Neuropeptide Y is involved in the regulation of quiescence of hematopoietic stem cells

Date

2020-04-01

Author

Ulum, Baris
Mammadova, Aynura
ÖZYÜNCÜ, ÖZGÜR
Uckan-Cetinkaya, Duygu
Yanık, Tülin
Aerts-Kaya, Fatima

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

197
views

0
downloads

Differentiation, self-renewal and quiescence of Hematopoietic stem cells (HSCs) is tightly regulated in order to protect the HSCs from the strain of constant cell division and depletion of the stem cell pool. The neurotransmitter Neuropeptide Y (NPY) is released from sympathetic nerves in the bone marrow and has been shown to indirectly affect HSC function through effects on bone marrow (BM) multipotent Mesenchymal Stromal Cells (MSCs), osteoblasts (OBs) and macrophages. Although the absence of NPY has been shown to be accompanied by severe BM impairment and delayed engraftment of HSCs, the direct effects of NPY on HSCs have never been assessed. Here, we aimed to explore the effect of NPY on the regulation of HSCs. All NPY receptors Y1, Y2, Y4 and Y5 were found to be highly expressed on most HSCs and mature hematopoietic cell subsets. In culture, in particularly expression of the Y1 receptor was shown to decrease in time. Doses of 300 nM NPY suppressed HSC proliferation in cell cultures, as confirmed by an increase of HSCs in G(0) phase and an increase in the gene expression levels of FOXO3, DICERI, SMARCA2 and PDK1, which all have been shown to play an important role in the regulation of cell quiescence. These data support the idea that NPY may have a direct effect on the regulation of HSC fate by modulating cell quiescence.

Subject Keywords

Endocrinology, Cellular and Molecular Neuroscience, Endocrine and Autonomic Systems, Neurology, General Medicine

URI

https://hdl.handle.net/11511/42909

Journal

NEUROPEPTIDES

DOI

https://doi.org/10.1016/j.npep.2020.102029

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Poly( amino acid)-based fibrous scaffolds modified with surface-pendant peptides for cartilage tissue engineering Svobodova, Jana; Proks, Vladimir; Karabiyik, Ozge; Koyuncu, Ayse Ceren Calikoglu; Kose, Gamze Torun; Rypacek, Frantisek; Studenovska, Hana (Wiley, 2017-03-01) In this study, fibrous scaffolds based on poly(gamma-benzyl-L-glutamate) (PBLG) were investigated in terms of the chondrogenic differentiation potential of human tooth germ stem cells (HTGSCs). Through the solution-assisted bonding of the fibres, fully connected scaffolds with pore sizes in the range 20-400 mu m were prepared. Biomimetic modification of the PBLG scaffolds was achieved by a two-step reaction procedure: first, aminolysis of the PBLG fibres' surface layers was performed, which resulted in an i...
Epigenetic Mechanisms Underlying the Dynamic Expression of Cancer-Testis Genes, PAGE2, -2B and SPANX-B, during Mesenchymal-to-Epithelial Transition Yilmaz-Ozcan, Sinem; Sade, Asli; Kucukkaraduman, Baris; Kaygusuz, Yasemin; Senses, Kerem Mert; Banerjee, Sreeparna; GÜRE, ALİ OSMAY (Public Library of Science (PLoS), 2014-09-17) Cancer-testis (CT) genes are expressed in various cancers but not in normal tissues other than in cells of the germline. Although DNA demethylation of promoter-proximal CpGs of CT genes is linked to their expression in cancer, the mechanisms leading to demethylation are unknown. To elucidate such mechanisms we chose to study the Caco-2 colorectal cancer cell line during the course of its spontaneous differentiation in vitro, as we found CT genes, in particular PAGE2, -2B and SPANX-B, to be up-regulated duri...
Rapid differentiation of MSC into adipocytes and osteocytes is facilitated by Toll-Like receptor engagement Tas, Ibrahim; Tokcaer-Keskin, Zeynep; Ayhan, Fatma; Gürsel, Mayda; Akcali, Kamil (2010-04-01) Mesenchymal stem cells are multipotent progenitor cells capable of differentiating into several lineages including adipocytes, and osteocytes. While they are regarded as non-immunogenic, accumulating evidence suggests that MSC expresses several TLRs. We checked the contribution of TLR ligands to MSC differentiation. Rat bone marrow derived MSC surface marker and TLR transcript levels were checked by FACS and RT-PCR respectively. MSCs were CD29hi, CD71dim, CD90hi, and negative for CD45 & CD34. They expressed...
CpG ODN Nanorings Induce IFN alpha from Plasmacytoid Dendritic Cells and Demonstrate Potent Vaccine Adjuvant Activity Gungor, Bilgi; Yagci, Fuat Cem; Tincer, Gizem; Bayyurt, Banu; Alpdundar, Esin; Yildiz, Soner; Ozcan, Mine; GÜRSEL, İHSAN; Gürsel, Mayda (American Association for the Advancement of Science (AAAS), 2014-05-07) CpG oligodeoxynucleotides (ODN) are short single-stranded synthetic DNA molecules that activate the immune system and have been found to be effective for preventing and treating infectious diseases, allergies, and cancers. Structurally distinct classes of synthetic ODN expressing CpG motifs differentially activate human immune cells. K-type ODN (K-ODN), which have progressed into human clinical trials as vaccine adjuvants and immunotherapeutic agents, are strong activators of B cells and trigger plasmacytoi...
NEUROSECRETORY-CELLS WITH SYNAPTOID PERIKARYA IN HELIX - A DEFINITIVE DESCRIPTION OF SECRETORY RELEASE FROM THE SOMATA OF ENDOCRINE NEURONS BAYRAKTAROGLU, E; WHITTLE, AC; GOLDING, DW (Elsevier BV, 1988-01-01) Neurosecretory cells in the molluses Helix have perikarya that show clear signs of adaptation for both the synthesis and the discharge of secretory material. They are characterized morphologically by juxtaposition with the neural lamella. Passage of hormone into the haemocoel is apparently facilitated in many cases by the extreme attenuation of areas of the lamella and perineurium adjacent to the perikarya and by other forms of histological differentiation. Presumptive sites of release are characterized by ...

Citation Formats

B. Ulum, A. Mammadova, Ö. ÖZYÜNCÜ, D. Uckan-Cetinkaya, T. Yanık, and F. Aerts-Kaya, “Neuropeptide Y is involved in the regulation of quiescence of hematopoietic stem cells,” NEUROPEPTIDES, pp. 0–0, 2020, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/42909.