Porous clinoptilolite-nano biphasic calcium phosphate scaffolds loaded with human dental pulp stem cells for load bearing orthopedic applications

Alshemary, Ammar Zeıdan Ghaılan
Pazarçeviren, Ahmet Engin
Keskin, Dilek
Tezcaner, Ayşen
Hussain, Rafaqat
Evis, Zafer
Clinoptilolite (Cpt)-nanohydroxyapatite (HA) (Cpt-HA) scaffolds were fabricated as a potential material for load bearing orthopaedic applications. Cpt-HA materials were successfully synthesized by using microwave assisted reflux method followed by the fabrication of three-dimensional (3D) porous scaffold via thermal decomposition process using polyethylene glycol (PEG)/polyvinyl alcohol (PVA) as porogens. The scaffold materials were characterized using x-ray diffraction, Fourier transform Infra-red, Scanning electron microscopy and Energy dispersive spectroscopy techniques. Incorporation of Cpt in HA scaffold significantly increased the compressive strength and surface hardness while scaffolds retained an interconnected porous structure with 64% porosity. Human dental pulp stem cells (DPSCs) were isolated from the third molar and used as pluripotent-like cell model to evaluate the biological properties of Cpt-HA scaffolds. Highest cellular attachment and proliferation were observed for DPSCs seeded on 2.0 g Cpt-HA scaffolds compare to pure HA. Similarly, significantly higher ALP activity of cells was observed on Cpt-HA scaffolds compared to pure HA. The enhanced proliferation and osteogenic response of the DPSCs cultured on Cpt-HA scaffolds suggest that the fabricated scaffolds can be used in bone tissue engineering. In this work, we have successfully shown that the interconnected porous Cpt-HA scaffolds have superior mechanical biological properties compared to pure HA scaffold.


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Native extracellular matrix/fibroin hydrogels for adipose tissue engineering with enhanced vascularization
Kayabolen, Alisan; Keskin, Dilek; Aykan, Andac; Karslioglu, Yildirim; Zor, Fatih; Tezcaner, Ayşen (IOP Publishing, 2017-06-23)
Adipose tissue engineering is a promising field for regeneration of soft tissue defects. However, vascularization is needed since nutrients and oxygen cannot reach cells in thick implants by diffusion. Obtaining a biocompatible scaffold with good mechanical properties is another problem. In this study, we aimed to develop thick and vascularized adipose tissue constructs supporting cell viability and adipose tissue regeneration. Hydrogels were prepared by mixing rat decellularized adipose tissue (DAT) and si...
Fatigue behavior of TiNi foams processed by the magnesium space holder technique
Nakas, G. Ipek; Dericioğlu, Arcan Fehmi; Bor, Sakir (Elsevier BV, 2011-11-01)
While the wide range of applications of TiNi alloys makes them highly appealing due to their shape memory and superelasticity properties, the production of TiNi in the porous form further enlarges their application fields. Porous TiNi alloys have been studied extensively for biomedical applications since their elastic modulus is similar to that of bone. Accordingly, TiNi foams have been widely characterized in terms of their various mechanical properties; however, their fatigue properties have not been well...
Biodegradable polymer promotes osteogenic differentiation in immortalized and primary osteoblast-like cells
Onat, Bora; Tuncer, Sinem; Ulusan, Sinem; Banerjee, Sreeparna; Erel Göktepe, İrem (IOP Publishing, 2019-07-01)
Biodegradable polymers have been broadly used as agents that can complex with and deliver osteoinductive agents, but osteoinductivity of the polymers themselves has been rarely studied. Here we report the osteoinductivity of poly(4-hydroxy-L-proline ester) (PHPE), a biodegradable cationic polymer with cell penetrating properties. Under physiological conditions, PHPE degrades into trans-4-hydroxy-L-prohne (trans-Hyp), a non-coded amino acid with essential functions in collagen fibril formation and fibril sta...
Composite clinoptilolite/PCL-PEG-PCL scaffolds for bone regeneration: In vitro and in vivo evaluation
Pazarçeviren, Ahmet Engin; Altunbas, Korhan; Yaprakci, Volkan; Erdemli, Ozge; Keskin, Dilek; Tezcaner, Ayşen (Wiley, 2020-01-01)
In this study, clinoptilolite (CLN) was employed as a reinforcement in a polymer-based composite scaffold in bone tissue engineering and evaluated in vivo for the first time. Highly porous, mechanically stable, and osteogenic CLN/PCL-PEG-PCL (CLN/PCEC) scaffolds were fabricated with modified particulate leaching/compression molding technique with varying CLN contents. We hypothesized that CLN reinforcement in a composite scaffold will improve bone regeneration and promote repair. Therefore, the scaffolds we...
Citation Formats
A. Z. G. Alshemary, A. E. Pazarçeviren, D. Keskin, A. Tezcaner, R. Hussain, and Z. Evis, “Porous clinoptilolite-nano biphasic calcium phosphate scaffolds loaded with human dental pulp stem cells for load bearing orthopedic applications,” BIOMEDICAL MATERIALS, pp. 0–0, 2019, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/41732.