ALCAM is indirectly modulated by miR-125b in MCF7 cells

Date

2015-05-01

Author

AKMAN, H. Begum
SELCUKLU, S. Duygu
DONOGHUE, Mark T. A.
AKHAVANTABASI, Shiva
SAPMAZ, Aysegul
SPILLANE, Charles
Yakicier, M. Cengiz
Erson Bensan, Ayşe Elif

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MicroRNA (miRNA) deregulation is associated with various cancers. Among an expanding list of cancer-related miRNAs, deregulation of miR-125b has been well documented in many cancers including breast. Based on current knowledge, miR-125b is considered to be a tumor suppressor in breast cancers. While important messenger RNA (mRNA) targets have been defined for miR-125b, here, we aimed to further investigate direct/indirect consequences of miR-125b expression in breast cancer cells by using a transcriptome approach. Upon miR-125b expression, a total of 138 cancer-related genes were found to be differentially expressed in breast cancer cells. While only a few of these were predicted to be direct mRNA targets, majority of the gene expression changes were potentially downstream and indirect effects of miR-125b expression. Among these, activated leukocyte antigen molecule (ALCAM) mRNA and protein levels were found to be highly significantly increased upon miR-125b expression. Given the tumor suppressor role of miR-125b in our model system, upon silencing of ALCAM expression, cell proliferation rate re-increased in miR-125b-expressing cells. While ALCAM's possible context-dependent roles are not clear in breast cancer, a diverse expression pattern of ALCAM mRNA was detected in a panel of breast cancer patient samples. Differentially expressed/regulated cancer-related genes upon miR-125b expression along with the significant increase of ALCAM are of future interest to understand how deregulated expression of miR-125b may have a tumor suppressor role in breast and other cancers.

Subject Keywords

miR-125b, ALCAM, Breast cancer, PERP, CTGF, Microarray

URI

https://hdl.handle.net/11511/40738

Collections

Department of Biology, Article