MicroRNAs show a wide diversity of expression profiles in the developing and mature central nervous system

Since the discovery of microRNAs (miRNAs) in Caenorhabditis elegans, mounting evidence illustrates the important regulatory roles for miRNAs in various developmental, differentiation, cell proliferation, and apoptosis pathways of diverse organisms. We are just beginning to elucidate novel aspects of RNA mediated gene regulation and to understand how heavily various molecular pathways rely on miRNAs for their normal function. miRNAs are small non-protein-coding transcripts that regulate gene expression post-transcriptionally by targeting messenger RNAs (mRNAs). While individual miRNAs have been specifically linked to critical developmental pathways, the deregulated expression of many miRNAs also has been shown to have functional significance for multiple human diseases, such as cancer. We continue to discover novel functional roles for miRNAs at a rapid pace. Here, we summarize some of the key recent findings on miRNAs, their mode of action, and their roles in both normal development and in human pathology. A better understanding of how miRNAs operate during the normal life of a cell as well as in the pathogenesis of disease when deregulated will provide new avenues for diagnostic, prognostic, and therapeutic applications.


Intergenic and Repeat Transcription in Human, Chimpanzee and Macaque Brains Measured by RNA-Seq
Xu, Augix Guohua; He, Liu; Li, Zhongshan; Xu, Ying; Li, Mingfeng; Fu, Xing; Yan, Zheng; Yuan, Yuan; Menzel, Corinna; Li, Na; Somel, Mehmet; Hu, Hao; Chen, Wei; Paabo, Svante; Khaitovich, Philipp (Public Library of Science (PLoS), 2010-07-01)
Transcription is the first step connecting genetic information with an organism's phenotype. While expression of annotated genes in the human brain has been characterized extensively, our knowledge about the scope and the conservation of transcripts located outside of the known genes' boundaries is limited. Here, we use high-throughput transcriptome sequencing (RNA-Seq) to characterize the total non-ribosomal transcriptome of human, chimpanzee, and rhesus macaque brain. In all species, only 20-28% of non-ri...
RNA-biology ruling cancer progression? Focus on 3 ' UTRs and splicing
Erson Bensan, Ayşe Elif (Springer Science and Business Media LLC, 2020-09-01)
The protein-coding regions of mRNAs have the information to make proteins and hence have been at the center of attention for understanding altered protein functions in disease states, including cancer. Indeed, the discovery of genomic alterations and driver mutations that change protein levels and/or activity has been pivotal in our understanding of cancer biology. However, to better understand complex molecular mechanisms that are deregulated in cancers, we also need to look at non-coding parts of mRNAs, i...
APOBEC3B expression in drug resistant MCF-7 breast cancer cell lines
Onguru, Onder; Yalcin, Serap; Rosemblit, Cinthia; Zhang, Paul J.; Kilic, Selim; Gündüz, Ufuk (2016-04-01)
APOBEC3B belongs to a protein family of cytidine deaminases that can insert mutations in DNA and RNA as a result of their ability to deaminate cytidine to uridine. It has been shown that APOBEC3B-catalysed deamination provides a chronic source of DNA damage in breast cancers. We investigated APOBEC3B expression in four drug resistant breast cancer cell lines (Doxorubicin, Etoposide, Paclitaxel and Docetaxel resistant MCF-7 cell lines) using a novel RNA in situ hybridization technology (RNAscope) and compare...
Functional characterization of microrna-125b expression in MCF7 breast cancer cell line
Tuna, Serkan; Erson Bensan, Ayşe Elif; Department of Biology (2010)
microRNA dependent gene expression regulation has roles in diverse processes such as differentiation, proliferation and apoptosis. Therefore, deregulated miRNA expression has functional importance for various diseases, including cancer. miR-125b is among the commonly downregulated miRNAs in breast cancer cells . Therefore we aimed to characterize the effects of miR-125b expression in MCF7 breast cancer cell line (BCCL) to better understand its roles in tumorigenesis. Here, we investigated mir-125 family mem...
Abiotic stress tolerance and growth responses of transgenic potato (Solanum tuberosum L. cv. Kennebec) plants expressing rice Osmyb4 gene
AYDIN, GÜLSÜM; Yucel, Meral; Öktem, Hüseyin Avni (2012-09-23)
MYB transcription factors are involved in diverse biochemical and physiological processes such as regulation of secondary metabolism, meristem formation, cell morphogenesis and floral and seed development. They are also involved in certain defence and stress responses and in hormone signalling. In the present study, we developed transgenic potato (Solanum tuberosum L. cv. Kennebec) expressing Oryza sativa myb4 gene, encoding MYB4 transcription factor, driven by either CaMV35S constitutive promoter or cold i...
Citation Formats
A. E. Erson Bensan, “MicroRNAs show a wide diversity of expression profiles in the developing and mature central nervous system,” CLINICAL GENETICS, pp. 296–306, 2008, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/43542.