Historical development of simulated body fluids used in biomedical applications: A review

Simulated body fluid (SBF) is an acellular, protein-free, supersaturated calcium-phosphate solution with ionic composition nearly equal to that of human blood plasma and generally buffered at physiological conditions (pH 7.4 and 36.5 degrees C). Various SBF formulations have been used for decades to: (1) test corrosion and bone-bonding behavior, bioactivity, ion release, swelling and biodegradation rates of biomaterials and (2) deposit calcium phosphates, especially hydroxyapatite, onto the substrates of different kinds by biomimetic and electrochemical methods. This review reports the wide range of compositional variations in SBF solutions and reviews the studies in biomedical area where SBF has been used for a number of different purposes, such as increasing the osseointegration (direct bonding with bone) capability of implant biomaterials or testing the corrosion rate of metallic biomaterials.


ERGUCYENER, C; AYGUN, S; Ataman, Osman Yavuz; TEMIZER, A (1988-01-01)
Mercury speciation in aqueous solutions containing inorganic mercury and methylmercury has been demonstrated using electrochemical separation prior to determination by cold-vapour atomic absorption spectrometry. Approximately 95% of the inorganic mercury was electro-deposited on a Pt electrode at –0.1 V, whereas for methylmercury –0.2 V or even more negative potentials were required. Organic mercury was determined by NaBH4 reduction after electrochemical separation. Inorganic mercury was determined in the o...
Quality improvement in determination of chemical oxygen demand in samples considered difficult to analyze, through participation in proficiency-testing schemes
Raposo, F.; Fernandez-Cegri, V.; De la Rubia, M. A.; Borja, R.; Beltran, J.; Cavinato, C.; Clinckspoor, M.; Demirer, Göksel Niyazi; Diamadopoulos, E.; Frigon, J. C.; Koubova, J.; Launay, M.; Mendez, R.; Menin, G.; Noguerol, J.; Uellehdahl, H.; West, S. (Elsevier BV, 2010-10-01)
Chemical oxygen demand (COD) is a critical analytical parameter in waste and wastewater treatment, more specifically in anaerobic digestion, although little is known about the quality of measuring COD of anaerobic digestion samples. Proficiency testing (PT) is a powerful tool that can be used to test the performance achievable in the participants' laboratories, so we carried out a second PT of COD determination in samples considered "difficult" to analyze (i.e. solid samples and liquid samples with high con...
Optimization of ETV-ICP(TOF)MS and transient signal profiles for reducing isobaric interferences
Ertaş, Gülay (Royal Society of Chemistry (RSC), 2005-01-01)
One of the advantages of the ETV sample introduction is the ability to temporally separate analyte elements in complex mixtures by differences in their vaporization temperatures within the ETV for ICPMS. However, the broadening of the transient peaks in the transport tubing often obscures this temporal resolution. This study shows that decreasing the transport tubing diameter produces little broadening beyond that produced during aerosol production in the ETV. Maintaining such narrow peaks through the trans...
Raman Spectroscopy Investigation of Nano Hydroxyapatite Doped with Yttrium and Fluoride Ions
Yilmaz, Bengi; Evis, Zafer (Informa UK Limited, 2014-01-02)
In this study, nano hydroxyapatite doped with yttrium (2.5, 5, and 7.5mol%) and fluoride (2.5mol%) ions were synthesized by precipitation method and sintered at 900 degrees C, 1100 degrees C, and 1300 degrees C. Raman spectroscopy was applied to track the structural modifications in pure and doped hydroxyapatites. The results showed that the main characteristic band of pure hydroxyapatite at 963cm(-1) was not affected significantly by ion doping but exhibited higher intensity with increasing sintering tempe...
Introduction of solid-phase microextraction as a high-throughput sample preparation tool in laboratory analysis of prohibited substances
Boyacı, Ezel; Rodriguez-Lafuente, Angel; Bojko, Barbara; Pawliszyn, Janusz (Elsevier BV, 2014-01-27)
A fully automated, high-throughput method based on thin-film solid-phase microextraction (SPME) and liquid chromatography-mass spectrometry was developed for simultaneous quantitative analysis of 110 doping compounds, selected from ten classes and varying in physical and chemical properties. Among four tested extraction phases, C18 blades were chosen, as they provided optimum recoveries and the lowest carryover effect. The SPME method was optimized in terms of extraction pH, ionic strength of the sample, wa...
Citation Formats
B. Yilmaz, A. E. Pazarçeviren, A. Tezcaner, and Z. Evis, “Historical development of simulated body fluids used in biomedical applications: A review,” MICROCHEMICAL JOURNAL, pp. 0–0, 2020, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/43337.