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FREE-RADICAL MEDIATED INTERACTION OF ASCORBIC-ACID AND ASCORBATE/CU(II) WITH VIRAL AND PLASMID DNAS
Date
1994-03-01
Author
ERDEM, G
ONER, C
Önal, Ahmet Muhtar
KISAKUREK, D
OGUS, A
Metadata
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This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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Previous studies indicate that ascorbic acid, when combined with copper or iron cleaves several viral DNAs. In this study, we generated the ascorbate radical anion electrochemically in a simple chemical environment without the participation of a metal ion. This solution possesses viral DNA scission activity. The absence of catalytic metal ions [Fe (III) and Cu(II)] in the incubation medium was evidenced by metal chelating agents such as desferrioxamine and EDTA. The radical quenching at high EDTA concentration was attributed to ionic strength of EDTA rather than metal chelation. The effects of antioxidants, radical scavangers, catalase, superoxide dismutase and some proteins on DNA cleavage have been tested. Cleavage may not arise directly from ascorbate free radical but the reaction of the radical form of ascorbate with oxygen may produce the actual reactive species. Aerobic oxidation of ascorbate itself strictly requires transition metal catalysts, however electrochemically produced ascorbyl radical avoided the kinetic barrier that prevented direct oxidation of ascorbic acid with oxygen and eliminated the need for the transition metal ion catalysts.
Subject Keywords
General Biochemistry, Genetics and Molecular Biology
,
General Agricultural and Biological Sciences
,
General Medicine
URI
https://hdl.handle.net/11511/48944
Journal
JOURNAL OF BIOSCIENCES
DOI
https://doi.org/10.1007/bf02703463
Collections
Department of Chemistry, Article