Production of L-aspartic acid by biotransformation and recovery using reverse micelle and gas hydrate methods

Date

2007-01-01

Author

Aydogan, Oezlem
Bayraktar, Emine
Parlaktuna, Mahmut
Mehmetoglu, Tanju
Mehmetoglu, Uelkue

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

217
views

0
downloads

L-Aspartic acid (L-Asp) was produced using Escherichia coli (ATCC 11303), and its recovery from the reaction mixture was studied using reverse micelle and gas hydrate methods. The effect of initial substrate concentration on L-Asp production was also investigated, and inhibition was shown to occur above 0.75 mol L-1. The values of the kinetic constants were determined as r(max) = 2.33 x 10(-4) mol L-1 min(-1), K-M = 0.19 mol L-1, and K-ss = 3.98 mol L-1. The reverse micelle phase used for extraction contained Aliquat-336, 1-decanol and isooctane, and a micro-injection technique was used for extraction of L-Asp. The reverse micelle system is a useful technique for obtaining small particle sizes, which can be used for the synthesis of nanoparticle biomolecules. Recovery of L-Asp from reverse micelles using CO2 hydrates was carried out, giving a recovery of 55%. The formation of CO2 hydrate from the reverse micelle solution breaks the micelle by reducing the amount of water in the micelle structure, thus precipitating the L- Asp.

Subject Keywords

Biotechnology, Biochemistry, Catalysis

URI

https://hdl.handle.net/11511/41622

Journal

BIOCATALYSIS AND BIOTRANSFORMATION

DOI

https://doi.org/10.1080/10242420701510395

Collections

Department of Petroleum and Natural Gas Engineering, Article

Suggestions

OpenMETU
Core

Preparation of cross-linked tyrosinase aggregates Aytar, Burcu Selin; Bakir, Ufuk (Elsevier BV, 2008-02-01) Tyrosinase from mushroom was immobilized as a cross-linked enzyme aggregate (CLEA) via precipitation with ammonium sulfate and cross-linking with glutaraldehyde. The effects of precipitation and cross-linking on CLEA activity were investigated and the immobilized tyrosinase was characterized. Sixty percent ammonium sulfate saturation and 2% glutaraldehyde were used; a 3-h cross-linking reaction at room temperature, at pH 7.0 was performed; particle sizes of the aggregates were reduced; consequently, 100% ac...
Kinetic analyses of the effects of temperature and light intensity on growth, hydrogenm production and organic acid utilization by rhodobacter capsulatus Sevinç, Pelin; Gündüz, Ufuk; Department of Biotechnology (2010) Effects of temperature and light intensity on photofermentative hydrogen production by Rhodobacter capsulatus DSM1710 by use of acetic and lactic acids as substrates were studied. Experiments were conducted at 20, 30 and 38oC incubator temperatures under light intensities in the 1500 – 7000 lux range. pH of the medium and quantity of hydrogen forming together with quantity of biomass, and concentrations of acetic, lactic, formic, butyric and propionic acids in the medium were determined periodically. Growth...
Degradation of chlorinated compounds by Penicillium camemberti in batch and up-flow column reactors Taseli, BK; Gökçay, Celal Ferdi (Elsevier BV, 2005-02-01) A Penicillium camemberti strain isolated in this laboratory was studied for its ability to degrade chlorinated compounds including pentachlorophenol (PCP), 2-chlorophenol and trichloroacetic acid. The batch experiments were conducted in shake flasks using PCP as co-substrate resulting in around 56% PCP removal by the fungus. Experiments in shake flasks not containing acetate but Tween 80, produced 86% of PCP and 53% of 2-chlorophenol removals in 21 days. PCP was also fed to a column reactor with an adsorbab...
Influence of oxygen transfer on benzaldehyde lyase production by recombinant Escherichia coli BL21(DE3) pLySs Angardi, Vahideh; Çalık, Pınar; Department of Chemical Engineering (2007) In this study, the effects of oxygen transfer conditions on the synthesis of the enzyme benzaldehyde lyase as intracellular in recombinant E. coli BL21 (DE3) pLysS was investigated sistematically and a comprehensive model was developed to determine benzaldehyde lyase activity. For this purpose, the research program was carried out in mainly two parts. In the first part of study, the effects of oxygen transfer together with the mass transfer coefficient (KLa), enhancement factor E (=KLa/KLao), volumetric oxy...
Salt, EDTA, and pH effects on rheological behavior of mold suspensions Dik, Tunay; Bozoğlu, T. Faruk (Elsevier BV, 1992-11) The effects of surface properties on the rheological behavior of Aspergillus niger suspensions were studied at pH 2-7, with the addition of 0-0.10 m NaH2PO4, NaCl, CaCl2, AlCl3, and EDTA at shear rates of 0-21.6 s-1. The structural network concept was used to discuss the consequences of cell-cell interactions on the rheological behavior. Analysis of the data indicated that the suspensions containing CaCl2 nearly always had the smallest shear stress at all the pH values, indicating that, unlike with the yeas...

Citation Formats

O. Aydogan, E. Bayraktar, M. Parlaktuna, T. Mehmetoglu, and U. Mehmetoglu, “Production of L-aspartic acid by biotransformation and recovery using reverse micelle and gas hydrate methods,” BIOCATALYSIS AND BIOTRANSFORMATION, pp. 365–372, 2007, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/41622.