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ALCAM is indirectly modulated by miR-125b in MCF7 cells
Date
2015-05-01
Author
AKMAN, H. Begum
SELCUKLU, S. Duygu
DONOGHUE, Mark T. A.
AKHAVANTABASI, Shiva
SAPMAZ, Aysegul
SPILLANE, Charles
Yakicier, M. Cengiz
Erson Bensan, Ayşe Elif
Metadata
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This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
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MicroRNA (miRNA) deregulation is associated with various cancers. Among an expanding list of cancer-related miRNAs, deregulation of miR-125b has been well documented in many cancers including breast. Based on current knowledge, miR-125b is considered to be a tumor suppressor in breast cancers. While important messenger RNA (mRNA) targets have been defined for miR-125b, here, we aimed to further investigate direct/indirect consequences of miR-125b expression in breast cancer cells by using a transcriptome approach. Upon miR-125b expression, a total of 138 cancer-related genes were found to be differentially expressed in breast cancer cells. While only a few of these were predicted to be direct mRNA targets, majority of the gene expression changes were potentially downstream and indirect effects of miR-125b expression. Among these, activated leukocyte antigen molecule (ALCAM) mRNA and protein levels were found to be highly significantly increased upon miR-125b expression. Given the tumor suppressor role of miR-125b in our model system, upon silencing of ALCAM expression, cell proliferation rate re-increased in miR-125b-expressing cells. While ALCAM's possible context-dependent roles are not clear in breast cancer, a diverse expression pattern of ALCAM mRNA was detected in a panel of breast cancer patient samples. Differentially expressed/regulated cancer-related genes upon miR-125b expression along with the significant increase of ALCAM are of future interest to understand how deregulated expression of miR-125b may have a tumor suppressor role in breast and other cancers.
Subject Keywords
miR-125b
,
ALCAM
,
Breast cancer
,
PERP
,
CTGF
,
Microarray
URI
https://hdl.handle.net/11511/40738
Journal
TUMOR BIOLOGY
DOI
https://doi.org/10.1007/s13277-014-2987-5
Collections
Department of Biology, Article