Genetic differentiation between clone collections and natural populations of European black poplar (Populus nigra L.) in turkey

Çiftçi, Asiye
Kucukosmanoglu, Filiz
Karahan, Alptekin
Kaya, Zeki
The European black poplar (Populus nigra L.) is an ecologically and economically important tree species for Turkey. The important and major genetic resources of species for future breeding and ex situ conservation purposes have been archived in a clone bank in Ankara by selecting clones from natural populations and old plantations throughout Turkey. There is no study to date assessing genetic composition these materials. Two-hundred-thirty-three P. nigra clones from six geographic region of Turkey (clone collection populations), and 32 trees from two natural populations (Tunceli and Melet) were genotyped by using 12 nuclear microsatellite DNA markers. There were nine clones which duplicated in various frequencies. The analysis carried out with removal of the duplicated clones revealed a moderately high genetic diversity in studied populations. The observed heterozygosities ranged from 0.59 in Tunceli natural to 0.69 in Central Anatolia clone collection populations. In general, there was excess of heterozygosity in the studied populations. Populations composed of clone collections were significantly differentiated from natural populations (F-ST = 0.17), while there was little differentiation among those populations in the clone collection (F-ST = 0.03). Two distantly located natural populations with small sizes also differed from each other (F-ST = 0.17). Genetic structure analysis revealed two distinct groups (clone collection vs natural populations) with very high membership values (>92%). Clone collection populations had high level of admixture while natural populations had homogenous genetic structure. The presence of large number of clonal duplication, reduced genetic differentiation, and high level of admixture in clone collection populations indicate that genetic resources of European black poplar were highly degraded through genetic erosion and pollution caused by intensive cultural practices and extensive dispersal of clonal materials. To understand genetic diversity and its structural pattern thoroughly in the six clone collection populations, a further study with extensive and systematic sampling of European black poplar populations in major river ecosystems in Turkey will be useful.


Genetic diversity pattern in Populus Nigra populations of two major river systems in Turkey
Çiftçi, Asiy; Kaya, Zeki; Department of Biology (2019)
European black poplar (Populus nigra L.) is an important tree species in terms of social, economic and ecological interest in Turkey. Although, poplar plantations in large areas meet the needs of the economy, the natural genetic resources of the species have been highly degraded due to anthropogenic effects and natural biological events. To assess genetic diversity structure of natural populations, 124 naturally distributed European black poplar trees from two major rivers (Kızılırmak and Göksu) in Turkey w...
Genetic differentiation of European black poplar (Populus Nigra L.) clones and populations with respect to some enzymes involved in biosynthesis of cellulose and lignin
Taşkıran, Bircan; Kaya, Zeki; Department of Biology (2020)
The European black poplar, (Populus nigra L.) is ecologically and economically important fast-growing trees. Its wide distribution range in northern hemisphere, easy vegetative propagation, rapid growth rate and hybridization ability, and also its biomass source potential make European black poplar a perfect candidate for lignocellulosic biomass production. In this study, specific activities of two important cellulose related (SuSy and UGPase) and three lignin pathway related (PAL, 4CL, and CAD) enzymes, we...
Genetic variation in Turkish red pine (Pinus brutia Ten.) seed stands as determined by RAPD markers
Kandedmir, GE; Kandemir, I; Kaya, Zeki (2004-01-01)
Turkish red pine (Pinus brutia Ten.) is one of the important tree species in Turkey. The species has been heavily disturbed by anthropogenic factors and fire, making it important to understand the magnitude of the impact on genetic diversity of areas reserved for seed production. To explore the extent of genetic variation in Turkish red pine seed stands, a random amplified polymorphic DNA (RAPD) marker system was used in the study. The estimated genic diversity for seed stands was found to range from H=0.17...
Durgut, Bilge; Kaya, Zeki; Department of Molecular Biology and Genetics (2022-2-23)
Salix alba and Populus nigra are two important tree species of riparian ecosystems. With a great ecologic and economic significance, they are naturally distributed in almost all river basins of Turkey. The genetic structures of these species in two river basins in Turkey were studied using eight common microsatellite markers to both species to reveal the impacts of habitat fragmentation. Therefore, the studied rivers were selected to represent a highly fragmented river, and a protected river from habitat fr...
Genetic diversity and population structure of Salix alba across river systems in Turkey and their importance in conservation management
Özdemir Değirmenci, Funda; Çiftçi, Asiye; Acar, Pelin; Kaya, Zeki (2022-03-01)
Background Salix alba is a pioneer species of river ecosystems throughout Turkey. Its genetic diversity and population structure across these ecosystems is currently unknown. Aims We investigated genetic diversity in Turkish S. alba to assess factors likely to shape the genetic structure of the species and to assist with conservation recommendations. Methods Six hundred and forty-six individuals from 10 major river systems in Turkey were genotyped using 15 microsatellite markers. Between one and five sub-po...
Citation Formats
A. Çiftçi, F. Kucukosmanoglu, A. Karahan, and Z. Kaya, “Genetic differentiation between clone collections and natural populations of European black poplar (Populus nigra L.) in turkey,” TREE GENETICS & GENOMES, pp. 0–0, 2017, Accessed: 00, 2020. [Online]. Available: