Anticarcinogenic Effects of the Ethanolic Extract of Salix aegyptiaca in Colon Cancer Cells: Involvement of Akt/PKB and MAPK Pathways

Enayat, Shabnam
Ceyhan, Muserref Seyma
Gürsel, Mayda
Banerjee, Sreeparna
The bark from Salix species of plants has been traditionally consumed for its antiinflammatory properties. Because inflammation frequently accompanies the progress of colorectal cancer (CRC), we have evaluated the anticancer properties of the ethanolic extract from the bark (EEB) of S. aegyptiaca, a Salix species endogenous to the Middle East, using HCT-116 and HT29 CRC cell lines. Fresh bark from S. aegyptiaca was extracted with ethanol, fractionated by solvent-solvent partitioning and the fractions were analyzed by tandem mass spectrometry. Catechin, catechol, and salicin were the most abundant constituents of the extract. Interestingly, EEB showed the highest anticancer effect in the colon cancer cells followed by its fractions in ethyl acetate and water, with catechin, catechol, and salicin showing the least efficacy. EEB could strongly reduce the proliferation of the cancer cells, but not of CCD-18Co, normal colon fibroblast cell line. Accompanying this was cell cycle arrest at G1/S independent of DNA damage in the cancer cells, induction of apoptosis through a p53 dependent pathway and an inhibition of PI3K/Akt and MAP Kinase pathways at levels comparable to known commercial inhibitors. We propose that the combination of the polyphenols and flavonoids in EEB contributes toward its potent anticarcinogenic effects. [Supplementary materials are available for this article. Go to the publisher's online edition of Nutrition and Cancer for the following free supplemental resource(s): Supplementary Figure 1 and Supplementary Figure 2.]


Antioxidant and Antimicrobial Activities of Essential Oils Obtained from Oregano (Origanum vulgare ssp hirtum) by Using Different Extraction Methods
KARAKAYA, SİBEL; EL, SEDEF NEHİR; Karagozlu, Nural; Şahin, Serpil (Mary Ann Liebert Inc, 2011-06-01)
In this study, antioxidant and antimicrobial activities of essential oils obtained from oregano (Origanum vulgare ssp. hirtum) were determined by using solvent-free microwave extraction (SFME), supercritical fluid extraction, and conventional hydrodistillation (CH) methods. The inhibitory effects on the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical of essential oils obtained from oregano by using SFME and CH were similar. However, essential oil extracted by CH showed greater (2.69 mu mol/mL...
Antioxidant properties of Salvia absconditiflora extracts and their effects on phase I and phase II gene expressions in HEPG2 cell line
İrtem Kartal, Deniz; Güray, Tülin; Sadi, Gökhan; Department of Biochemistry (2015)
S.absconditiflora is one of the endemic Salvia species grown in Turkey, which is consumed as a herbal tea. Because of the presence of high amounts of vesicles on their leaves, it is very rich in bioactive compounds. S.absconditiflora water and methanol extracts were studied for their antioxidant capacity by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and ABTS free radical scavenging assay. Total phenolic and total flavonoid contents were quantified by spectrophotometric methods, HPLC and LC-MS/MS analyses and the ...
Antioxidant capacities of Gundelia tournefortii L. extracts and inhibition on glutathione-S-transferase activity
Çoruh, Nursen; Ozgokce, F.; Iscan, M. (Elsevier BV, 2007-01-01)
Gundelia tournefortii L. is an important food source and a well-known medicinal plant in Eastern Anatolia. Therapeutic effects of medicinal plants are known to be closely related to their antioxidant capacities. Antioxidant activities of G. tournefortii, both for the aerial parts and seeds, were investigated by using both 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and lipid peroxidation inhibition methods. The seeds were found to have higher antioxidant potential than the aerial, with IC50 valu...
Anticarcinogenic effects of the ethanolic extract from salix aegyptiaca l. in colon cancer, involvement of akt pathway
Enayat, Shabnam; Banerjee, Sreeparna; Department of Biology (2013)
Salix aegyptiaca has been used for centuries as a medicinal plant for various health disorders. Our previous study demonstrated high contents of phenolics and antioxidants in its bark many of those are famed for their anti-inflammatory properties. Since inflammation frequently accompanies the progress of colorectal cancer, in the current study we showed that the ethanolic extract of bark (EEB) from S.aegyptiaca exerted strong anti-proliferative effects on several cancer cells but not on non-cancerous CCD-18...
Antioxidant and cytotoxic properties of Salvia Absconditiflora and effects on CYP1A1, CYP1B1 gene expressions in breast cancer cell lines
Yılmaz, Selis; İşcan, Mesude; Güray, Tülin; Department of Biochemistry (2013)
Salvia genus is a widely cultivated genus and used in medicine for various purposes as having antimicrobial, antioxidant, anticarcinogen and anti-inflammatory features. In this study the aim was to investigate phenolic composition of Salvia absconditiflora and understand the possible effects of those constituents in cancer related drug metabolizing enzymes. Salvia absconditiflora showed 80,43 % Radical Scavenging Activity against DPPH radical. Total flavonoid content was found as one third of total phenolic...
Citation Formats
S. Enayat, M. S. Ceyhan, A. A. BAŞARAN, M. Gürsel, and S. Banerjee, “Anticarcinogenic Effects of the Ethanolic Extract of Salix aegyptiaca in Colon Cancer Cells: Involvement of Akt/PKB and MAPK Pathways,” NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, pp. 1045–1058, 2013, Accessed: 00, 2020. [Online]. Available: