Assignment of biological functions to specific plasmids in a local isolate of Rhizobium cicer

Date

1999-01-01

Author

Yıldız, Eda
Özcengiz, Gülay
İçgen, Bülent
Alaeddınoglu, Ng

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The genetic basis of symbiosis, mucoid structure and melanin biosynthesis in a local isolate of Rhizobium cicer was investigated. The strain was avery effective symbiont and produced substantial amounts of exopolysaccharides and melanin. It harbored three high-molar-mass plasmids of 55, 80 and 130 kb, respectively. Thirty-one melanin production-negative (Mep(-)) derivatives were obtained through plasmid curing. The plasmid patterns of cured derivatives indicated that the loss of 55 and/or 80 kb plasmids was sufficient for obtaining Mep- phenotype. The specific involvement of 55-kb plasmid in melanin production was confirmed upon re-introduction of this plasmid into one of the Mep- derivatives. Further investigation also indicated that the 130-kb plasmid might be necessary for both mucoidal appearance and symbiotic functions.

Subject Keywords

Microbiology, General Medicine

URI

https://hdl.handle.net/11511/41876

Journal

FOLIA MICROBIOLOGICA

DOI

https://doi.org/10.1007/bf02816222

Collections

Department of Biology, Article

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Citation Formats

E. Yıldız, G. Özcengiz, B. İçgen, and N. Alaeddınoglu, “Assignment of biological functions to specific plasmids in a local isolate of Rhizobium cicer,” FOLIA MICROBIOLOGICA, pp. 55–58, 1999, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/41876.