Evaluation of Cholinesterase Inhibitory and Antioxidant Activities of Wild and Cultivated Samples of Sage (Salvia fruticosa) by Activity-Guided Fractionation

Erdem, Sinem Aslan
Celep, Ferhat
Kahraman, Ahmet
Doğan, Musa
In European folk medicine, Salvia species have traditionally been used to enhance memory. In our previous study of 55 Salvia taxa, we explored significant anticholinesterase activity of cultivated S. fruticosa. In this study, we compared the inhibitory activity of dichloromethane, ethyl acetate, and ethanol extracts of 3 wild-grown samples and 1 cultivated sample of S. fruticosa against acetylcholinesterase and butyrylcholinesterase enzymes (which are associated with pathogenesis of Alzheimer's disease) by using the spectrophotometric Ellman method. Antioxidant activities were assessed by determining 2,2-diphenyl-1-picrylhydrazyl radical-scavenging activity, iron-chelating capacity, and ferric-reducing antioxidant power. The dichloromethane extract of the cultivated sample was then subjected to fractionation by using open column chromatography and medium-pressure liquid chromatography to obtain the most active fraction by activity-guided fractionation. All fractions and subfractions were tested in the same manner, and inactive subfractions were discarded. The essential oil of the cultivated sample was analyzed by gas chromatography-mass spectrometry.


Antioxidant and Antimicrobial Activities of Essential Oils Obtained from Oregano (Origanum vulgare ssp hirtum) by Using Different Extraction Methods
KARAKAYA, SİBEL; EL, SEDEF NEHİR; Karagozlu, Nural; Şahin, Serpil (Mary Ann Liebert Inc, 2011-06-01)
In this study, antioxidant and antimicrobial activities of essential oils obtained from oregano (Origanum vulgare ssp. hirtum) were determined by using solvent-free microwave extraction (SFME), supercritical fluid extraction, and conventional hydrodistillation (CH) methods. The inhibitory effects on the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical of essential oils obtained from oregano by using SFME and CH were similar. However, essential oil extracted by CH showed greater (2.69 mu mol/mL...
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Enayat, Shabnam; Ceyhan, Muserref Seyma; BAŞARAN, ARİF AHMET; Gürsel, Mayda; Banerjee, Sreeparna (Informa UK Limited, 2013-10-01)
The bark from Salix species of plants has been traditionally consumed for its antiinflammatory properties. Because inflammation frequently accompanies the progress of colorectal cancer (CRC), we have evaluated the anticancer properties of the ethanolic extract from the bark (EEB) of S. aegyptiaca, a Salix species endogenous to the Middle East, using HCT-116 and HT29 CRC cell lines. Fresh bark from S. aegyptiaca was extracted with ethanol, fractionated by solvent-solvent partitioning and the fractions were a...
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Elibol-Can, Birsen; Simsek-Ozek, Nihal; Severcan, Feride; Severcan, Mete; Jakubowska-Dogru, Ewa (Cambridge University Press (CUP), 2015-01-14)
To date, no structural study has been carried out on the effects of vitamin A deficiency (VAD) on hippocampal macromolecules. Therefore, in the present study, the effect of dietary VAD on the structure, content and function of rat hippocampal molecules was investigated using Fourier transform IF spectroscopy. Male Wistar rats were randomly divided into three groups: an experimental group maintained on a vitamin A-deficient liquid diet (VAD, n 7); a control group maintained on a vitamin A-supplemented liquid...
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Medicinal plants which have been widely used in folk medicine are known to contain important biologically active compounds. Hypericum perforatum is one of the medicinal plants that grows in Europe, Western Asia and Northern Africa and is distinguished by its golden yellow flowers. The common name of the plant is St. John’s wort. As a practical folk-remedy, it has been used widely to heal wounds, remedy kidney troubles, and alleviate nervous disorders. Nowadays, many application areas of hypericin are discov...
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Aim of this study was to determine the extent of pollution in the West Black Sea Coast of Turkey by measuring CYP1A associated EROD activity, phase II enzyme, glutathione S-transferase and antioxidant enzymes, catalase and glutathione reductase activities and immunochemical detection of CYP1A protein level in the liver of mullet. The fish samples were caught from six locations having a varying degree of pollution in the West Black Sea Region of Turkey in August 2005, 2006 and 2007. Mullets caught from Zongu...
Citation Formats
F. S. ŞENOL et al., “Evaluation of Cholinesterase Inhibitory and Antioxidant Activities of Wild and Cultivated Samples of Sage (Salvia fruticosa) by Activity-Guided Fractionation,” JOURNAL OF MEDICINAL FOOD, pp. 1476–1483, 2011, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/43483.