Bacterial cellulase production using grape pomace hydrolysate and synthetic sugar as sole carbon sources by shake-flask submerged fermentation

Kurt, Ayşe Sultan
Grape pomace is the major waste in the wine industry and it consists of high moisture content and residual sugars, which make it susceptible to rapid microbial spoilage. Thus, it needs to be disposed of with care to eliminate both environmental and health problems. The carbohydrate fraction of grape pomace is a fibrous material, and is an additional source of fermentable sugars to produce biofuel and hydrolytic enzymes. In this study, grape pomace hydrolysate was used for cellulase production with Bacillus subtilis Natto DSM 17766 at 37 °C and 130 rpm using batch fermentation method. Cellulase production was optimized for solid loading of grape pomace, pH, and fermentation period by Box-Behnken response surface method. Furthermore, cellulase was produced by Bacillus subtilis NRRL B-4219 using monosaccharides which were mixed to obtain similar sugar content with grape pomace. Cellulase activity from both carbon sources were assayed by the DNS method using filter paper as substrate. The maximum cellulase activity was obtained as 0.48 IU/mL from experiments conducted by synthetic sugar, which has the monosaccharide combination of glucose, fructose, xylose, arabinose, mannose, and galactose. In contrast, the highest cellulase activity using grape pomace was achieved at 0.196 IU/mL with 12.5% of solid loading at pH 7.0 on 5th day. The quadratic response surface model predicted an optimal cellulase activity of 0.178 IU/mL with 15% solid loading and pH 6.0 on 7th day. These results indicate that grape pomace is a potential carbon source for bacterial cellulase production.


Improvement of Pectinase Production by Co-culture of Bacillus spp. Using Apple Pomace as a Carbon Source
Kuvvet, Ceren; UZUNER, SİBEL; Çekmecelioğlu, Deniz (2019-05-01)
Increasing interest in using food and agricultural wastes for low cost enzyme production continues in fermentation industry. Bacteria, especially Bacillus subtilis, are known to produce pectinases in synthetic media and media amended with agricultural wastes as a carbon source, whereas the potential of co-culturing Bacillus species to produce pectinases has not been fully studied yet. In this study, co-culture of B. subtilis and B. pumilus was optimized for solid load and pH to maximize pectinase production...
Bacterial and fungal strain selections for cellulase and xylanase production using distillers' dried grains with solubles (DDGS)
Iram, Attia; Çekmecelioğlu, Deniz; Demirci, Ali (2020-01-01)
Distillers' dried grains with solubles (DDGS) are by-products of dry-mill bioethanol production from corn and wheat. The DDGS is leftover by the yeast after converting the starch into ethanol and it has a high nutritional profile including undigested fiber and protein fractions. The DDGS can be used as cheap microbial feedstock for the production of cellulase and xylanases, which are produced from expensive industrial feedstocks. In this study, screening of several bacterial and fungal strains was done usin...
Pectinase production using apple pomace as carbon source by mixed culture fermentation
Kuvvet, Ceren; Çekmecelioğlu, Deniz; Department of Biotechnology (2016)
There is a growing interest in enzyme production from food and agricultural wastes to reduce the cost of production. Pectinases are one of the important group of enzymes used in fruit processing, alcoholic beverages, coffee and tea fermentation and textile industries. Pectinases can be produced by both solid state and submerged fermentation from various plant and microorganisms; however fungal microorganisms are the largest sources of bulk commercial enzymes and there are numerous studies on pectinase produ...
Optimization of dilute sulfuric acid, aqueous ammonia, and steam explosion as the pretreatments steps for distillers' dried grains with solubles as a potential fermentation feedstock
Iram, Attia; Çekmecelioğlu, Deniz; Demirci, Ali (Elsevier BV, 2019-06-01)
Distillers' dried grains with solubles (DDGS) is the by-product of bioethanol production from starch-rich grains through dry-mill fermentation. In this study, dilute sulfuric acid hydrolysis, aqueous ammonia, and steam explosion as the pre-treatment methods were optimized. The central composite response surface methodology (RSM) design was used for optimization of dilute acid pretreatment, aqueous ammonia pretreatment. The steam explosion trials were evaluated. The results show that the dilute acid pretreat...
Polyphenol content in selected Turkish wines, an alternative method of detection of phenolics
Kiralp, S; Toppare, Levent Kamil (Elsevier BV, 2006-01-01)
Wine is an important source of dietary antioxidants, due to its content of phenolic compounds. Amount of phenolics in red wines of Turkey were investigated by using electrodes prepared via immobilisation of tyrosinase. Immobilisation was performed by entrapment during electrochemical synthesis of conducting copolymers. Results were compared with Folin-Ciocalteau method. It was found that use of enzyme electrodes is an alternative and cheap method in determining the phenolics in wines. Storage temperature be...
Citation Formats
A. S. Kurt, “Bacterial cellulase production using grape pomace hydrolysate and synthetic sugar as sole carbon sources by shake-flask submerged fermentation,” Thesis (M.S.) -- Graduate School of Natural and Applied Sciences. Food Engineering., Middle East Technical University, 2019.