Immobilization of invertase and glucose oxidase in poly 2-methylbutyl-2-(3-thienyl) acetate/polypyrrole matrices

Isik, S
Alkan, S
Toppare, Levent Kamil
Cianga, I
Yagci, Y
Immobilization of invertase and glucose oxidase in conducting polypyrrole and copolymers of poly 2-methylbutyl-2-(3-thienyl) acetate with pyrrole were achieved via electrochemical method. Sodium dodecyl sulphate was found to be the most suitable supporting electrolyte. Maximum reaction rate, Michaelis-Menten constant and optimum temperatures were determined for native and immobilized enzymes. Storage and operational stabilities of enzyme electrodes were also investigated.


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Electrochemical polymerisation of 2-aminofluorene, 2AF, was investigated in ethylalcohol/water mixture (3:2, v:v) in the presence of HClO4 as the supporting electrolyte via constant potential electrolysis, CPE. Prior to CPE, electrochemical behaviour of the monomer was investigated in the same solvent electrolyte couple utilising cyclic voltammetry, CV. Electrochemical polymerisation of the monomer yielded insoluble, dark bluish-green, conducting polymer deposit on the electrode surface. Characterisation of...
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In this study, immobilizations of invertase and glucose oxidase were achieved in conducting thiophene functionalized copolymers of vinyl alcohol with thiophene side groups and pyrrole (PVATh/PPy) via electrochemical polymerization. The kinetic parameters, V-max (maximum reaction rate) and K-m (substrate affinity), of both free and immobilized enzymes were determined. The effect of supporting electrolytes, p-toluene sulfonic acid and sodium dodecyl sulfate, on the enzyme activity and film morphologies was ex...
KISAKUREK, D; YIGIT, S (Elsevier BV, 1991-01-01)
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Immobilization of Invertase in Copolymer of 2,5-Di(thiophen-2-yl)-1-p-Tolyl-1H-Pyrrole with Pyrrole
Celebi, Selin; Ibibikcan, Esin; Kayahan, Senem; Yigitsoy, Basak; Toppare, Levent Kamil (Informa UK Limited, 2009-01-01)
Immobilization of invertase in conducting copolymer matrix of 2,5-di(thiophen-2-yl)-1-p-tolyl-1H-pyrrole with pyrrole (poly(DDTP-co-Py)) was achieved via electrochemical polymerization. Kinetic parameters, Michaelis-Menten constant, Km and the maximum reaction rate, Vmax were investigated. Operational stability and temperature optimization of the enzyme electrodes were also examined. Immobilized invertase reveals maximum activity at 50 degrees C and; pH 8 and pH 4 for two copolymer matrices. Although the sa...
Citation Formats
S. Isik, S. Alkan, L. K. Toppare, I. Cianga, and Y. Yagci, “Immobilization of invertase and glucose oxidase in poly 2-methylbutyl-2-(3-thienyl) acetate/polypyrrole matrices,” EUROPEAN POLYMER JOURNAL, pp. 2375–2381, 2003, Accessed: 00, 2020. [Online]. Available: