Genetic characterization of pear cultivars (Pyrus communis) in Çoruh river basin

Download

index.pdf

Date

2019

Author

Çoban, Abdulbaki

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

282
views

174
downloads

The genus Pyrus contains more than 20 different pear species which are used as food source, horticultural, and ornamental purposes. Overall, pear is the second most consumed pome fruit after apple. P. communis is the most cultivated pear species in Europe and Asia Minor. However, more than 10 species of genus Pyrus can be found naturally in Europe and Asia Minor. In this study, 8 P. communis populations (one wild and 7 cultivated) and 1 P. eleagnifolia population was used to reveal genetic structure of those populations by using 11 SSR markers previously used in genus Pyrus and Malus. 84 genotypes of P. communis were sampled from Artvin Province, Turkey and 20 genotypes of P. eleagnifolia were sampled from METU campus in Ankara, Turkey for the current study. Overall, there were no null alleles in the studied populations. There is no duplicated genotypes and no linkage between loci were found. Expected heterozygosity, allelic richness, and polymorphic information content were calculated to check the usefulness of the studied loci. All loci were found to be highly polymorphic for the further studies. Structure analysis of the studied populations of P. communis revealed that there are significant gene flow between populations. Therefore, no clear population vi differentiation was found. AMOVA results supported this finding as most of the differentiation was among genotypes within population. However, the wild population of P. communis were found to be distinct from other populations. Thus, it is summed as during domestication of P. communis, there were hybridization between genotypes within and between populations. As for the genetic structure analysis of P. communis and P. eleagnifolia populations, it was revealed that P. communis and P. eleagnifolia populations were significantly different from each other as expected. Since those are totally different species and due to geographical isolation, there were no gene flow between those populations. The study was one of the first studies conducted on P. communis populations in Turkey. Thus, outcomes of the study are important for possible further studies which will be conducted on genus Pyrus. Besides, findings are important for further conservation studies of P. communis genotypes and breeding studies.

Subject Keywords

Common pear., Pyrus communis, Pyrus eleagnifolia, SSR, population structure, Genetic diversity.

URI

http://etd.lib.metu.edu.tr/upload/12623189/index.pdf
https://hdl.handle.net/11511/43359

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Genetic differentiation between clone collections and natural populations of European black poplar (Populus nigra L.) in turkey Çiftçi, Asiye; Kucukosmanoglu, Filiz; Karahan, Alptekin; Kaya, Zeki (2017-06-01) The European black poplar (Populus nigra L.) is an ecologically and economically important tree species for Turkey. The important and major genetic resources of species for future breeding and ex situ conservation purposes have been archived in a clone bank in Ankara by selecting clones from natural populations and old plantations throughout Turkey. There is no study to date assessing genetic composition these materials. Two-hundred-thirty-three P. nigra clones from six geographic region of Turkey (clone co...
Genetic relationships among perennial and annual Cicer species growing in Turkey as revealed by allozymes Sudupak, MA; Kence, Aykut (Springer Science and Business Media LLC, 2004-05-01) Allozyme polymorphisms were used to assess genetic variation and relationships among ten Cicer species ( annuals and perennials) growing in Turkey. Using seven enzyme systems, 12 putative scorable loci were detected and surveyed for polymorphism in an accession collection including wild and cultivated forms. Variation was generally low within accessions and species, but common between species. Cluster analysis based on the pairwise genetic distance coefficients (Nei 1978) among accessions and species using ...
Phylogeography and phylogeny of genus Quercus L. (Fagaceae) in Turkey implied by variations of trnT (UGU) -L (UAA) -F (GAA) chloroplast DNA region Tekpinar, Ayten Dizkirici; AKTAŞ, CANER; Kansu, Çiğdem; DUMAN, HAYRİ; Kaya, Zeki (2021-10-01) The genus Quercus L. is one of the most abundant and important genera of woody plants in the Northern Hemisphere as well as in Turkey. In the current study which is the most comprehensive study dealing with Turkish oaks, sequence variations of three noncoding regions (trnT(UGU)-L(UAA) IGS, trnL(UAA)intron, trnL(UAA)-F(GAA) IGS) of chloroplast DNA (cpDNA) were used for phylogeographic and phylogenetic analysis on 319 individuals representing 23 taxa (17 species). The trnT(UGU)-L(UAA) region was found to be t...
Genetic structure of black pine (Pinus nigra ARNOLD subspecies pallasiana) populations sampled from the Bolkar Mountains Tolun, AA; Velioğlu, E; Çengel, Burcu N; Kaya, Zeki (2000-01-01) In order to determine the magnitude and pattern of genetic diversity among Anatolian Black pine (Pinus nigra ARNOLD subspecies pallasiana) populations sampled in Bolkar Mountains and to recommend the potential populations which may be suitable for in situ conservation of genetic resources in this species, isoenzymes from 14 enzyme systems were investigated by starch gel electrophoresis. For this reason, open pollinated seed megagametophytes of half-sib families originated from the four populations (Camliyay...
Taxonomic revision of six Astragalus sections that native to Turkey based on non-coding trn regions of cpDNA Ateş, Mevlüde Alev; Karaman Erkul, Seher; Önde, Sertaç; Aytaç, Zeki; Kaya, Zeki (null; 2018-01-08) Astragalus, belonging to the legume family Fabaceae, is a large genus with about 3,000 species of herbs and small shrubs in Northern Hemisphere.The aim of this study is to revise Macrophyllium, Hymenostegis, Poterion, Megalocystis, Halicacabus and Hymenocoleus sections of genus Astragalus naturally found in Turkey by using three noncoding trn regions from chroloplast DNA.

Citation Formats

A. Çoban, “Genetic characterization of pear cultivars (Pyrus communis) in Çoruh river basin,” Thesis (M.S.) -- Graduate School of Natural and Applied Sciences. Molecular Biology and Genetics, Middle East Technical University, 2019.