Physicochemical mechanisms of different biopolymers' (lysozyme, gum arabic, whey protein, chitosan) adsorption on green tea extract loaded liposomes

Date

2019-10-01

Author

Dag, Damla
Güner, Selen
Öztop, Halil Mecit

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

155
views

0
downloads

Having various domains of applicability, liposomes have been the issue of many studies since 1960s. Kinetically stable nature of liposomes required incorporation of other substituents to gain storage stability and interaction of liposomes with polymers, electrolytes, proteins or lipids still requires further investigation to explain the underlying mechanism. In this study, polyphenol-rich green tea extract was encapsulated into liposomes by means of microfluidization in two different aqueous media (pH = 3.8 acetate buffer and pH = 6.5 distilled water). Antioxidant loaded vesicles were further mixed with anionic biopolymers (gum arabic, whey protein) and cationic biopolymers (lysozyme, chitosan) separately. The physical and chemical interactions between liposomes and biopolymers were rationalized by particle size, zeta potential, transmission electron microscopy, total phenolic content and antioxidant activity measurements during 28-days storage at 4 degrees C. Experimental results indicated that the biopolymer incorporated liposomes showed better stability compared to control liposomes during storage, developing resistance against changes in particle size and zeta potential. On the other hand, biopolymer interaction mechanisms were shown to be different for different biopolymers. As was also proved by transmission electron microscopy, lysozyme was absorbed into the liposomes while gum arabic, whey protein and chitosan were adsorbed on the vesicle surface to shield green tea extract loaded liposomes

Subject Keywords

Biochemistry, Molecular Biology, Structural Biology, General Medicine

URI

https://hdl.handle.net/11511/41506

Journal

International Journal of Biological Macromolecules

DOI

https://doi.org/10.1016/j.ijbiomac.2019.07.106

Collections

Department of Food Engineering, Article

Suggestions

OpenMETU
Core

Discovering functional interaction patterns in protein-protein interaction networks Turanalp, Mehmet E.; Can, Tolga (Springer Science and Business Media LLC, 2008-06-11) Background: In recent years, a considerable amount of research effort has been directed to the analysis of biological networks with the availability of genome-scale networks of genes and/or proteins of an increasing number of organisms. A protein-protein interaction (PPI) network is a particular biological network which represents physical interactions between pairs of proteins of an organism. Major research on PPI networks has focused on understanding the topological organization of PPI networks, evolution...
An alternative supporting electrolyte for enzyme immobilization in conducting polymers Kiralp, Senem; Balik, Balam; KARATAŞ, SEVİM; Toppare, Levent Kamil; Gungorb, Atilla (Elsevier BV, 2008-03-01) In this study an alternative supporting electrolyte was used in enzyme immobilization. Invertase was studied to observe the effect of the supporting electrolyte. Sulfonated poly(arylene ether sulfone) was used as the supporting electrolyte during the electrolysis of pyrrole. The results show that the polymeric supporting electrolyte can be used instead of sodium dodecyl sulfate.
Various applications of immobilized glucose oxidase and polyphenol oxidase in a conducting polymer matrix Cil, M.; Boyukbayram, A. E.; Kiralp, S.; Toppare, Levent Kamil; Yagci, Y. (Elsevier BV, 2007-06-01) In this study, glucose oxidase and polyphenol oxidase were immobilized in conducting polymer matrices; polypyrrole and poly(N-(4-(3-thienyl methylene)-oxycarbonyl phenyl) maleimide-co-pyrrole) via electrochemical method. Fourier transform infrared and scanning electron microscope were employed to characterize the copolymer of (N-(4-(3-thienyl methylene)-oxycarbonyl phenyl) maleimide) with pyrrole. Kinetic parameters, maximum reaction rate and Michealis-Menten constant, were determined. Effects of temperatur...
Optimization of selection conditions and agrobacterium mediated transformation of chickpea (cicer arietinum L. cv. Gökçe) Öz, M. Tufan; Öktem, Hüseyin Avni; Department of Biotechnology (2005) The objective of this study was to optimize an efficient selection system and Agrobacterium mediated transformation of chickpea (Cicer arietinum L.). Cotyledonary node explants of Turkish chickpea cultivar Gökçe were used to determine the effects of selective agents, two antibiotics (Kanamycin, Hygromycin) and two herbicides (PPT, Glyphosate) as well as four antibiotics (Augmentin, Carbenicillin, Cefotaxime, Timentin) for eliminating Agrobacterium on multiple shoot and root induction. Selective agents and a...
Inference of switching networks by using a piecewise linear formulation Akçay, Didem; Öktem, Hakan; Department of Scientific Computing (2005) Inference of regulatory networks has received attention of researchers from many fields. The challenge offered by this problem is its being a typical modeling problem under insufficient information about the process. Hence, we need to derive the apriori unavailable information from the empirical observations. Modeling by inference consists of selecting or defining the most appropriate model structure and inferring the parameters. An appropriate model structure should have the following properties. The model...

Citation Formats

D. Dag, S. Güner, and H. M. Öztop, “Physicochemical mechanisms of different biopolymers’ (lysozyme, gum arabic, whey protein, chitosan) adsorption on green tea extract loaded liposomes,” International Journal of Biological Macromolecules, pp. 473–482, 2019, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/41506.