EFFICIENCY OF GENE-TRANSFER BY CONJUGATION AND ELECTROPORATION IN LACTOCOCCI AND PEDIOCOCCI

Date

1994-08-01

Author

ALTAY, G
BOZOGLU, F
RAY, B

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

184
views

0
downloads

Sucrose-fermenting ability and nisin-producing traits were transferred to Lactococcus lactis strains by direct plate conjugation. (DPC), solid surface conjugation (SSC) and membrane filter conjugation. (MFC) with frequencies ranging from 2.6 x 10(-6) to 8.9 x 10(-10) transconjugant/donor colony forming unit (cfu). Plasmid pGK12 was transferred into plasmid-free Pediococcus acidilactici F3 by electroporation with a transfer frequency of 49 transformants/mu g DNA used.

Subject Keywords

Food Science, Microbiology, General Medicine

URI

https://hdl.handle.net/11511/66849

Journal

FOOD MICROBIOLOGY

DOI

https://doi.org/10.1006/fmic.1994.1029

Collections

Department of Food Engineering, Article

Suggestions

OpenMETU
Core

Assignment of biological functions to specific plasmids in a local isolate of Rhizobium cicer Yıldız, Eda; Özcengiz, Gülay; İçgen, Bülent; Alaeddınoglu, Ng (Springer Science and Business Media LLC, 1999-01-01) The genetic basis of symbiosis, mucoid structure and melanin biosynthesis in a local isolate of Rhizobium cicer was investigated. The strain was avery effective symbiont and produced substantial amounts of exopolysaccharides and melanin. It harbored three high-molar-mass plasmids of 55, 80 and 130 kb, respectively. Thirty-one melanin production-negative (Mep(-)) derivatives were obtained through plasmid curing. The plasmid patterns of cured derivatives indicated that the loss of 55 and/or 80 kb plasmids was...
Evaluation of high hydrostatic pressure sensitivity of Staphylococcus aureus and Escherichia coli O157 : H7 by differential scanning calorimetry Alpas, Hami; BOZOGLU, F; KALETUNC, G (Elsevier BV, 2003-11-01) Differential scanning calorimetry (DSC) was used to evaluate the relative high hydrostatic pressure (HHP) resistances of bacterial strains from Staphylococcus aureus and Escherichia coli O157:H7 in vivo. The total apparent enthalpy change and thermal stability were two DSC parameters used to compare bacterial strains of untreated control and pressure-treated bacteria. DSC thermograms indicated that ribosomal denaturation appears to be a major factor in cell death by both thermal and high pressure treatments...
Characterization of extracellular beta-lactamases from penicillin G-resistant cells of Streptococcus thermophilus Chirica, LC; Güray, Nülüfer Tülün; Gültekin, Güzin Candan; Bozoglu, F (International Association for Food Protection, 1998-07-01) In this study, biochemical properties of two extracellular beta-lactamases produced by penicillin-resistant Streptococcus thermophilus cells were investigated. Both beta-lactamases showed specificity for penicillins but not for cephaloridins. The p-lactamases exhibited different affinities for penicillin G. The one with the higher molecular weight (F1) had a K(m) value of 3.44 mu M and a V(max), value of 8.33, mu mol/min/mg of protein, whereas the beta-lactamase with the lower molecular weight (FII) had a K...
Effects of plasmid curing on antibiotic susceptibility, phage type, lipopoly saccharide and outer membrane protein profiles in local Salmonella isolates İçgen, Bülent; Gültekin, Güzin Candan; Özcengiz, Gülay (Elsevier BV, 2001-12-01) Five different Salmonella isolates (four Salmonella enteritidis and one Salmonella havana) of human, chicken or egg origin which all expressed smooth lipopolysaccharide were subjected to plasmid curing Upon this treatment, four isolates lost at least one of their resident plasmids while all five isolates became rough in their LPS profiles, Two of the S. enteritidis isolates, CA13 and CE7 were found to convert phage types (PTs) as well. The phage type conversion in CA13 from PT6 to PT21 was found to be accom...
Immunization of the industrial fermentation starter culture strain of Saccharomyces cerevisiae to a contaminating killer toxin-producing Candida tropicalis Izgu, F; Altinbay, D; Derinel, Y (Elsevier BV, 2004-12-01) K3 killer trait was introduced into the fermentation starter strain of Saccharomyces cerevisiae BSP 1 in order to construct immune industrial strain that produces K3 type killer toxin and was resistant to Candida tropicalis (K+) contamination. Protoplasts of respiration-deficient Rhodegrees strain of S. cerevisiae NCYC 761 (K3) and S. cerevisiae BSP 1 were fused. The resulting respiration-competent hybrid with K3 type killer activity was selected on media containing a non-fermentable carbon source and by a ...

Citation Formats

G. ALTAY, F. BOZOGLU, and B. RAY, “EFFICIENCY OF GENE-TRANSFER BY CONJUGATION AND ELECTROPORATION IN LACTOCOCCI AND PEDIOCOCCI,” FOOD MICROBIOLOGY, pp. 265–270, 1994, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/66849.