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EFFICIENCY OF GENE-TRANSFER BY CONJUGATION AND ELECTROPORATION IN LACTOCOCCI AND PEDIOCOCCI
Date
1994-08-01
Author
ALTAY, G
BOZOGLU, F
RAY, B
Metadata
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This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
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Sucrose-fermenting ability and nisin-producing traits were transferred to Lactococcus lactis strains by direct plate conjugation. (DPC), solid surface conjugation (SSC) and membrane filter conjugation. (MFC) with frequencies ranging from 2.6 x 10(-6) to 8.9 x 10(-10) transconjugant/donor colony forming unit (cfu). Plasmid pGK12 was transferred into plasmid-free Pediococcus acidilactici F3 by electroporation with a transfer frequency of 49 transformants/mu g DNA used.
Subject Keywords
Food Science
,
Microbiology
,
General Medicine
URI
https://hdl.handle.net/11511/66849
Journal
FOOD MICROBIOLOGY
DOI
https://doi.org/10.1006/fmic.1994.1029
Collections
Department of Food Engineering, Article