Development of a solid-phase microextraction LC-MS/MS method for determination of oxidative stress biomarkers in biofluids

Date

2020-04-03

Author

Kahremanoglu, Kubra
Temel, Ezgi Rana
Korkut, Tamara Ecem
Nalbant, Atakan Arda
Baştuğ Azer, Bersu
Durucan, Caner
Volkan, Mürvet
Boyacı, Ezel

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Recently the connection between oxidative stress and various diseases, including cancer and Alzheimer's, attracts notice as a pathway suitable for diagnostic purposes. 8-Oxo-deoxyguanosine and 8-oxo-deoxyadenosine produced from the interaction of reactive oxygen species with DNA become prominent as biomarkers. Several methods have been developed for their determination in biofluids, including solid-phase extraction and enzyme-linked immunosorbent assays. However, still, there is a need for reliable and fast analytical methods. In this context, solid-phase microextraction offers many advantages such as flexibility in geometry and applicable sample volume, as well as high adaptability to high-throughput sampling. In this study, a solid-phase microextraction method was developed for the determination of 8-oxo-deoxyguanosine and 8-oxo-deoxyadenosine in biofluids. The extractive phase of solid-phase microextraction consisted of hydrophilic-lipophilic balanced polymeric particles. In order to develop a solid-phase microextraction method suitable for the determination of the analytes in saliva and urine, several parameters, including desorption solvent, desorption time, sample pH, and ionic strength, were scrutinized. Analytical figures of merit indicated that the developed method provides reasonable interday and intraday precisions (<15% in both biofluids) with acceptable accuracy. The method provides a limit of quantification for both biomarkers at 5.0 and 10.0 ng/mL levels in saliva and urine matrices, respectively.

Subject Keywords

Filtration and Separation, Analytical Chemistry

URI

https://hdl.handle.net/11511/40333

Journal

JOURNAL OF SEPARATION SCIENCE

DOI

https://doi.org/10.1002/jssc.202000211

Collections

Department of Metallurgical and Materials Engineering, Article

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Citation Formats

K. Kahremanoglu et al., “Development of a solid-phase microextraction LC-MS/MS method for determination of oxidative stress biomarkers in biofluids,” JOURNAL OF SEPARATION SCIENCE, pp. 0–0, 2020, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/40333.