A rapid method for detection of genetically modified organisms based on magnetic separation and surface-enhanced Raman scattering

Date

2012-01-01

Author

GÜVEN, BURCU
BOYACI, İSMAİL HAKKI
TAMER, UĞUR
Çalık, Pınar

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

212
views

0
downloads

In this study, a new method combining magnetic separation (MS) and surface-enhanced Raman scattering (SERS) was developed to detect genetically modified organisms (GMOs). An oligonucleotide probe which is specific for 35 S DNA target was immobilized onto gold coated magnetic nanospheres to form oligonucleotide-coated nanoparticles. A self assembled monolayer was formed on gold nanorods using 5,5'-dithiobis (2-nitrobenzoic acid) (DTNB) and the second probe of the 35 S DNA target was immobilized on the activated nanorod surfaces. Probes on the nanoparticles were hybridized with the target oligonucleotide. Optimization parameters for hybridization were investigated by high performance liquid chromatography. Optimum hybridization parameters were determined as: 4 mu M probe concentration, 20 min immobilization time, 30 min hybridization time, 55 degrees C hybridization temperature, 750 mM buffer salt concentration and pH: 7.4. Quantification of the target concentration was performed via SERS spectra of DTNB on the nanorods. The correlation between the target concentration and the SERS signal was found to be linear within the range of 25-100 nM. The analyses were performed with only one hybridization step in 40 min. Real sample analysis was conducted using Bt-176 maize sample. The results showed that the developed MS-SERS assay is capable of detecting GMOs in a rapid and selective manner.

Subject Keywords

Analytical Chemistry, Spectroscopy, Electrochemistry, Biochemistry, Environmental Chemistry

URI

https://hdl.handle.net/11511/40471

Journal

ANALYST

DOI

https://doi.org/10.1039/c1an15629b

Collections

Department of Chemical Engineering, Article

Suggestions

OpenMETU
Core

DETERMINATION OF SELENIUM IN BIOLOGICAL MATRICES USING A KINETIC CATALYTIC METHOD GOKMEN, IG; ABDELQADER, E (Royal Society of Chemistry (RSC), 1994-04-01) A simple and sensitive catalytic spectrophotometric method was developed for the determination of selenium in biological matrices. The method is based on the catalytic effect of selenium on the reaction of Methylene Blue (MB) with sodium sulfide. For a given reaction between MB and sodium sulfide, the change in the MB absorbance with time was monitored, then the time (t) required for completion of the reaction was determined, and t(-1) was calculated. A plot of t(-1) versus selenium concentration constitute...
Comprehensive native glycan profiling with isomer separation and quantitation for the discovery of cancer biomarkers Hua, Serenus; An, Hyun Joo; Özcan Kabasakal, Süreyya; Ro, Grace S.; Soares, Stephanie; DeVere-White, Ralph; Lebrilla, Carlito B. (Royal Society of Chemistry (RSC), 2011-01-01) Glycosylation is highly sensitive to the biochemical environment and has been implicated in many diseases including cancer. Glycan compositional profiling of human serum with mass spectrometry has already identified potential biomarkers for several types of cancer and diseases; however, composition alone does not fully describe glycan stereo-and regioisomeric diversity. The vast structural heterogeneity of glycans presents a formidable analytical challenge. We have developed a method to identify and quantif...
2-DIMENSIONAL ELECTROPHORESIS OF PROTEINS WITH A DIFFERENT APPROACH TO ISOELECTRIC-FOCUSING NAQVI, SMS; OZALP, VC; Öktem, Hüseyin Avni; YUCEL, M (Royal Society of Chemistry (RSC), 1994-06-01) A simple method for the two-dimensional analysis of proteins was developed based on direct extraction of a protein sample from dried/precipitated material into isoelectric focusing (IEF) gel solution. This sample solution is equally useful for sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis and two-dimensional electrophoresis. For the former method this sample solution is applied directly to the gel. For the tatter, sample solution containing a desired amount of proteins is mixed with IEF ge...
DETERMINATION OF SELENIUM IN HUMAN DIETS BY RADIOCHEMICAL NEUTRON-ACTIVATION ANALYSIS ELHALLAQ, YH; GOKMEN, IG; ARAS, NK; Gökmen, Ali (Royal Society of Chemistry (RSC), 1992-03-01) A post-irradiation radiochemical separation technique was tested for the determination of selenium levels in diet samples, collected by using a duplicate portion technique, from both rural and urban population groups in Turkey. The technique involved sample irradiation, acid digestion, selective distillation, precipitation and filtration steps. During the separations it was possible to determine the yield of each sample using a stable selenium carrier. An average chemical yield of 71 +/- 3% was obtained ...
A sandwich-type DNA array platform for detection of GM targets in multiplex assay Cansiz, Sena; Özen, Can; Bayrac, Ceren; Bayrac, A. Tahir; Gül, Fatma; Kavruk, Murat; Yilmaz, Remziye; EYİDOĞAN, FÜSUN; Öktem, Hüseyin Avni (2012-09-01) Over the last decade, array detection has been developed to qualitatively assess the presence of genetically modified organisms (GMOs). To date, DNA array systems have the highest capabilities as a result of GMOs analysis. We describe the construction of an array platform in the sandwich hybridization format for the detection of transgenic promoter of Cauliflower mosaic virus (CaMV; p35S). Sequence-specific signal development has been achieved by a sandwich complex composed of a surface immobilized capture ...

Citation Formats

B. GÜVEN, İ. H. BOYACI, U. TAMER, and P. Çalık, “A rapid method for detection of genetically modified organisms based on magnetic separation and surface-enhanced Raman scattering,” ANALYST, pp. 202–208, 2012, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/40471.