Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
PREPARATION AND CHARACTERIZATION OF POLYMER COATED SMALL UNILAMELLAR VESICLES
Date
1991-09-02
Author
OZDEN, MY
Hasırcı, Vasıf Nejat
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
264
views
0
downloads
Cite This
Glucose oxidase was entrapped in small unilamellar vesicles composed of phosphatidylcholine, dicetyl phosphate and cholesterol. Prediction of the enzyme content of liposomes by calculations based on input concentrations of lipid and protein, dimensions of the lipids and the liposomes yielded one protein per vesicle. The entrapment efficiency was experimentally determined to be about 13%. On the other hand the entrapment efficiency for the small chromate ions was found to be significantly lower (0.1%). The liposomes were then coated with a polymer, poly(1,4-pyridinium diylethylene salt). It was possible to remove the lipoid material from underneath the polymer layer with various techniques. The effect of sonication and treatment with organic solvents (tested for this purpose) on enzyme activity were found to be very significant and Triton X-100 was chosen for this purpose. It was shown that the enzyme within the remaining net has 89% of its original activity.
Subject Keywords
Small unilamellar vesicle
,
Enzyme entrapment
,
Polymer coated vesicle
URI
https://hdl.handle.net/11511/30517
Journal
BIOCHIMICA ET BIOPHYSICA ACTA
DOI
https://doi.org/10.1016/0304-4165(91)90081-q
Collections
Graduate School of Natural and Applied Sciences, Article
Suggestions
OpenMETU
Core
Imaging of liposomes by scanning tunneling microscopy
Zareie, MH; Borucu, A; Ozden, MY; Hasırcı, Vasıf Nejat; Piskin, E (1996-01-01)
In this study, unilamellar and multilamellar liposomes consisting of dipalmitoylphosphatidylcholine, cholesterol and dicetylphosphate were prepared. Scanning tunneling microscopy images of these liposomes on highly oriented pyrolytic graphite surfaces were obtained. It was observed that scanning tunnelling microscopy can be succesfully used for high-resolution, three-dimensional structure analysis of liposomes under very mild conditions.
Optimization of mannanase production from recombinant aspergillus sojae and analysis of galactomannan hydrolysis
Öztürk, Bengü; Ögel, Zümrüt Begüm; Department of Food Engineering (2008)
Aspergillus fumigatus produces enzymes required for the hydrolysis of galactomannans like locust bean gum. Among these enzymes endo-beta-1,4 mannanase is also produced at high levels. However, the fungus is not safe for use in the food industry. Therefore, the gene encoding endo-beta-1,4-mannanase of A. fumigatus IMI 385708 was previously cloned in our laboratory into Aspergillus sojae ATCC11906 which is a safe microorganism for use in food applications. Altogether eight transformants were obtained. It was ...
Preparation and Characterization of Amino-Functionalized Zeolite/SiO2 Materials for Trypsin-Chymotrypsin Co-immobilization
Dogan, Demet; Sezer, Selda; Ulu, Ahmet; KÖYTEPE, SÜLEYMAN; ATEŞ, BURHAN (2021-08-01)
Inorganic supports have attracted increased attention in enzyme immobilization since they not only improve enzyme stability but also reduce the final cost of enzymatic reactions. Herein, we explored the suitability of the amino-functionalized zeolite/SiO2 materials to co-immobilize trypsin-chymotrypsin mixture. For this purpose, the trypsin-chymotrypsin mixture was co-immobilized on the amino-functionalized zeolite/SiO2 materials and the immobilization yield was 80.7 +/- 7.6%. The pre-support and its modifi...
Preparation of Chitosan-Coated Magnetite Nanoparticles and Application for Immobilization of Laccase
Kalkan, Nuzhet Ayca; AKSOY, SERPİL; Aksoy, Eda Ayse; Hasırcı, Nesrin (2012-01-15)
In this study, immobilization of laccase (L) enzyme on magnetite (Fe(3)O(4)) nanoparticles was achieved, so that the immobilized enzyme could be used repeatedly. For this purpose, Fe(3)O(4) nanoparticles were coated and functionalized with chitosan (CS) and laccase from Trametes versicolor was immobilized onto chitosan-coated magnetic nanoparticles (Fe(3)O(4)-CS) by adsorption or covalent binding after activating the hydroxyl groups of chitosan with carbodiimide (EDAC) or cyanuric chloride (CC). For chitosa...
Production and biochemical characterization of polyphenol oxidase from thermomyces lanuginosus
Astarcı, Erhan; Bakır, Ufuk; Ögel, Zümrüt B.; Department of Biotechnology (2003)
Polyphenol oxidases are enzymes that catalyze the oxidation of certain phenolic substrates to quinones in the presence of molecular oxygen. Polyphenol oxidases are widely used in several applications. In food industry, they are used for enhancement of flavor in coffee, tea and cocoa production, and determination of food quality. In medicine, they have several uses in treatments of Parkinson̕s disease, phenlyketonurea and leukemia. In wastewater treatment, they are used for the removal of phenolic pollutants...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
M. OZDEN and V. N. Hasırcı, “PREPARATION AND CHARACTERIZATION OF POLYMER COATED SMALL UNILAMELLAR VESICLES,”
BIOCHIMICA ET BIOPHYSICA ACTA
, pp. 102–108, 1991, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/30517.