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Structural and Biological Analysis of Mesoporous Lanthanum Doped beta TCP For Potential Use as Bone Graft Material

Motameni, Ali
Dalgıç, Ali Deniz
Keskin, Dilek
Evis, Zafer
In this study, mesoporous particles of beta-tricalcium phosphate (beta TCP, beta Ca-3(PO4)(2)) and lanthanum (La) doped beta TCP were synthesized using wet precipitation method attached with microwave refluxing system. The obtained materials were characterized and analysed using different sort of techniques such; X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscope (SEM), Inductively coupled plasma optical emission spectroscopy (ICP-OES), Brunauer-Emmett-Teller (BET) and helium pycnometer. With incorporation of La3+ ions, relevant expansion in lattice parameters of beta TCP crystal was observed along with inhibition growth rate was observed. beta TCP and La-beta TCP materials were mesoporous in nature, pore diameter and pore volume were expanded with incorporation of La3+ ions. The loading/release on beta TCP and La-beta TCP particles surfaces were evaluated using fetal bovine serum (FBS) proteins. In vitro cell culture studies were performed with human osteosarcoma cell line (Saos-2). The outcomes approved that all La-beta TCP materials were cytocompatible and strict dose dependent effect of La3+ ions was observed on cell viability and alkaline phosphatase (ALP) activity. These results strongly suggest that La-beta TCP materials have a potential application in bone tissue engineering.