Impact of wastewater treatment plants on dissemination of antibiotic resistance genes

Download

index.pdf

Date

2019

Author

Küçükünsal, Serkan

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

357
views

169
downloads

Wastewater treatment plants (WWTPs) as hotspots of the antibiotic resistance genes (ARGs) pose risk to receiving environments. Removal of ARGs through WWTPs, therefore, is great importance. For this reason, common types of WWTPs including conventional activated sludge (CAS), biological nutrient removal (BNR), sequencing batch reactor (SBR), membrane bioreactor (MBR), package MBR, WWTP with coagulation-flocculation and UV disinfection units were investigated in terms of their seasonal removal efficiencies on seven ARGs including the genes blaCTX-M, sul1, tetA, aadA, ermB, qnrS and cmlA corresponding to commonly used antibiotics β-lactams, sulfonamides - trimethoprim, tetracyclines, aminoglycosides, macrolides – lincosamides - streptogramins, quinolones and amphenicols, respectively. Copy numbers of the ARGs and overall bacterial genes in both influents and effluents of the each WWTP were determined through quantitative polymerase chain reaction. The highest ARG removal efficiencies were obtained from MBR and package MBR systems. Significant seasonal variations were observed in the CAS and BNR systems (p<0.05). The lowest log reductions were determined in both autumn and winter for CAS and in summer for BNR systems. The abundance of the overall bacterial genes were elevated in the sludge of CAS system. Similarities in the removal efficiencies of all WWTPs tested on both overall genes and ARGs indicated that removal mechanism was not ARG specific.

Subject Keywords

Pollutants., ARG, WWTP, Antibiotic Resistance, ARG Removal.

URI

http://etd.lib.metu.edu.tr/upload/12623697/index.pdf
https://hdl.handle.net/11511/44094

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

Impact of UV treatment for the removal of bacterial genes during wastewater treatment Bulut, Taliye; İçgen, Bülent (null; 2018-06-23) Wastewater treatment plants (WWTPs) are considered as important hotspots for the spread of the antibiotic resistance genes (ARGs). The dissemination of ARGs is one of the most significant threat to public health. This also causes a danger for water quality in surface waters and groundwater. These waters can easily be included in human life by their use in places such as agriculture, livestock and drinking waters. The bacterial gene is important to analyze the quantity of the total bacterial load and to norm...
Effect of UV treatment for the removal of sulfonamide resistance gene during wastewater treatment Bulut, Taliye; İçgen, Bülent (2018-04-27) The dissemination of the antibiotic resistance genes (ARGs) is considered as one of the most important threats to public health. This dissemination also becomes a threat for water quality in surface waters and groundwater. Waters contaminated with ARGs can easily be incorporated into human life through several ways like drinking water, agriculture and animal husbandry. Sulfonamide resistance gene, one of the ARGs, in discharged waters increases gradually because the use of these drugs is highly common. Wast...
Impact of different environmental conditions on the aggregation of biogenic U(IV) nanoparticles synthesized by Desulfovibrio alaskensis G20 Şengör, Sema Sevinç; Dohnalkova, Alice; Spycher, Nicolas; Ginn, Timothy R.; Peyton, Brent M.; Sani, Rajesh K. (2016-12-01) This study investigates the impact of specific environmental conditions on the formation of colloidal U(IV) nanoparticles by the sulfate reducing bacteria (SRB, Desulfovibrio alaskensis G20). The reduction of soluble U(VI) to less soluble U(IV) was quantitatively investigated under growth and non-growth conditions in bicarbonate or 1,4-piperazinediethanesulfonic acid (PIPES) buffered environments. The results showed that under non-growth conditions, the majority of the reduced U nanoparticles aggregated and...
Removal of antibiotic resistance genes in various water resources recovery facilities Kucukunsal, Serkan; İçgen, Bülent (Wiley, 2020-06-01) Water resources recovery facilities (WWTPs) are hotspots for antibiotic resistance genes (ARGs) and pose a significant threat to environments. Therefore, ARG removal efficiencies of WWTPs are of great importance. In this study, conventional activated sludge (CAS), biological nutrient removal (BNR), sequencing batch reactor (SBR), membrane bioreactor (MBR), package MBR, and WWTP with coagulation-flocculation and UV disinfection units were investigated in terms of their removal efficiencies on overall bacteri...
The effects of palbociclib on 2d and 3d cell cultures and doxorubicin resistance Yalçın, Gizem Damla; Gündüz, Ufuk; Department of Molecular Biology and Genetics (2020) Cell culture facilitates studying the mechanism of diseases and finding new treatment strategies. Traditionally used twodimensional cell culture model is not quite sufficient in some studies due to the factors such as lack of cell to cell and cellextracellular matrix interactions and changing cell morphology. Because of the limitations in 2D cell culture, nowadays threedimensional cell cultures gain importance. The present study aims to identify the effect of Palbociclib on different cells when they cult...

Citation Formats

S. Küçükünsal, “Impact of wastewater treatment plants on dissemination of antibiotic resistance genes,” Thesis (M.S.) -- Graduate School of Natural and Applied Sciences. Environmental Engineering., Middle East Technical University, 2019.