Protoplast isolation and culture in sugar beet (Beta vulgaris L.)

Gürel, Ekrem
Gürel, Songül
Kaya, Zeki
In this study, attempts were made to optimise conditions for protoplast isolation from suspension and mesophyll cells, growth of isolated protoplasts and formation of callus from the cultured protoplasts. The enzyme mixture containing 1.0% cellulase, 0.1% hemicellulase, 1.0% pectinase and 0.1% driselase was found to be the most suitable combination for yield and viability of both suspension-and mesophyll-derived protoplasts from sugar beet. Genotypic variation for plating efficiency was significant; ELK345and M1017 lines producing significantly higher plating efficiency (PE) than other lines. On the other hand, suspension-derived protoplasts of line ELK345 formed more colonies than mesophyll-derived protoplasts of the same line. Micro-callus was obtained from only suspension-derived protoplasts of line ÇBM315. The effects of densities on PE of protoplast cultures were significant -i.e., the highest initial plating density (3 × 105 protoplasts ml-1) produced the highest PE. The differences between the levels of BAP or 2,4-D on colony formation of protoplasts were not significant. However, media containing combinations of BAP or 2,4-D were more efficient for cell colony formation than the control (i.e., hormone-free) medium.
Plant Cell Biotechnology and Molecular Biology


Intracellular alkaline proteases produced by thermoacidophiles: detection of protease heterogeneity by gelatin zymography and polymerase chain reaction (PCR)
Kocabıyık, Semra (2002-08-01)
In this study 24 thermoacidophilic archeal and bacterial strains isolated from hot-springs and hot-soils were screened for their ability to produce intracellular alkaline proteases. The protease activities of the strains, based on azocasein hydrolysis, showed a variation from 0.6 to 5.1 U. The cell extracts of three most potent producers were further examined and it was found that their proteases exhibited maximum activity at 60-70 degreesC and showed a pH Optimum over a range of pH 7.0-8.5. Gelatin zymogra...
Synthesis of 2-aziridinyl phosphonates by modified Gabriel-Cromwell reaction and their antibacterial activities
Doğan, Özdemir; Gözen, Ayşe Gül (Elsevier BV, 2011-06-01)
A set of new aziridinyl phosphonates (4a-g) were synthesized by using the Gabriel-Cromwell reaction and its modified version developed in this study and their structures confirmed by HRMS, IR. and NMR spectra. All the compounds were screened for their antibacterial activity. They all showed comparable moderate to good growth inhibitory activity in reference to ampicillin and streptomycin. (C) 2011 Elsevier Masson SAS. All rights reserved.
Transcriptome analysis of thermoplasma volcanium GSS1 under extreme stress
Zabcı, Sema; Kocabıyık, Semra; Department of Biology (2015)
In this study, to provide new insight into anti-stress mechanisms in archaea, we have carried out genome wide transcriptome analysis of Thermoplasma volcanium under severe stress conditions (i.e., heat- shock at 68 ºC, sub-lethal and lethal oxidative stress and pH stress at pH 5.0) using whole genome gene expression microarray. The change in the gene expression profile in response to three stressors indicated retardation of cell growth accompanied by down regulation of genes involved in essential cellular p...
Alterations of ceramide/sphingosine 1-phosphate rheostat involved in the regulation of resistance to imatinib-induced apoptosis in K562 human chronic myeloid leukemia cells
Baran, Yusuf; Salas, Arelis; Senkal, Can E.; Gündüz, Ufuk; Bielawski, Jacek; Obeid, Lina M.; Ogretmen, Besim (2007-04-13)
In this study, mechanisms of resistance to imatinib-induced apoptosis in human K562 cells were examined. Continuous exposure to stepwise increasing concentrations of imatinib resulted in the selection of K562/IMA-0.2 and -1 cells, which expressed similar to 2.3- and 19-fold resistance, respectively. Measurement of endogenous ceramides by high performance liquid chromatography/mass spectroscopy showed that treatment with imatinib increased the generation of ceramide, mainly C-18-ceramide, which is generated ...
Optimization of regeneration and agrobacterium mediated transformation of sugar beet (Beta vulgaris L.)
Baloğlu, Mehmet Cengiz; Yücel, Ayşe Meral; Department of Biology (2005)
In this study, optimization of a transformation and regeneration system via indirect and direct organogenesis in cotyledon, hypocotyl, petiole, leaf and shoot base tissues of sugar beet (Beta vulgaris L. cv. ELK 345 and 1195) was investigated. Two different germination, three different callus induction and shoot induction medium was used for indirect organogenesis of sugar beet cultivar ELK 345. Except cotyledon, other explants (hypocotyl, petiole and leaf) produced callus. However no shoot development was ...
Citation Formats
E. Gürel, S. Gürel, and Z. Kaya, “Protoplast isolation and culture in sugar beet (Beta vulgaris L.),” Plant Cell Biotechnology and Molecular Biology, pp. 11–20, 2002, Accessed: 00, 2021. [Online]. Available: