Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
PURIFICATION OF NADH-CYTOCHROME-B5 REDUCTASE FROM SHEEP LUNG AND ITS ELECTROPHORETIC, SPECTRAL AND SOME OTHER PROPERTIES
Date
1990-01-01
Author
Güray, Nülüfer Tülün
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
124
views
0
downloads
Cite This
1. 1. NADH-cytochrome b5 reductase was purified from sheep lung microsomes in the presence of non-ionic and ionic detergents. Emulgen 913 and cholate, respectively. 2. 2. The purification procedure involved the ion-exchange chromatography of the detergent solubilized microsomes on DEAE-cellulose. 3. 3. Further purification and concentration of lung reductase was carried out with a second DEAE-cellulose column followed by the affinity column chromatography of partially purified reductase on 5'-ADP-agarose column. 4. 4. The specific activity of sheep lung reductase was 638 μ mol ferricyanide reduced/min/mg protein and the yield was 6% of the initial activity in microsomes. 5. 5. The SDS-polyacrylamide gel electrophoresis of the purified lung reductase showed one protein band having the monomer mol. wt of 34,500 ± 1500. In the presence of 0.4% deoxycholate, it existed as an active dimer having a mol. wt of 68,500. 6. 6. Trypsin treated lung reductase showed two extra protein bands of mol. wts of 28,000 and 25,000 on 10% SDS-polyacrylamide gels. 7. 7. The purified enzyme was found to contain FAD as prosthetic group and the absorption spectrum of lung reductase showed two peaks at 390 and 461 nm which were typical for flavoproteins and a shoulder at 490 nm. 8. 8. The maximal activity of lung reductase was observed between pH 6.5–8.0 and at pH 6.8, when ferricyanide and partially purified sheep lung cytochrome b5, was used as electron acceptors, respectively.
Subject Keywords
Biochemistry
URI
https://hdl.handle.net/11511/42162
Journal
INTERNATIONAL JOURNAL OF BIOCHEMISTRY
DOI
https://doi.org/10.1016/0020-711x(90)90210-t
Collections
Department of Biology, Article
Suggestions
OpenMETU
Core
ELECTROPHORETIC, SPECTRAL, CATALYTIC AND IMMUNOCHEMICAL PROPERTIES OF HIGHLY PURIFIED CYTOCHROME-P-450 FROM SHEEP LUNG
Adalı, Orhan (Elsevier BV, 1990-01-01)
1. 1. Cytochrome P-450LgM2 was purified from sheep lung microsomes in the presence of detergents, Emulgen 913 and cholate. 2. 2. The purification procedure involved the chromatography of the detergent solubilized microsomes on DEAE-cellulose and hydroxylapatite. 3. 3. Cytochrome P-450LgM2 was further purified on second DEAE-cellulose and hydroxylapatite columns. 4. 4. The specific content of the highly purified P-450LgM2 was 16–18 nmol P-450/mg protein and purified 164-fold. 5. 5. The yield was 16% of t...
SOLUBILIZATION AND PARTIAL-PURIFICATION OF 2 FORMS OF CYTOCHROME-P-450 FROM TROUT LIVER-MICROSOMES
ARINC, E; Adalı, Orhan (Elsevier BV, 1983-01-01)
1. 1. Liver microsomal cytochrome P-450 content, NADPH-cytochrome reductase, aniline 4-hydroxylase and ethylmorphine activities of rainbow trout, Salmo gairdneri, were found to be 0.16 () nmol P-450/mg protein, 38 () units/mg protein, 0.04 () nmol protein and 0.174 ( ) nmol formaldehyde/min/mg protein, respectively. 2. 2. Trout liver cytochrome P-450 was solubilized by treatment of microsomes with sodium cholate. Chromatography on DEAE-cellulose column yielded two distinct cytochrome P-450 f...
Synthesis of diols using the hypervalent iodine(III) reagent, phenyliodine(III) bis(trifluoroacetate)
Celik, Murat; Alp, Cemalettin; Coşkun, Betül; Gultekin, M. Serdar; Balcı, Metin (2006-05-29)
1.2- and 1.3-Bis(trifluoroacetoxy) alcohols are easily obtained from the one-pot reaction of alkenes with phenyliodine(III) bis(trifluoroacetate) (PIFA) in the absence of any additive or catalyst. The products were converted into the corresponding diols by ammonolysis. The use of bicyclic alkenes has shown that rearranged 1,3-diacetoxy alcohols are mostly formed as the major products.
SYNTHESIS AND STEREOCHEMISTRY OF PENTACARBONYL(PHOSPHOLE)METAL(0) AND TETRACARBONYLBIS(PHOSPHOLE)METAL(0) COMPLEXES OF 6B ELEMENTS
OZER, Z; Özkar, Saim (Informa UK Limited, 1992-01-01)
Pentacarbonylphospholemetal(0) and cis-tetracarbonylbis(phosphole)metal(0) complexes were synthesized from the thermal reaction of M(CO)5(THF) and M(CO)4(COD) (M: Cr, Mo, W) with corresponding phosphole (1-phenyl-3,4-dimethylphosphole, 1-phenyl-3-methylphosphole, and 1-phenylphosphole). These complexes were isolated as orange crystals by column chromatography on silicagel at 253 K and crystallization from n-hexane at 223 K and characterized by means of IR and NMR (H-1, C-13, and P-31). Spectroscopic data sh...
Selective recognition of anionic cell membranes using targeted liposomes coated with zinc(II)-bis(dipicolylamine) affinity units
Türkyılmaz, Serhan; Palumbo, Rachael; Smith, Bradley D. (Royal Society of Chemistry (RSC), 2014-08-14)
Zinc(II)-bis(dipicolylamine) (Zn(2)BDPA) coated liposomes are shown to have high recognition selectivity towards vesicle and cell membranes with anionic surfaces. Robust synthetic methods were developed to produce Zn(2)BDPA-PEG-lipid conjugates with varying PEG linker chain length. One conjugate (Zn(2)BDPA-PEG(2000)-DSPE) was used in liposome formulations doped with the Lipophilic near-infrared fluorophore DiR. Fluorescence cell microscopy studies demonstrated that the multivalent Liposomes selectively and ...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
N. T. Güray, “PURIFICATION OF NADH-CYTOCHROME-B5 REDUCTASE FROM SHEEP LUNG AND ITS ELECTROPHORETIC, SPECTRAL AND SOME OTHER PROPERTIES,”
INTERNATIONAL JOURNAL OF BIOCHEMISTRY
, pp. 1029–1029, 1990, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/42162.