DETERMINATION OF SELENIUM IN BIOLOGICAL MATRICES USING A KINETIC CATALYTIC METHOD

Date

1994-04-01

Author

GOKMEN, IG
ABDELQADER, E

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

211
views

0
downloads

A simple and sensitive catalytic spectrophotometric method was developed for the determination of selenium in biological matrices. The method is based on the catalytic effect of selenium on the reaction of Methylene Blue (MB) with sodium sulfide. For a given reaction between MB and sodium sulfide, the change in the MB absorbance with time was monitored, then the time (t) required for completion of the reaction was determined, and t(-1) was calculated. A plot of t(-1) versus selenium concentration constituted the calibration graph, which was linear in the range 2.5-30 ng ml(-1) selenium. In this study, experimental parameters and the effect of interferences on determinations of selenium were examined. Tetramethylammonium hydroxide digestion was applied to blood, hair and urine samples; it was found to give the best results for urine. Then the catalytic method was applied to urine samples (84.9% recovery).

Subject Keywords

Analytical Chemistry, Spectroscopy, Electrochemistry, Biochemistry, Environmental Chemistry

URI

https://hdl.handle.net/11511/65315

Journal

ANALYST

DOI

https://doi.org/10.1039/an9941900703

Collections

Department of Chemistry, Article

Suggestions

OpenMETU
Core

2-DIMENSIONAL ELECTROPHORESIS OF PROTEINS WITH A DIFFERENT APPROACH TO ISOELECTRIC-FOCUSING NAQVI, SMS; OZALP, VC; Öktem, Hüseyin Avni; YUCEL, M (Royal Society of Chemistry (RSC), 1994-06-01) A simple method for the two-dimensional analysis of proteins was developed based on direct extraction of a protein sample from dried/precipitated material into isoelectric focusing (IEF) gel solution. This sample solution is equally useful for sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis and two-dimensional electrophoresis. For the former method this sample solution is applied directly to the gel. For the tatter, sample solution containing a desired amount of proteins is mixed with IEF ge...
A rapid method for detection of genetically modified organisms based on magnetic separation and surface-enhanced Raman scattering GÜVEN, BURCU; BOYACI, İSMAİL HAKKI; TAMER, UĞUR; Çalık, Pınar (Royal Society of Chemistry (RSC), 2012-01-01) In this study, a new method combining magnetic separation (MS) and surface-enhanced Raman scattering (SERS) was developed to detect genetically modified organisms (GMOs). An oligonucleotide probe which is specific for 35 S DNA target was immobilized onto gold coated magnetic nanospheres to form oligonucleotide-coated nanoparticles. A self assembled monolayer was formed on gold nanorods using 5,5'-dithiobis (2-nitrobenzoic acid) (DTNB) and the second probe of the 35 S DNA target was immobilized on the activa...
DETERMINATION OF SELENIUM IN HUMAN DIETS BY RADIOCHEMICAL NEUTRON-ACTIVATION ANALYSIS ELHALLAQ, YH; GOKMEN, IG; ARAS, NK; Gökmen, Ali (Royal Society of Chemistry (RSC), 1992-03-01) A post-irradiation radiochemical separation technique was tested for the determination of selenium levels in diet samples, collected by using a duplicate portion technique, from both rural and urban population groups in Turkey. The technique involved sample irradiation, acid digestion, selective distillation, precipitation and filtration steps. During the separations it was possible to determine the yield of each sample using a stable selenium carrier. An average chemical yield of 71 +/- 3% was obtained ...
Introduction of solid-phase microextraction as a high-throughput sample preparation tool in laboratory analysis of prohibited substances Boyacı, Ezel; Rodriguez-Lafuente, Angel; Bojko, Barbara; Pawliszyn, Janusz (Elsevier BV, 2014-01-27) A fully automated, high-throughput method based on thin-film solid-phase microextraction (SPME) and liquid chromatography-mass spectrometry was developed for simultaneous quantitative analysis of 110 doping compounds, selected from ten classes and varying in physical and chemical properties. Among four tested extraction phases, C18 blades were chosen, as they provided optimum recoveries and the lowest carryover effect. The SPME method was optimized in terms of extraction pH, ionic strength of the sample, wa...
Determination of Arsenobetaine in Fish Tissue by Species Specific Isotope Dilution LC-LTQ-Orbitrap-MS and Standard Addition LC-ICPMS Yang, Lu; Ding, Jianfu; Maxwell, Paulette; McCooeye, Margaret; Windust, Anthony; Ouerdane, Laurent; Bakirdere, Sezgin; Willie, Scott; Mester, Zoltan (American Chemical Society (ACS), 2011-05-01) An accurate and precise method for the determination of arsenobetaine (AsB, (CH(3))(3)(+)AsCH(2)COO(-)) in fish samples using exact matching species specific isotope dilution (ID) liquid chromatography LTQ:Orbitrap mass spectrometry (LC-LTQ-Orbitrap-MS) and standard addition LC inductively coupled plasma mass spectrometry (LC-ICPMS) is described. Samples were extracted by sonication for 30 min with high purity deionized water. An in-house synthesized (13)C enriched AsB spike was used for species specific ID...

Citation Formats

I. GOKMEN and E. ABDELQADER, “DETERMINATION OF SELENIUM IN BIOLOGICAL MATRICES USING A KINETIC CATALYTIC METHOD,” ANALYST, pp. 703–708, 1994, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/65315.