Collagen/PEO/gold nanofibrous matrices for skin tissue engineering

Date

2016-01-01

Author

Akturk, Omer
Keskin, Dilek

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As a novel approach in skin tissue engineering, gold nanoparticles (AuNPs) were synthesized and incorporated at different concentrations into collagen/PEO nanofibrous matrices in this study. The group containing 14.27 ppm AuNPs (CM-Au) had the best nanofibrous morphology. CM-Au was cross-linked with glutaraldehyde vapor (CM-AuX). All groups were disrupted in collagenase in 2 h, but cross-linked groups and Matriderm (R) resisted hydrolytic degradation for 7 and 14 days, respectively. Due to its small pores and dense structure, lower water swelling results (7.26 +/- 2.62 g/g) were obtained for CM-AuX than Matriderm (17.51 +/- 1.97 g/g). CM-Au and Matriderm had statistically similar tensile strength and elastic modulus, but elongation at break of CM-Au (over 100%) was significantly better than that of Matriderm. After cross-linking, tensile strength and elastic modulus of collagen matrix was further improved. AuNPs (37 and 42 nm) seemed to be nontoxic on 3T3 fibroblasts and keratinocytes for different time periods. CM-AuX scaffold extracts were also nontoxic for 3T3 fibroblasts and keratinocytes. The L929 cell attachment and proliferation on CM-AuX were comparable with Matriderm, indicating good in vitro biocompatibility. As a whole, collagen matrices incorporated with AuNPs are potential biomaterial candidates for skin tissue engineering.

Subject Keywords

Genetics, Cell Biology, Physiology, Molecular Biology, General Agricultural and Biological Sciences, Microbiology

URI

https://hdl.handle.net/11511/43039

Journal

TURKISH JOURNAL OF BIOLOGY

DOI

https://doi.org/10.3906/biy-1502-49

Collections

Department of Engineering Sciences, Article

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Citation Formats

O. Akturk and D. Keskin, “Collagen/PEO/gold nanofibrous matrices for skin tissue engineering,” TURKISH JOURNAL OF BIOLOGY, pp. 380–398, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/43039.