Immobilization of Tyrosinase in Poly(2-thiophen-3-yl-alkyl ester) Derivatives

Date

2008-01-01

Author

ÇAMURLU, PINAR
Kayahan, Senem
Toppare, Levent Kamil

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

172
views

0
downloads

In this study, construction of novel biosensors for the determination of phenolic compound was performed via immobilization of tyrosinase during the electrochemical synthesis of conducting block copolymers of 2-thiophen-3-yl-alkyl ester derivatives with 3,4-ethylenedioxythiophene and synthesis of poly(3,4-ethylenedioxythiophene) (PEDOT). The resultant biosensors were characterized in terms of their maximum reaction rates, Michaelis-Menten constants (Km), temperature and pH stabilities. All the copolymer matrices represented higher reaction rates and higher Km values in comparison to both polypyrrole and PEDOT matrices and a relation between the morphology of the matrice and the kinetic parameters was observed. Biosensors maintained their activity even at temperatures as high as 80C and could be used at pHs higher than 8 with high precision. The amount of phenolics in actual samples (red wines) was investigated using electrodes, and results were compared with those found from Folin-Ciocalteau method. Hence, the present study has proven the suitability of these copolymers to be used as polymer matrices for enzyme immobilization.

Subject Keywords

Materials Chemistry, General Chemistry, Polymers and Plastics, Ceramics and Composites

URI

https://hdl.handle.net/11511/41649

Journal

JOURNAL OF MACROMOLECULAR SCIENCE PART A-PURE AND APPLIED CHEMISTRY

DOI

https://doi.org/10.1080/10601320802454169

Collections

Department of Chemistry, Article

Suggestions

OpenMETU
Core

Reactive extrusion of poly(ethylene terephthalate)-(ethylene/methyl acrylate/glycidyl methacrylate)-organoclay nanocomposites Alyamac, Elif; Yılmazer, Ülkü (Wiley, 2007-04-01) This study was conducted to investigate the effects of component concentrations and addition order of the components on the final properties of ternary nanocomposites composed of poly(ethylene terephthalate), organoclay, and an ethylene-methyl acrylate-glycidyl methacrylate (E-MA-GMA) terpolymer acting as an impact modifier for PET. In this context, first, the optimum amount of the impact modifier was determined by melt compounding binary PET-terpolymer blends in a corotating twin-screw extruder. The amount...
Immobilization of Invertase in Copolymer of 2,5-Di(thiophen-2-yl)-1-p-Tolyl-1H-Pyrrole with Pyrrole Celebi, Selin; Ibibikcan, Esin; Kayahan, Senem; Yigitsoy, Basak; Toppare, Levent Kamil (Informa UK Limited, 2009-01-01) Immobilization of invertase in conducting copolymer matrix of 2,5-di(thiophen-2-yl)-1-p-tolyl-1H-pyrrole with pyrrole (poly(DDTP-co-Py)) was achieved via electrochemical polymerization. Kinetic parameters, Michaelis-Menten constant, Km and the maximum reaction rate, Vmax were investigated. Operational stability and temperature optimization of the enzyme electrodes were also examined. Immobilized invertase reveals maximum activity at 50 degrees C and; pH 8 and pH 4 for two copolymer matrices. Although the sa...
Degradation of Poly(2-hydroxyethyl methacrylate) Obtained by Radiation in Aqueous Solution Varguen, Elif; Usanmaz, Ali (Informa UK Limited, 2010-01-01) The degradation of poly(hydroxyethyl methacrylate), PHEMA obtained by -radiation induced polymerization of HEMA in aqueous solution, was studied. The polymer was a gel type and insoluble in common organic solvents. The DSC thermogram of the polymer gave a Tg value at 88.2 degrees C and an endothermic peak showed further polymerization or crosslinking at 110-160 degrees C. The degradation observed in TGA was a depolymerization type. However, the FT-IR of TGA fragments showed no monomer, which was degraded fu...
Immobilization of invertase and glucose oxidase in poly 2-methylbutyl-2-(3-thienyl) acetate/polypyrrole matrices Isik, S; Alkan, S; Toppare, Levent Kamil; Cianga, I; Yagci, Y (Elsevier BV, 2003-12-01) Immobilization of invertase and glucose oxidase in conducting polypyrrole and copolymers of poly 2-methylbutyl-2-(3-thienyl) acetate with pyrrole were achieved via electrochemical method. Sodium dodecyl sulphate was found to be the most suitable supporting electrolyte. Maximum reaction rate, Michaelis-Menten constant and optimum temperatures were determined for native and immobilized enzymes. Storage and operational stabilities of enzyme electrodes were also investigated.
Thiophene ended epsilon-caprolactone conducting copolymers and their electrochromic properties Kerman, I; Toppare, Levent Kamil; Yilmaz, F; Yagci, Y (Informa UK Limited, 2005-04-01) epsilon-Caprolactone was polymerized by ring-opening polymerization (ROP), using thiophene methanol as the initiator and stannous octoate as the catalyst to yield poly(epsilon-caprolactone) with a thiophene end group. Homopolymerization of thiophene functionalized poly (epsilon-caprolactone) (PCL) was achieved by a constant current electrolysis method. Copolymerizations of PCL with thiophene and pyrrole were achieved in acetonitrile (ACN)-tetrabutylammonium tetrafluoroborate (TBAFB) solvent-electrolyte coup...

Citation Formats

P. ÇAMURLU, S. Kayahan, and L. K. Toppare, “Immobilization of Tyrosinase in Poly(2-thiophen-3-yl-alkyl ester) Derivatives,” JOURNAL OF MACROMOLECULAR SCIENCE PART A-PURE AND APPLIED CHEMISTRY, pp. 1011–1016, 2008, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/41649.