Bioinspired synthesis of self-assembled calcium phosphate nanocomposites using block copolymer-peptide conjugates

Download

index.pdf

Date

2008-12-01

Author

YUSUFOGLU, Yusuf
HU, Yanyan
KANAPATHIPILLAI, Mathumai
KRAMER, Matthew
Kalay, Yunus Eren
THIYAGARAJAN, P.
AKINC, MÜFİT
SCHMIDT-ROHR, Klaus
MALLAPRAGADA, Surya

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

128
views

135
downloads

Thermoreversibly gelling block copolymers conjugated to hydroxyapatite-nucleating peptides were used to template the growth of inorganic calcium phosphate in aqueous solutions. Nuclear magnetic resonance (NMR). Fourier transform infrared (FTIR), transmission electron microscopy, x-ray diffraction, and small-angle scattering were Used to characterize these samples and confirm that the peptides promoted the growth of hydroxyapatite as the inorganic phase. Three different polymer templates were Used with varying charges oil the polymer chains (nonionic. anionic. and zwitterionic), to investigate the role of charge oil mineralization. All of the polymer-inorganic solutions exhibited thermoreversible gelation above room temperature. Nanocomposite formation was confirmed by solid-state NMR, and Several methods identified the Inorganic component as hydroxyapatite. Small angle x-ray scattering and electron microscopy showed thin, elongated crystallites. Thermogravimetric analysis Showed an inorganic content of 30-45 wt% (based oil the mass of the dried,gel at similar to 200 degrees C) in the various samples. Our work offers routes For bioinspired bottom-up approaches for the development of novel, self-assembling, injectable nanocomposite biomaterials for potential orthopedic applications.

Subject Keywords

TRANSFER RADICAL POLYMERIZATION, CRYSTALLINE CARBONATE APATITE, BIOMIMETIC SYNTHESIS, AQUEOUS-SOLUTIONS, NMR-SPECTROSCOPY, ROOM-TEMPERATURE, HYDROXYAPATITE, MINERALIZATION, BONE, SCAFFOLDS

URI

https://hdl.handle.net/11511/40499

Journal

JOURNAL OF MATERIALS RESEARCH

DOI

https://doi.org/10.1557/jmr.2008.0388

Collections

Department of Metallurgical and Materials Engineering, Article

Suggestions

OpenMETU
Core

Quantification of CaCO3-CaSO3 center dot 0.5H(2)O-CaSO4 center dot 2H(2)O mixtures by FTIR analysis and its ANN model Boke, H; Akkurt, S; Ozdemir, S; Gokturk, EH; Saltık, Emine Nevin (2004-02-01) A new quantitative analysis method for mixtures of calcium carbonate (CaCO3), calcium sulphite hemihydrate (CaSO3.1/2H(2)O) and gypsum (CaSO4.2H(2)O) by FTIR spectroscopy is developed. The method involves the FTIR analysis of powder mixtures of several compositions on KBr disc specimens. Intensities of the resulting absorbance peaks for CaCO3, CaSO3.1/2H(2)O and CaSO4.2H(2)O at 1453, 980, 1146 cm(-1) were used as input data for an artificial neural network (ANN) model, the output being the weight percent co...
Controlled release of vancomycin from biodegradable microcapsules Ozalp, Y; Ozdemir, N; Kocagoz, S; Hasırcı, Vasıf Nejat (2001-01-01) Poly D,L-lactic acid (PLA) and its copolymers with glycolide PLGA 90:10 and 70:30 were polymerized under various conditions to yield polymers in the molecular weight range 12 000-40 000 daltons, as determined by gel permeation chromatography. Vancomycin hydrochloride was the hydrophilic drug of choice for the treatment of methicillin resistant Staphyloccoccal infections. It was microencapsulated in the synthesized polymers using water-oil-water (w/o/w) double emulsion and solvent evaporation. The influence ...
Differential expressions and functions of phosphodiesterase enzymes in different regions of the rat heart DERİCİ, MEHMET KÜRŞAT; SADİ, GÖKHAN; Cenik, Basar; Güray, Nülüfer Tülün; DEMİREL YILMAZ, EMİNE (Elsevier BV, 2019-02-05) Phosphodiesterase enzymes (PDEs) are responsible for the adjustment of cyclic nucleotide levels. Alterations in PDE expressions in different tissues cause conflicts between functional and clinical effects of PDE inhibitors. Therefore, the aim of this study was to investigate the gene and protein expressions and the functional role of PDEs in atrium and ventricle of rat heart The expressions of PDEs were examined in cardiac intact tissues and enzymatically isolated cells. The effects of PDE1-5 inhibitors (vi...
Synthesis of block copolymers by combination of photoinduced and atom transfer radical polymerization routes Erel Göktepe, İrem; Serhatli, Ersin; Yağcı, Yusuf (2002-01-01) Block copolymers of methyl methacrylate (MMA) and styrene (St) were obtained by the combination of two different free radical polymerization processes, namely photoinduced and atom transfer radical polymerization (ATRP) methods. In the first step, hydroxy terminated polymers were prepared by photoinitiated polymerization of MMA in the presence of N,N'-dimethylethanolamine and benzophenone as the initiating system and subsequently converted to bromine ended poly(methyl methacrylate) by the esterification of ...
Covalent immobilization of chloroperoxidase onto magnetic beads: Catalytic properties and stability Bayramoglu, Guelay; Kiralp, Senem; Yilmaz, Meltem; Toppare, Levent Kamil; Arica, M. Yakup (Elsevier BV, 2008-02-15) Amino groups containing magnetic beads were used in covalent immobilization of the enzyme "chloroperoxidase (CPO)" which is one of a few enzymes that can catalyse the peroxide dependent oxidation of a wide spectrum of organic and inorganic compounds. The magnetic poly(glycidylmethacrylate-methylmethacrylate-etbyleneglycol dimethacrylate), magnetic p(GMA-MMA-EGDMA) beads were prepared via suspension polymerization in the presence of ferric ions. The magnetic beads were characterized with scanning electron mi...

Citation Formats

Y. YUSUFOGLU et al., “Bioinspired synthesis of self-assembled calcium phosphate nanocomposites using block copolymer-peptide conjugates,” JOURNAL OF MATERIALS RESEARCH, pp. 3196–3212, 2008, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/40499.