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Effect of side chain variation on surface and biological properties of poly(2-alkyl-2-oxazoline) multilayers

Multilayers of poly(2-alkyl-2-oxazoline)s (PAOX) with varying side chains were constructed through layer-by-layer (LbL) self-assembly using Tannic Acid (TA). Surface morphology, stability, wettability and stimuli-responsive drug release properties of the films were correlated with antiadhesive and antibacterial properties of the multilayers. Thickness, roughness, wettability and stability of multilayers were affected by the chemical nature of PAOX side chain, phase behaviour of PAOX and the binding strength between the layers. PAOX-multilayers released remarkably different amounts of Ciprofloxacin (CIP) especially at acidic pH and 37 °C. Antibacterial activity of the films against S. aureus was in good agreement with the amounts of CIP released from multilayers. Despite the differences in the released CIP amount, PAOX-multilayers showed similar antibacterial activity against E. coli under all conditions. Multilayers with greater wettability exhibited greater resistance against Bovine Serum Albumin (BSA) adsorption and were more effective to reduce adherence of E. coli. None of the PAOX-films exhibited antiadhesiveness against S. aureus.