Numerical modeling and experimental investigation of cell manipulation using acoustophoresis

2022-8-24
Karaman, Alara
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Several diseases, such as sickle cell disease and cancer, can affect the properties of cells. Manipulation or separation of the altered cell is necessary to diagnose such diseases. Acoustophoresis, the migration of particles by using acoustic standing waves, is a promising technique for bio-particle manipulation. This thesis presents numerical modelling and experimental investigation of using acoustophoresis for cell manipulation purposes. First, the trajectories of the cells in an acoustophoretic chip are calculated using an acousto-mechanical numerical model that includes elastic modelling of cells. For experimental investigations, two different types of chip architecture are discussed. Using the first architecture, the capabilities of different chip materials for acoustic manipulation have been assessed by conducting thermal, vibration, focusing, and viability experiments. Then, a two-layered chip is introduced as a second architecture for bacteria and WBC separation purposes.
Acoustophoresis, Cell manipulation, Cancer cell separation
https://hdl.handle.net/11511/99371
Graduate School of Natural and Applied Sciences, Thesis

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Citation Formats
A. Karaman, “Numerical modeling and experimental investigation of cell manipulation using acoustophoresis,” M.S. - Master of Science, Middle East Technical University, 2022.
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