Development of a solid-phase microextraction LC-MS/MS method for determination of oxidative stress biomarkers in biofluids

Date

2020-04-03

Author

Kahremanoglu, Kubra
Temel, Ezgi Rana
Korkut, Tamara Ecem
Nalbant, Atakan Arda
Baştuğ Azer, Bersu
Durucan, Caner
Volkan, Mürvet
Boyacı, Ezel

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

336
views

0
downloads

Recently the connection between oxidative stress and various diseases, including cancer and Alzheimer's, attracts notice as a pathway suitable for diagnostic purposes. 8-Oxo-deoxyguanosine and 8-oxo-deoxyadenosine produced from the interaction of reactive oxygen species with DNA become prominent as biomarkers. Several methods have been developed for their determination in biofluids, including solid-phase extraction and enzyme-linked immunosorbent assays. However, still, there is a need for reliable and fast analytical methods. In this context, solid-phase microextraction offers many advantages such as flexibility in geometry and applicable sample volume, as well as high adaptability to high-throughput sampling. In this study, a solid-phase microextraction method was developed for the determination of 8-oxo-deoxyguanosine and 8-oxo-deoxyadenosine in biofluids. The extractive phase of solid-phase microextraction consisted of hydrophilic-lipophilic balanced polymeric particles. In order to develop a solid-phase microextraction method suitable for the determination of the analytes in saliva and urine, several parameters, including desorption solvent, desorption time, sample pH, and ionic strength, were scrutinized. Analytical figures of merit indicated that the developed method provides reasonable interday and intraday precisions (<15% in both biofluids) with acceptable accuracy. The method provides a limit of quantification for both biomarkers at 5.0 and 10.0 ng/mL levels in saliva and urine matrices, respectively.

Subject Keywords

Filtration and Separation, Analytical Chemistry

URI

https://hdl.handle.net/11511/40333

Journal

JOURNAL OF SEPARATION SCIENCE

DOI

https://doi.org/10.1002/jssc.202000211

Collections

Department of Metallurgical and Materials Engineering, Article

Suggestions

OpenMETU
Core

Comprehensive native glycan profiling with isomer separation and quantitation for the discovery of cancer biomarkers Hua, Serenus; An, Hyun Joo; Özcan Kabasakal, Süreyya; Ro, Grace S.; Soares, Stephanie; DeVere-White, Ralph; Lebrilla, Carlito B. (Royal Society of Chemistry (RSC), 2011-01-01) Glycosylation is highly sensitive to the biochemical environment and has been implicated in many diseases including cancer. Glycan compositional profiling of human serum with mass spectrometry has already identified potential biomarkers for several types of cancer and diseases; however, composition alone does not fully describe glycan stereo-and regioisomeric diversity. The vast structural heterogeneity of glycans presents a formidable analytical challenge. We have developed a method to identify and quantif...
Label-free detection of telomerase activity using guanine electrochemical oxidation signal Eskiocak, Ugur; Ozkan-Ariksoysal, Dilsat; Ozsoz, Mehmet; Öktem, Hüseyin Avni (American Chemical Society (ACS), 2007-11-15) Telomerase is an important biomarker for cancer cells and its activation in 85% of all cancer types confers a clinical diagnostic value. A label-free electrochemical assay based on guanine oxidation signal to measure telomerase activity is described. This developed technology combined with a disposable sensor, carbon graphite electrode (CGE), and differential pulse voltammetry (DPV) was performed by using PCR amplicons with/without telomeric repeats as the guanine oxidation signal observed at +1.0 V measure...
Evaluation and discrimination of simvastatin-induced structural alterations in proteins of different rat tissues by FTIR spectroscopy and neural network analysis Garip, Sebnem; Yapici, Engin; Ozek, Nihal Simsek; Severcan, Mete; Severcan, Feride (Royal Society of Chemistry (RSC), 2010-01-01) Statins are commonly used to control hypercholesterolemia and to prevent cardiovascular diseases. Among the statins, Simvastatin is one of the most frequently prescribed statins because of its efficacy in reducing LDL lipoprotein cholesterol levels, its tolerability, and its reduction of cardiovascular risk and mortality. Conflicting results have been reported with regard to benefits (pleiotropic effects) as well as risks (adverse effects) of simvastatin on different soft and hard tissues. In the current st...
In-Depth Method for the Characterization of Glycosylation in Manufactured Recombinant Monoclonal Antibody Drugs Song, Ting; Özcan Kabasakal, Süreyya; Becker, Alicia; Lebrilla, Carlito B. (American Chemical Society (ACS), 2014-06-17) The glycosylation in recombinant monoclonal antibody (rMab) drugs is a major concern in the biopharmaceutical industry as it impacts the drugs' many attributes. Characterization is important but complicated by the intricate structures, microheterogeneity, and the limitations of current tools for structural analysis. In this study, we developed a liquid chromatography-mass spectrometry (LC-MS) N-glycan library based on eight commercial rMab drugs. A library of over 70 structures was developed for the rapid c...
Numerical modeling and experimental investigation of cell manipulation using acoustophoresis Karaman, Alara; Özer, Mehmet Bülent; Department of Mechanical Engineering (2022-8-24) Several diseases, such as sickle cell disease and cancer, can affect the properties of cells. Manipulation or separation of the altered cell is necessary to diagnose such diseases. Acoustophoresis, the migration of particles by using acoustic standing waves, is a promising technique for bio-particle manipulation. This thesis presents numerical modelling and experimental investigation of using acoustophoresis for cell manipulation purposes. First, the trajectories of the cells in an acoustophoretic chip are ...

Citation Formats

K. Kahremanoglu et al., “Development of a solid-phase microextraction LC-MS/MS method for determination of oxidative stress biomarkers in biofluids,” JOURNAL OF SEPARATION SCIENCE, pp. 0–0, 2020, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/40333.