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A smartphone based surface plasmon resonance imaging (SPRi) platform for on-site biodetection

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2017-02-01
Güner, Havva Gizem
Ozgur, Erol
Kokturk, Guzin
Celik, Mehmet
Esen, Elif
Topal, Ahmet E.
Ayas, Sencer
Uludag, Yildiz
ELBÜKEN, ÇAĞLAR
DANA, Aykutlu
We demonstrate a surface plasmon resonance imaging platform integrated with a smartphone to be used in the field with high-throughput biodetection. Inexpensive and disposable SPR substrates are produced by metal coating of commercial Blu-ray discs. A compact imaging apparatus is fabricated using a 3D printer which allows taking SPR measurements from more than 20.000 individual pixels. Real-time bulk refractive index change measurements yield noise equivalent refractive index changes as low as 4.12 x 10(-5) RIU which is comparable with the detection performance of commercial instruments. As a demonstration of a biological assay, we have shown capture of mouse IgG antibodies by immobilized layer of rabbit anti-mouse (RAM) IgG antibody with nanomolar level limit of detection. Our approach in miniaturization of SPR biosensing in a cost-effective manner could enable realization of portable SPR measurement systems and kits for point-of-care applications. (C) 2016 Published by Elsevier B.V.