Immobilization of Tyrosinase in Poly(2-thiophen-3-yl-alkyl ester) Derivatives

Date

2008-01-01

Author

ÇAMURLU, PINAR
Kayahan, Senem
Toppare, Levent Kamil

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In this study, construction of novel biosensors for the determination of phenolic compound was performed via immobilization of tyrosinase during the electrochemical synthesis of conducting block copolymers of 2-thiophen-3-yl-alkyl ester derivatives with 3,4-ethylenedioxythiophene and synthesis of poly(3,4-ethylenedioxythiophene) (PEDOT). The resultant biosensors were characterized in terms of their maximum reaction rates, Michaelis-Menten constants (Km), temperature and pH stabilities. All the copolymer matrices represented higher reaction rates and higher Km values in comparison to both polypyrrole and PEDOT matrices and a relation between the morphology of the matrice and the kinetic parameters was observed. Biosensors maintained their activity even at temperatures as high as 80C and could be used at pHs higher than 8 with high precision. The amount of phenolics in actual samples (red wines) was investigated using electrodes, and results were compared with those found from Folin-Ciocalteau method. Hence, the present study has proven the suitability of these copolymers to be used as polymer matrices for enzyme immobilization.

Subject Keywords

Materials Chemistry, General Chemistry, Polymers and Plastics, Ceramics and Composites

URI

https://hdl.handle.net/11511/41649

Journal

JOURNAL OF MACROMOLECULAR SCIENCE PART A-PURE AND APPLIED CHEMISTRY

DOI

https://doi.org/10.1080/10601320802454169

Collections

Department of Chemistry, Article

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Citation Formats

P. ÇAMURLU, S. Kayahan, and L. K. Toppare, “Immobilization of Tyrosinase in Poly(2-thiophen-3-yl-alkyl ester) Derivatives,” JOURNAL OF MACROMOLECULAR SCIENCE PART A-PURE AND APPLIED CHEMISTRY, pp. 1011–1016, 2008, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/41649.