USP32 regulates late endosomal transport and recycling through deubiquitylation of Rab7

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10.1038.s41467-019-09437-x.pdf

Date

2019-03-29

Author

Sapmaz, Aysegul
Berlin, Ilana
Bos, Erik
Wijdeven, Ruud H.
Janssen, Hans
Konietzny, Rebecca
Akkermans, Jimmy J.
Erson Bensan, Ayşe Elif
Koning, Roman
Kessler, Benedikt M.
Neefjes, Jacques
Ovaa, Huib

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The endosomal system is a highly dynamic multifunctional organelle, whose complexity is regulated in part by reversible ubiquitylation. Despite the wide-ranging influence of ubiquitin in endosomal processes, relatively few enzymes utilizing ubiquitin have been described to control endosome integrity and function. Here we reveal the deubiquitylating enzyme (DUB) ubiquitin-specific protease 32 (USP32) as a powerful player in this context. Loss of USP32 inhibits late endosome (LE) transport and recycling of LE cargos, resulting in dispersion and swelling of the late compartment. Using SILAC-based ubiquitome profiling we identify the small GTPase Rab7-the logistical centerpiece of LE biology-as a substrate of USP32. Mechanistic studies reveal that LE transport effector RILP prefers ubiquitylation-deficient Rab7, while retromer-mediated LE recycling benefits from an intact cycle of Rab7 ubiquity-lation. Collectively, our observations suggest that reversible ubiquitylation helps switch Rab7 between its various functions, thereby maintaining global spatiotemporal order in the endosomal system.

Subject Keywords

General Biochemistry, Genetics and Molecular Biology, General Physics and Astronomy, General Chemistry

URI

https://hdl.handle.net/11511/40211

Journal

NATURE COMMUNICATIONS

DOI

https://doi.org/10.1038/s41467-019-09437-x

Collections

Department of Biology, Article

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Citation Formats

A. Sapmaz et al., “USP32 regulates late endosomal transport and recycling through deubiquitylation of Rab7,” NATURE COMMUNICATIONS, pp. 0–0, 2019, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/40211.