Effect of vitamin C and lipoic acid on streptozotocin-induced diabetes gene expression: mRNA and protein expressions of Cu-ZnSOD and catalase

Sadi, Goekhan
Yilmaz, Oekkes
Güray, Nülüfer Tülün
The involvement of oxidative stress in the pathogenesis of diabetes mellitus has been confirmed by numerous studies. In this study, the expression of two antioxidant enzymes, superoxide dismutase (SOD), and catalase which are involved in the detoxification of reactive oxygen species was studied in the streptozotocin-induced diabetic rat liver tissues. The enzyme assays showed a significant decrease in both enzymes activities compared to control animals. The RT-PCR and Western-blot analysis results demonstrated that this decrease in activity is regulated at the level of gene expression, as both catalase and Cu-Zn SOD mRNA and protein expressions were also suppressed. Supplementing the animals with vitamin C, a powerful antioxidant increased both SOD and catalase activities with no change in both mRNA and protein expressions suggesting a role of post-translational modification. However, even though mRNA expressions of both catalase and Cu-Zn SOD were not changed, the protein levels increased in parallel to activities in the case of another antioxidant, alpha-lipoic acid. An increase in the rate of translation, without changing the rate of transcription indicates a translational effect of lipoic acid in changing the activities of antioxidant enzymes to prevent the oxidative damage in diabetes.


Gene expressions of Mn-SOD and GPx-1 in streptozotocin-induced diabetes: effect of antioxidants
Sadi, Goekhan; Güray, Nülüfer Tülün (Springer Science and Business Media LLC, 2009-07-01)
Increased oxidative stress and impaired antioxidant defense mechanisms are believed to be the important factors contributing to the pathogenesis and progression of diabetes mellitus. In this study, we have reported the effects of the streptozotocin-induced diabetes on the gene expression and the activities of two antioxidant enzymes, manganese superoxide dismutase (MnSOD) and glutathione peroxidase (GPx). We also studied the effects of two antioxidants, vitamin C and DL-alpha-lipoic acid (LA), on the system...
Effect of quercetin on mRNA and protein expressions of vitamin D metabolizing CYP27B1 and CYP24A1 in human embryonic kidney cell line (HEK-293)
Durukan, Özlem; Akkulak, Merve; Özhan, Güneş; Evin, Emre; Adalı, Orhan (Wiley, 2018-07-01)
Vitamin D is essential compound for life having role not only in the regulation of calcium metabolism but also in the regulation of cell proliferation, immune responses, cardiovascular homeostasis and nervous system. These wide range of actions occur with the enzymatic conversion of vitamin D to 1a,25-dihydroxyvitamin D by cytochrome P450 enzymes (CYPs). While CYP27B1 provides the synthesis of hormonally active form of Vitamin D, 1,25(OH)2D, CYP24A1 involves in catabolism of vitamin D. Quercetin, as one of ...
Effect of drought and salt stresses on the gene expression levels of antioxidant enzymes in lentil (lens culinaris m.) seedlings
Aksoy, Emre; Yücel, Ayşe Meral; Department of Biology (2008)
This study was carried out for understanding of antioxidant mechanisms of lentil under abiotic stress conditions. For this aim, 14 days old lentil seedlings (Lens culinaris Medik cv. Sultan-1) were subjected to drought (20% PEG 6000), and salt (150 mM NaCl ) stress for 6, 12 and 24 hours, for 3, 5 and 7 days. PCR conditions for Mn SOD, Cu/Zn SOD, chloroplastic/mitochondrial GR, CAT and chloroplast /stromal APX antioxidant enzymes were optimized. Then, total RNA was isolated from stressed and non-stressed pl...
Effects of benzene on liver, kidney and lung cyp1a, cyp2b4, cyp2e1 and cyp3a6 mrna, protein level, and drug metabolizing enzyme activities and toxicity in diabetic rabbits
Arslan, Şevki; Arınç, Emel; Department of Biochemistry (2008)
The effects of diabetes on cytochrome P450 dependent drug metabolizing enzymes have not to be clarified yet. The most widely used animals in these studies have been rats, and information regarding the effects of diabetes on cytochrome P450 dependent procarcinogen/carcinogen metabolism in rabbits is limited. In the present study, we investigated, for the first time, the influence of benzene on liver, kidney and lung microsomal cytochrome P450 dependent drug metabolizing enzyme activities, protein and mRNA le...
Apolipoprotein A5 polymorphisms in Turkish population: association with serum lipid profile and risk of ischemic stroke
CAN DEMİRDÖĞEN, BİRSEN; Sahin, Esra; Ozcelik, Aysun Turkanoglu; Bek, Semai; Demirkaya, Seref; Adalı, Orhan (Springer Science and Business Media LLC, 2012-12-01)
Atherosclerosis, a major cause of ischemic stroke, may be associated with variability of triglyceride (TG) levels. Apolipoprotein A5 (APOA5) genetic polymorphisms are associated with altered TG levels. The objective of this study was to investigate the coding region polymorphisms S19W (rs3135506) and G185C (rs2075291) and the promoter region polymorphism -1131T > C (rs662799) of the APOA5 gene as risk factors for ischemic stroke in Turkish population. Study group consisted of 272 ischemic stroke patients an...
Citation Formats
G. Sadi, O. Yilmaz, and N. T. Güray, “Effect of vitamin C and lipoic acid on streptozotocin-induced diabetes gene expression: mRNA and protein expressions of Cu-ZnSOD and catalase,” MOLECULAR AND CELLULAR BIOCHEMISTRY, pp. 109–116, 2008, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/40066.