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Numerical modeling and experimental investigation of cell manipulation using acoustophoresis
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Numerical_Modeling_and_Experimental_Investigation_of_Cell_Manipulation_using_Acoustophoresis_Final.pdf
Date
2022-8-24
Author
Karaman, Alara
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Several diseases, such as sickle cell disease and cancer, can affect the properties of cells. Manipulation or separation of the altered cell is necessary to diagnose such diseases. Acoustophoresis, the migration of particles by using acoustic standing waves, is a promising technique for bio-particle manipulation. This thesis presents numerical modelling and experimental investigation of using acoustophoresis for cell manipulation purposes. First, the trajectories of the cells in an acoustophoretic chip are calculated using an acousto-mechanical numerical model that includes elastic modelling of cells. For experimental investigations, two different types of chip architecture are discussed. Using the first architecture, the capabilities of different chip materials for acoustic manipulation have been assessed by conducting thermal, vibration, focusing, and viability experiments. Then, a two-layered chip is introduced as a second architecture for bacteria and WBC separation purposes.
Subject Keywords
Acoustophoresis
,
Cell manipulation
,
Cancer cell separation
URI
https://hdl.handle.net/11511/99371
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Graduate School of Natural and Applied Sciences, Thesis
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A. Karaman, “Numerical modeling and experimental investigation of cell manipulation using acoustophoresis,” M.S. - Master of Science, Middle East Technical University, 2022.