Impact of different environmental conditions on the aggregation of biogenic U(IV) nanoparticles synthesized by Desulfovibrio alaskensis G20

Date

2016-12-01

Author

Şengör, Sema Sevinç
Dohnalkova, Alice
Spycher, Nicolas
Ginn, Timothy R.
Peyton, Brent M.
Sani, Rajesh K.

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

127
views

6
downloads

This study investigates the impact of specific environmental conditions on the formation of colloidal U(IV) nanoparticles by the sulfate reducing bacteria (SRB, Desulfovibrio alaskensis G20). The reduction of soluble U(VI) to less soluble U(IV) was quantitatively investigated under growth and non-growth conditions in bicarbonate or 1,4-piperazinediethanesulfonic acid (PIPES) buffered environments. The results showed that under non-growth conditions, the majority of the reduced U nanoparticles aggregated and precipitated out of solution. High resolution transmission electron microscopy revealed that only a very small fraction of cells had reduced U precipitates in the periplasmic spaces in the presence of PIPES buffer, whereas in the presence of bicarbonate buffer, reduced U was also observed in the cytoplasm with greater aggregation of biogenic U(IV) particles at higher initial U(VI) concentrations. The same experiments were repeated under growth conditions using two different electron donors (lactate and pyruvate) and three electron acceptors (sulfate, fumarate, and thiosulfate). In contrast to the results of the non-growth experiments, even after 0.2 mu m filtration, the majority of biogenic U(IV) remained in the aqueous phase resulting in potentially mobile biogenic U(IV) nanoparticles. Size fractionation results showed that U(IV) aggregates were between 18 and 200 nm in diameter, and thus could be very mobile. The findings of this study are helpful to assess the size and potential mobility of reduced U nanoparticles under different environmental conditions, and would provide insights on their potential impact affecting U(VI) bioremediation efforts at subsurface contaminated sites.

URI

https://hdl.handle.net/11511/93850

Journal

BIOMETALS

DOI

https://doi.org/10.1007/s10534-016-9969-6

Collections

Department of Environmental Engineering, Article

Suggestions

OpenMETU
Core

Impact of wastewater treatment plants on dissemination of antibiotic resistance genes Küçükünsal, Serkan; İçgen, Bülent; Department of Environmental Engineering (2019) Wastewater treatment plants (WWTPs) as hotspots of the antibiotic resistance genes (ARGs) pose risk to receiving environments. Removal of ARGs through WWTPs, therefore, is great importance. For this reason, common types of WWTPs including conventional activated sludge (CAS), biological nutrient removal (BNR), sequencing batch reactor (SBR), membrane bioreactor (MBR), package MBR, WWTP with coagulation-flocculation and UV disinfection units were investigated in terms of their seasonal removal efficiencies on...
Impact of UV treatment for the removal of bacterial genes during wastewater treatment Bulut, Taliye; İçgen, Bülent (null; 2018-06-23) Wastewater treatment plants (WWTPs) are considered as important hotspots for the spread of the antibiotic resistance genes (ARGs). The dissemination of ARGs is one of the most significant threat to public health. This also causes a danger for water quality in surface waters and groundwater. These waters can easily be included in human life by their use in places such as agriculture, livestock and drinking waters. The bacterial gene is important to analyze the quantity of the total bacterial load and to norm...
Influence of oxygen transfer on benzaldehyde lyase production by recombinant Escherichia coli BL21(DE3) pLySs Angardi, Vahideh; Çalık, Pınar; Department of Chemical Engineering (2007) In this study, the effects of oxygen transfer conditions on the synthesis of the enzyme benzaldehyde lyase as intracellular in recombinant E. coli BL21 (DE3) pLysS was investigated sistematically and a comprehensive model was developed to determine benzaldehyde lyase activity. For this purpose, the research program was carried out in mainly two parts. In the first part of study, the effects of oxygen transfer together with the mass transfer coefficient (KLa), enhancement factor E (=KLa/KLao), volumetric oxy...
Effect of pre-acclimation of granular activated carbon on microbial electrolysis cell startup and performance LaBarge, Nicole; Yılmazel Tokel, Yasemin Dilşad; Hong, Pei-Ying; Logan, Bruce E. (2017-02-01) Microbial electrolysis cells (MECs) can generate methane by fixing carbon dioxide without using expensive catalysts, but the impact of acclimation procedures on subsequent performance has not been investigated. Granular activated carbon (GAC) was used to pre-enrich electrotrophic methanogenic communities, as GAC has been shown to stimulate direct transfer of electrons between different microbial species. MEC startup" times using pre-acclimated GAC were improved compared to controls (without pre-acclimation ...
Effect of nutrients on culture dynamics of marine phytoplankton Eker-Develi, E; Kıdeyş, Ahmet Erkan; TUĞRUL, SÜLEYMAN (2006-01-01) The effects of nitrate, ammonium and phosphate on the abundance, chlorophyll a content (chl a), in vivo fluorescence, particulate organic carbon, nitrogen, phosphorus and cell morphology of the diatom Skeletonema costatum, the dinoflagellate Prorocentrum micans and the coccolithophore Emiliania huxleyi were investigated in the laboratory. The carbon:chlorophyll a ratio (as weight), a parameter often used in productivity estimates, differed substantially among the three species as well as at different nutrie...

Citation Formats

S. S. Şengör, A. Dohnalkova, N. Spycher, T. R. Ginn, B. M. Peyton, and R. K. Sani, “Impact of different environmental conditions on the aggregation of biogenic U(IV) nanoparticles synthesized by Desulfovibrio alaskensis G20,” BIOMETALS, vol. 29, no. 6, pp. 965–980, 2016, Accessed: 00, 2021. [Online]. Available: https://hdl.handle.net/11511/93850.