biology, General Agricultural and Biological Sciences

URI

https://hdl.handle.net/11511/38334

Journal

ARCHIVES OF BIOLOGICAL SCIENCES

DOI

https://doi.org/10.2298/abs160208083s

Collections

Department of Biology, Article

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Citation Formats

A. SEMİZ et al., “In vivo examination of the effects of hydroxycinnamic acid on xenobiotic metabolizing and antioxidant enzymes,” ARCHIVES OF BIOLOGICAL SCIENCES, pp. 103–110, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/38334.