Show/Hide Menu
Hide/Show Apps
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Open Science Policy
Open Science Policy
Open Access Guideline
Open Access Guideline
Postgraduate Thesis Guideline
Postgraduate Thesis Guideline
Communities & Collections
Communities & Collections
Help
Help
Frequently Asked Questions
Frequently Asked Questions
Guides
Guides
Thesis submission
Thesis submission
MS without thesis term project submission
MS without thesis term project submission
Publication submission with DOI
Publication submission with DOI
Publication submission
Publication submission
Supporting Information
Supporting Information
General Information
General Information
Copyright, Embargo and License
Copyright, Embargo and License
Contact us
Contact us
Impact of wastewater treatment plants on dissemination of antibiotic resistance genes
Download
index.pdf
Date
2019
Author
Küçükünsal, Serkan
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
347
views
166
downloads
Cite This
Wastewater treatment plants (WWTPs) as hotspots of the antibiotic resistance genes (ARGs) pose risk to receiving environments. Removal of ARGs through WWTPs, therefore, is great importance. For this reason, common types of WWTPs including conventional activated sludge (CAS), biological nutrient removal (BNR), sequencing batch reactor (SBR), membrane bioreactor (MBR), package MBR, WWTP with coagulation-flocculation and UV disinfection units were investigated in terms of their seasonal removal efficiencies on seven ARGs including the genes blaCTX-M, sul1, tetA, aadA, ermB, qnrS and cmlA corresponding to commonly used antibiotics β-lactams, sulfonamides - trimethoprim, tetracyclines, aminoglycosides, macrolides – lincosamides - streptogramins, quinolones and amphenicols, respectively. Copy numbers of the ARGs and overall bacterial genes in both influents and effluents of the each WWTP were determined through quantitative polymerase chain reaction. The highest ARG removal efficiencies were obtained from MBR and package MBR systems. Significant seasonal variations were observed in the CAS and BNR systems (p<0.05). The lowest log reductions were determined in both autumn and winter for CAS and in summer for BNR systems. The abundance of the overall bacterial genes were elevated in the sludge of CAS system. Similarities in the removal efficiencies of all WWTPs tested on both overall genes and ARGs indicated that removal mechanism was not ARG specific.
Subject Keywords
Pollutants.
,
ARG
,
WWTP
,
Antibiotic Resistance
,
ARG Removal.
URI
http://etd.lib.metu.edu.tr/upload/12623697/index.pdf
https://hdl.handle.net/11511/44094
Collections
Graduate School of Natural and Applied Sciences, Thesis
Suggestions
OpenMETU
Core
Impact of UV treatment for the removal of bacterial genes during wastewater treatment
Bulut, Taliye; İçgen, Bülent (null; 2018-06-23)
Wastewater treatment plants (WWTPs) are considered as important hotspots for the spread of the antibiotic resistance genes (ARGs). The dissemination of ARGs is one of the most significant threat to public health. This also causes a danger for water quality in surface waters and groundwater. These waters can easily be included in human life by their use in places such as agriculture, livestock and drinking waters. The bacterial gene is important to analyze the quantity of the total bacterial load and to norm...
Effect of UV treatment for the removal of sulfonamide resistance gene during wastewater treatment
Bulut, Taliye; İçgen, Bülent (2018-04-27)
The dissemination of the antibiotic resistance genes (ARGs) is considered as one of the most important threats to public health. This dissemination also becomes a threat for water quality in surface waters and groundwater. Waters contaminated with ARGs can easily be incorporated into human life through several ways like drinking water, agriculture and animal husbandry. Sulfonamide resistance gene, one of the ARGs, in discharged waters increases gradually because the use of these drugs is highly common. Wast...
Impact of different environmental conditions on the aggregation of biogenic U(IV) nanoparticles synthesized by Desulfovibrio alaskensis G20
Şengör, Sema Sevinç; Dohnalkova, Alice; Spycher, Nicolas; Ginn, Timothy R.; Peyton, Brent M.; Sani, Rajesh K. (2016-12-01)
This study investigates the impact of specific environmental conditions on the formation of colloidal U(IV) nanoparticles by the sulfate reducing bacteria (SRB, Desulfovibrio alaskensis G20). The reduction of soluble U(VI) to less soluble U(IV) was quantitatively investigated under growth and non-growth conditions in bicarbonate or 1,4-piperazinediethanesulfonic acid (PIPES) buffered environments. The results showed that under non-growth conditions, the majority of the reduced U nanoparticles aggregated and...
Removal of antibiotic resistance genes in various water resources recovery facilities
Kucukunsal, Serkan; İçgen, Bülent (Wiley, 2020-06-01)
Water resources recovery facilities (WWTPs) are hotspots for antibiotic resistance genes (ARGs) and pose a significant threat to environments. Therefore, ARG removal efficiencies of WWTPs are of great importance. In this study, conventional activated sludge (CAS), biological nutrient removal (BNR), sequencing batch reactor (SBR), membrane bioreactor (MBR), package MBR, and WWTP with coagulation-flocculation and UV disinfection units were investigated in terms of their removal efficiencies on overall bacteri...
The effects of palbociclib on 2d and 3d cell cultures and doxorubicin resistance
Yalçın, Gizem Damla; Gündüz, Ufuk; Department of Molecular Biology and Genetics (2020)
Cell culture facilitates studying the mechanism of diseases and finding new treatment strategies. Traditionally used twodimensional cell culture model is not quite sufficient in some studies due to the factors such as lack of cell to cell and cellextracellular matrix interactions and changing cell morphology. Because of the limitations in 2D cell culture, nowadays threedimensional cell cultures gain importance. The present study aims to identify the effect of Palbociclib on different cells when they cult...
Citation Formats
IEEE
ACM
APA
CHICAGO
MLA
BibTeX
S. Küçükünsal, “Impact of wastewater treatment plants on dissemination of antibiotic resistance genes,” Thesis (M.S.) -- Graduate School of Natural and Applied Sciences. Environmental Engineering., Middle East Technical University, 2019.