Comprehensive native glycan profiling with isomer separation and quantitation for the discovery of cancer biomarkers

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Date

2011-01-01

Author

Hua, Serenus
An, Hyun Joo
Özcan Kabasakal, Süreyya
Ro, Grace S.
Soares, Stephanie
DeVere-White, Ralph
Lebrilla, Carlito B.

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Glycosylation is highly sensitive to the biochemical environment and has been implicated in many diseases including cancer. Glycan compositional profiling of human serum with mass spectrometry has already identified potential biomarkers for several types of cancer and diseases; however, composition alone does not fully describe glycan stereo-and regioisomeric diversity. The vast structural heterogeneity of glycans presents a formidable analytical challenge. We have developed a method to identify and quantify isomeric native glycans using nanoflow liquid chromatography (nano-LC)/mass spectrometry. A microfluidic chip packed with graphitized carbon was used to chromatographically separate the glycans. To determine the utility of this method for structure-specific biomarker discovery, we analyzed serum samples from two groups of prostate cancer patients with different prognoses. More than 300 N-glycan species (including isomeric structures) were identified, corresponding to over 100 N-glycan compositions. Statistical tests established significant differences in glycan abundances between patient groups. This method provides comprehensive, selective, and quantitative glycan profiling.

Subject Keywords

Analytical Chemistry, Spectroscopy, Electrochemistry, Biochemistry, Environmental Chemistry

URI

https://hdl.handle.net/11511/46955

Journal

ANALYST

DOI

https://doi.org/10.1039/c1an15093f

Collections

Department of Chemistry, Article

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Citation Formats

S. Hua et al., “Comprehensive native glycan profiling with isomer separation and quantitation for the discovery of cancer biomarkers,” ANALYST, pp. 3663–3671, 2011, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/46955.