Metal oxide surfaces for enhanced colorimetric response in bioassays

Date

2017-06-01

Author

Bonyi, Enock
Kukoyi, Zeenat
Daodu, Oluseyi
Boone-Kukoyi, Zainab
COŞKUN, Sahin
Ünalan, Hüsnü Emrah
Asian, Kadir

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

108
views

0
downloads

Physical stability of metal nanoparticle films on planar surfaces can be increased by employing surface modification techniques and/or type of metal nanoparticles. Subsequently, the enzymatic response of colorimetric bioassays can be increased for improved dynamic range for the detection of biomolecules. Using a model bioassay b-BSA, three planar platforms (1) poly (methyl methacrylate) (PMMA) with silver thin films (STFs), (2) silver nanowires (Ag NWs) on paper and (3) indium tin oxide (ITO) on polyethylene terephthalate (PET) were evaluated to investigate the extent of increase in the colorimetric signal. Bioassays for b-BSA and Ki-67 antigen (a real-life bioassay) in buffer were performed using microwave heating (total assay time is 25-30 min) and at room temperature (a control experiment, total assay time is 3 h). Model bioassays showed that STFs were removed from the surface during washing steps and the extent of ITO remained unchanged. The lowest level of detection (LLOD) for b-BSA bioassays were: 10(-10) M for 10 nm STFs on PMMA and Ag NWs on paper and 10(-11) M for ITO. Bioassays for Ki-67 antigen yielded a LLOD of <10(-9) Mon ITO platforms, while STFs platforms were deemed unusable due to significant loss of STFs from the surfaces.

Subject Keywords

Biotechnology, Physical and Theoretical Chemistry, Colloid and Surface Chemistry, Surfaces and Interfaces, General Medicine

URI

https://hdl.handle.net/11511/40708

Journal

COLLOIDS AND SURFACES B-BIOINTERFACES

DOI

https://doi.org/10.1016/j.colsurfb.2017.03.030

Collections

Department of Metallurgical and Materials Engineering, Article

Suggestions

OpenMETU
Core

Metallization of the C-60/Rh(100) interface revealed by valence photoelectron spectroscopy and density functional theory calculations Wade, Abdou-Ciss; Lizzit, Silvano; Petaccia, Luca; Goldoni, Andrea; Diop, Djibril; Toffoli, Hande; Fabris, Stefano; Baroni, Stefano (AIP Publishing, 2010-06-01) The electronic structure of single and multiple layers of C-60 molecules deposited on a Rh(100) surface is investigated by means of valence photoemission spectroscopy and density functional theory calculations. The binding of the fullerene monolayer to the metal surface yields the appearance of a new state in the valence band spectrum crossing the Fermi level. Insight into the metallization of the metal/fullerene interface is provided by the calculated electronic structure that allows us to correlate the me...
Indium tin oxide nanoparticles as anode for light-emitting diodes Çırpan, Ali (Wiley, 2006-02-15) Thin films of indium tin oxide (ITO) nanoparticles have been investigated as anode materials for polymer light-emitting diodes. A luminance efficiency (0.13 cd/ A), higher than that (0.09 cd/A) obtained in a control devices fabricated on conventional commercial ITO anodes were found. The thin films were made by spin coating of a suspension followed by annealing. The ITO nartoparticle films have a stable sheet resistance of 200 ohm/sq, and an optical transmittance greater than 86% over the range of 400-1000 ...
Flexible organic photovoltaics from zinc oxide nanowires grown on transparent and conducting single walled carbon nanotube thin films Ünalan, Hüsnü Emrah; Kuo, Daniel; Parekh, Bhavin; Amaratunga, Gehan; Chhowalla, Manish (Royal Society of Chemistry (RSC), 2008-01-01) The fabrication of flexible organic photovoltaics (OPVs) which utilize transparent and conducting single walled carbon nanotube (SWNT) thin films as current collecting electrodes on plastic substrates in zinc oxide nanowire (ZnO NW)/poly(3-hexylthiophene) (P3HT) bulk heterojunction photovoltaic devices is reported. The bulk heterojunctions for exciton dissociation are created by directly growing ZnO nanowires from solution on the SWNT electrodes and spin coating the P3HT polymer. A maximum OPV power convers...
Poly(methyl methacrylate) hybrid syntactic foams with hollow glass microspheres and polyhedral oligomeric silsesquioxanes Ozkutlu, Merve; Dilek Hacıhabiboğlu, Çerağ; Bayram, Göknur (Wiley, 2019-08-10) The study aims to produce poly(methyl methacrylate) (PMMA)-based lower density syntactic foams with hollow glass microspheres (HGMs) and to improve their mechanical properties by the addition of polyhedral oligomeric silsesquioxanes (POSSs) while maintaining the thermal properties of the neat polymer. First to understand the effect of POSS addition, PMMA-POSS composites with octaisobutyl and octaphenyl POSS were produced through extrusion. Higher relative flexural and impact strengths were obtained with POS...
Bond strength of a silicone soft lining material to poly(methyl methacrylate) resin treated with maleic anhydride and its terpolymers Turgut, Mehmet; Bolayir, Giray; Dogan, Orhan Murat; Keskin, Selda; Dogan, Arife; Boztug, Ali (Informa UK Limited, 2007-01-01) This study investigated the effectiveness of surface treatment of Poly (methyl methacrylate) (PMMA) denture base resin on tensile bond strength between PMMA/silicone-based soft liner. A total of 25 specimens were fabricated and assigned into five groups (n = 5). The surfaces of PMMA were treated with maleic anhydride, maleic anhydride-styrene-vinyl-acetate, n-butylmaleate-styrene-vinyl-acetate, or n-pentamaleate-styrene-vinyl-acetate prior to Primo adhesive primer application and silicone liner placement. T...

Citation Formats

E. Bonyi et al., “Metal oxide surfaces for enhanced colorimetric response in bioassays,” COLLOIDS AND SURFACES B-BIOINTERFACES, pp. 331–340, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/40708.