Design and in vitro testing of a peripheral nerve guide

Download

index.pdf

Date

2019

Author

Tamay, Dilara Göksu

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

222
views

86
downloads

Regeneration after peripheral nerve injury (PNI) is limited as chondroitin sulfate proteoglycans (CSPGs) within the scar tissue inhibit axon outgrowth. Nerve guides (NGs) are alternatives to the “gold standard” autografting, which has limitations including donor site morbidity and limited tissue availability. NGs are tubular constructs designed to form a bridge between the transected nerve ends, provide physical support, and achieve axon guidance via topographical and/or chemical cues. This study aims to design and test the in vitro performance of a NG with topographical cues, capable of removing CSPGs via release of enzyme chondroitinase ABC (ChABC). A bisphenol A methacrylate (Dental SG)-based NG filled with crosslinked gelatin methacrylate (GelMA) hydrogel containing microchannels was constructed. For practical observation, a halved tube was used for in vitro testing. Cell adhesion and viability analyses were conducted in vitro using neuron-like PC12 cell line. Optimum GelMA concentration was determined as 15% (w/v). In vitro studies showed hydrogel provides cells with the optimal conditions for attachment and growth. Enzyme release from alginate (using BSA as a model) was in accordance to Korsmeyer-Peppas model with a 100% release achieved in ~3 days. 3D printed Dental SG exterior was shown to significantly degrade in the presence of cells. The effectiveness of NG in supporting cell viability and growth was tested in vitro. Cells showed good attachment and were viable along the microchannels with visible alignment during 3 days of incubation. However, due to possible toxic effect of Dental SG degradation products, cells did not survive until Day 7.

Subject Keywords

Heparin., Nerve guide; 3D printing; GelMA; chondroitinase ABC

URI

http://etd.lib.metu.edu.tr/upload/12624126/index.pdf
https://hdl.handle.net/11511/44361

Collections

Graduate School of Natural and Applied Sciences, Thesis

Suggestions

OpenMETU
Core

DEVELOPMENT OF A COMPOSITE GUIDE FOR PERIPHERAL NERVE REGENERATION Arslantunalı Şahin, Damla; Son, Çağdaş Devrim; Hasırcı, Vasıf Nejat; Department of Biotechnology (2022-2-1) Any injury in peripheral nerves may result in a loss of neuronal communication along sensory and motor nerves, affecting patients’ daily activities. Today, there are various FDA approved commercial conduit materials; hollow tubes preventing them from used in gaps bigger than 10 mm, because they may lead axonal sprouts to form. The presented study includes pHEMA wrapping structure filled with GelMA-HaMA gel matrix as a nerve guidance channel. Following the structural analysis of the nerve guide, in vitro stu...
Multiwalled CNT-pHEMA composite conduit for peripheral nerve repair. Arslantunalı Şahin, Damla; Hasırcı, Vasıf Nejat (2014-03-01) A nerve conduit is designed to improve peripheral nerve regeneration by providing guidance to the nerve cells. Conductivity of such guides is reported to enhance this process. In the current study, a nerve guide was constructed from poly(2-hydroxyethyl methacrylate) (pHEMA), which was loaded with multiwalled carbon nanotubes (mwCNT) to introduce conductivity. PHEMA hydrogels were designed to have a porous structure to facilitate the transportation of the compounds needed for cell nutrition and growth and al...
Multiwalled Carbon Nanotube- Poly(2-Hydroxyethyl Methacrylate) Composite Conduitfor Peripheral Nerve Repair Arslantunalı, Damla; Arslantunalı Şahin, Damla; Department of Biotechnology (2012) There are different methods used in the surgical treatment of peripheral nerve injury. In this respect, end-to-end surgical reconnection of the damaged nerve ends or autologous nerve grafts are applied as soon as possible after the injury. When autologous tissue transplant is considered, there are some medical devices available generally for relatively short nerve defects. As a solution for this problem, different tissue engineered nerve conduits have been developed. In the current study, a pHEMA hydrogel m...
Characterization and Evaluation of Triamcinolone, Raloxifene, and Their Dual-Loaded Microspheres as Prospective Local Treatment System in Rheumatic Rat Joints Ocal, Yigit; Kurum, Baris; Karahan, Siyami; Tezcaner, Ayşen; Ozen, Seza; Keskin, Dilek (Elsevier BV, 2014-8) In this study, injectable microspheres were developed for the local treatment of joint degeneration in rheumatoid arthritis (RA). Microspheres loaded with triamcinolone (TA), a corticosteroid drug, and/or raloxifene (Ral), a cartilage regenerative drug, were prepared with a biodegradable and biocompatible polymer, polycaprolactone (PCL). Microspheres were optimized for particle size, structural properties, drug release, and loading properties. In vitro release of Ral was very slow because of the low solubil...
Differentiation of BMSCs into Nerve Precursor Cells on Fiber-Foam Constructs for Peripheral Nerve Tissue Engineering Dursun Usal, Tuğba; YÜCEL, DENİZ; Hasırcı, Vasıf Nejat (2018-06-01) Bone marrow stem cells (BMSCs) are frequently used in nerve tissue engineering studies due to ease of their isolation and high potential for differentiation into nerve cells. A bilayer fiber-foam construct containing nanofibrous elements to house and guide BMSCs was designed as a model to study the regeneration of damaged peripheral nerve tissue and eventually serve as a nerve guide. The construct consisted of a) a macroporous bottom layer to serve as the backing and support, and for nutrient transport, and...

Citation Formats

D. G. Tamay, “Design and in vitro testing of a peripheral nerve guide,” Thesis (M.S.) -- Graduate School of Natural and Applied Sciences. Biotechnology., Middle East Technical University, 2019.