Expression profiling in response to ascochyta rabiei inoculations in chickpea

Download

index.pdf

Date

2008

Author

Avcıoğlu Dündar, Banu

Metadata

Show full item record

Item Usage Stats

312
views

237
downloads

In this study, it was aimed to identify chickpea (Cicer arietinum) genes or gene fragments expressed upon Ascochyta rabiei infection using a tolerant chickpea cultivar ILC195 and fungal isolates with varying level of pathogenicity. PCR amplification of resistance gene analogs (RGA) and disease related genes, and mRNA differential display reverse transcription (DDRT) were used to get these expressed gene fragments in chickpea. The constitutively or differentially expressed PCR product fragments were cloned and sequenced. Out of nearly 300 clones, 160 sequences (expressed sequence tags, ESTs) could be analyzed and these sequences were disclosed in this study. About 100 of these ESTs were classified according to predicted “molecular function”, “biological process” and “cellular component”. The most common ppredicted functions of the products coded by these ESTs were “Protein Fate”, “Metabolism”, “Cell Rescue, Defense and Virulence”, “Transcription”, “Transport”, “Energy”, and “Cell Fate”. Six ESTs were subjected to Real-Time quantitative RT-PCR analysis to compare the response of ILC195 infected by one A.rabiei isolate with another resistant chickpea genotype (FLIP84-92C)/A.rabiei pathotype system. Some of these genes were differentially expressed among different chickpea/A.rabiei isolate combinations. Highly upregulated ESTs in all these combinations were a formate dehydrogenase (metabolism and detoxification), a serine carboxypeptidase (protein fate and communication) and a hypothetical protein probably similar to acyl-CoA synthetases. A genetic mapping study was carried out with EST specific primers and two EST markers were assigned in the current chickpea genetic map. However, no genetic linkage of them was detected with known chickpea quantitative trait loci for A.rabiei resistance.

Subject Keywords

General biology., Plant physiology.

URI

http://etd.lib.metu.edu.tr/upload/2/12610091/index.pdf
https://hdl.handle.net/11511/18010

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Effect of salt stress on antioxidant defense systems of sensitive and resistance cultivars of lentil(Lens culinaris M.) Cicerali, Işın Nur; Yücel, Ayşe Meral; Department of Biotechnology (2004) In this study, two lentil cultivars (Lens culinaris, Medik.) (ILL5582-salt tolerant and ILL590) were characterized and compared due to their NaCl susceptibility and antioxidant mechanism was examined under laboratory conditions. Physiological parameters such as wet-dry weight, root-shoot lengths, cell membrane stability, lipid peroxidation in terms of malondialdehyde (MDA), H2O2, proline contents were determined. The activities of antioxidant enzymes such as superoxide dismutase (SOD: EC 1.15.1.1), catalase...
Evaluation of photosynthetic performance of wheat cultivars exposed to boron toxicity by the JIP fluorescence test Oz, M. T.; TURAN, ÖZLEM; Kayihan, C.; EYİDOĞAN, FÜSUN; EKMEKÇİ, YASEMİN; YÜCEL, MUSTAFA; Öktem, Hüseyin Avni (Institute of Experimental Botany, 2014-12-01) The changes in growth and photosynthetic performance of two wheat (Triticum aestivum L.) cultivars (Bolal-2973 and Atay-85) differing in their sensitivity to boron (B) toxicity were investigated under toxic B conditions. Eight-day old seedlings were exposed to highly toxic B concentrations (5, 7.5, and 10 mM H3BO3) for 5 and 9 days. Fast chlorophyll a fluorescence kinetics was determined and analysed using JIP test. Growth parameters, tissue B contents, and membrane damage were measured at two stress durati...
Effect of drought and salt stresses on antioxidant defense system and physiology of lentil (lens culinaris m.) seedlings Ercan, Oya; Öktem, Hüseyin Avni; Department of Biology (2008) In this study, 14 days old lentil seedlings (Lens culinaris Medik cv. Sultan), which were subjected to 7 days of drought (20% PEG 6000), and salt (150 mM NaCl ) stress , were examined in a comparative manner for the effects of drought and salt stress treatments. In shoot and root tissues physiological parameters such as wet-dry weight, relative water content, root-shoot lengths, membrane electrolyte leakage, and lipid peroxidation in terms of malondialdehyde (MDA) were determined. H2O2 content, proline accu...
CpG oligodeoxynucleotides protect normal and SIV-infected macaques from Leishmania infection. Verthelyi, D; Gürsel, Mayda; Kenney, RT; Lifson, JD; Liu, S; Mican, J; Klinman, DM (The American Association of Immunologists, 2003-05-01) Oligodeoxynucleotides containing CpG motifs (CpG ODNs) mimic microbial DNA and activate effectors of the innate immune response, which limits the spread of pathogens and promotes an adaptive immune response. CpG ODNs have been shown to protect mice from infection with intracellular pathogens. Unfortunately, CpG motifs that optimally stimulate humans are only weakly active in mice, mandating the use of nonhuman primates to monitor the activity and safety of "human" CpG ODNs in vivo. This study demonstrates t...
ANALYSIS OF PLASMIDS MEDIATING ANT (2'') AND AAC(3)-II BASED GENTAMICIN RESISTANCE IN CLINICAL ISOLATES OF ENTEROBACTERIACEAE Kocabıyık, Semra (Elsevier BV, 1989-07-01) The genetics of ANT(2″) and AAC(3)-II mediated gentamicin resistance in Gram-negative bacteria obtained from Hacettepe University Hospital in Turkey, was studied. The plasmid DNA analyzed by agarose gel electrophoresis revealed a heterogeneous plasmid population. Conjugal transfer appeared to be an efficient mechanism for the dissemination of resistance to gentamicin/tobramycin/kanamycin/streptomycin/chloramphenicol/cephalothin, which was the common resistance pattern in the population. Plasmids of 46, 60, ...

Citation Formats

B. Avcıoğlu Dündar, “Expression profiling in response to ascochyta rabiei inoculations in chickpea,” Ph.D. - Doctoral Program, Middle East Technical University, 2008.