Dielectric Characterization of Imatinib Resistant K562 Leukemia Cells through Electrorotation with 3-D Electrodes

Bahrieh, G.
Koydemir, H. Ceylan
Erdem, Murat
Ozgur, E.
Gündüz, Ufuk
Külah, Haluk
This study reports the use of electrorotation (ER) technique within a micro-fabricated device with 3D electrodes for dielectric characterization of Imatinib-resistant K562 (K562/IMA-0.2) human leukemic cells. The ER devices with 3D quadruple electrodes (30 mu m in height) were used in order to eliminate the fringing field effect on the rotation of cells. To induce the rotational moment on the cells, signals in phase quadrature were applied to the triangular electrodes. The rotation of cells measured at the frequency range of 1 kHz to 0.1 MHz in various medium conductivities. The membrane effective conductance, membrane capacitance, and the inner conductivity of cells were measured as 2953 +/- 82 [Sm-2], 15.63 +/- 3.02 [mFm(-2)], and 0.60 +/- 0.10 [Sm-1], respectively. These results can be used in the designing and implementation of MEMS based DEP devices for separation and detection of imatinib resistant K562 cells.


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Dielectrophoretic detection of imatinib resistance in K562 Cells Using A lab-on-a-chip system
Demircan Yalçın, Yağmur; Toral, Taylan Berkın; Sukaş, Serkan; Yıldırım, Ender; Zorlu, Özge; Gündüz, Ufuk; Külah, Haluk (Chemical and Biological Microsystems Society (CBMS); 2019-10-27)
This study presents label-free detection of imatinib resistance in K562 cells via integrated dielectrophoretic detection and impedimetric counting on a lab-on-a-chip system. Two impedimetric counting units were placed upstream and downstream of dielectrophoretic detection unit to obtain differential cell count and consequently the trapping ratio. The trapping ratio up to 57% was achieved for imatinib resistant (~60-fold) K562 cells, while it was minimized to 20% for wild type K562 cells
Citation Formats
G. Bahrieh, H. C. Koydemir, M. Erdem, E. Ozgur, U. Gündüz, and H. Külah, “Dielectric Characterization of Imatinib Resistant K562 Leukemia Cells through Electrorotation with 3-D Electrodes,” 2013, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/53984.