Behavior of neuro-2A cells on femtosecond laser structured silicon substrates

Mingu, Sara
Cells are known to interact with their physical environment and respond to cues such as substrate topography. Knowledge of the cell responses to topography may give information about cell behavior in health and disease, as well as be exploited in order to exert control on cells for various purposes. Cell responses to topography are dependent on cell type, substrate material and topographical features. In the present study, Neuro-2a cell line was used as a versatile and widely available neuronal cell model. The substrates consisted of polished or laser-structured silicon. Structuring was performed using an ultrafast infrared pulsed laser, which generated topographies such as laser induced periodic surface structures (LIPSS) and microcolumns. The substrates were characterized with scanning electron microscopy (SEM). Cells were grown on control substrates (glass or plastic), polished silicon and laser-structured silicon of different topographies for 3 hours or 24 hours to evaluate various cell behaviors. Initial cell adhesion and initial motility, as well as cell adhesion and shape after 24 hours were studied in different substrates using fluorescence microscopy and SEM. Initial cell adhesion was found to be strongest on the microcolumn topography, allowing for v selective cell patterning on microcolumn regions. After 24 hours, cell adhesion was found to be equal in all topographies. Moreover, cell motility was found to be fastest in polished silicon, and slowest in the microcolumn topography. On the other hand, cell area and perimeter was found to be larger on polished silicon and LIPSS, compared to microcolumn topography or glass. No difference was found in the average cell circularity for all substrates. Finally, cell preference for microcolumn stripes was found to be more prominent when LIPSS was found between the stripes, compared to when thee stripes were separated by flat regions. In conclusion, different cell behaviors related to spreading, migration and adhesion were found to be dependent on the substrate topography. Topography on silicon may be promising to control these behaviors in the Neuro-2A cell line.


Culfa, Şeyda; Son, Çağdaş Devrim; Bek, Alpan; Department of Biochemistry (2022-8-29)
Cells' interaction with their environment and how their responses to different mechanical cues are regulated have attracted attention for decades. These mechanical cues vary from topography to substrate stiffness. Knowing how topography-induced cell responses are regulated might give useful information about cell behaviors in health and disease states. This knowledge can be utilized to manipulate cell behaviors for different purposes. Topography-dependent cell behaviors rely on various parameters such as ce...
A high throughput approach for analysis of cell nuclear deformability at single cell level
Ermis, Menekse; Akkaynak, Derya; Chen, Pu; Demirci, Utkan; Hasırcı, Vasıf Nejat (2016-11-14)
Various physiological and pathological processes, such as cell differentiation, migration, attachment, and metastasis are highly dependent on nuclear elasticity. Nuclear morphology directly reflects the elasticity of the nucleus. We propose that quantification of changes in nuclear morphology on surfaces with defined topography will enable us to assess nuclear elasticity and deformability. Here, we used soft lithography techniques to produce 3 dimensional (3-D) cell culture substrates decorated with micron ...
The Behaviour of Neuro-2A Cells on Silicon Substrates with Various Topographies Generated by Femtosecond Laser Micromachining
Mingu, Sara; Pavlov, Ihor; Son, Çağdaş; Bek, Alpan (SCITEPRESS; 2020-2-24)
The interaction of neural cells with silicon surfaces is important for basic research as well as for various possible applications, such as silicon-based neural implants and neurochips. Laser structuring of silicon provides a quick and versatile method for the generation of complex, hierarchical topographies on precise locations of the substrate. The behaviour ofNeuro-2A cells with laser-structured silicon substrates was studied using a live-imaging setup with fluorescence microscopy. Neuro-2A cells were ab...
Role of Vibrational Spectroscopy in Stem Cell Research
Aksoy, Ceren; Severcan, Feride (2012-01-01)
Recent researches have mainly displayed the significant role of stem cells in tissue renewal and homeostasis with their unique capacity to develop different cell types. These findings have clarified the importance of stem cells to improve the effectiveness of any cell therapy for regenerative medicine. Identification of purity and differentiation stages of stem cells are the greatest challenges of stem cell biology and regenerative medicine. The existing methods to carefully monitor and characterize the ste...
The Effect of physical properties of the ELP-collagen based patterned surfaces on cell attachment and deformation
Antmen, Ezgi; Hasırcı, Vasıf Nejat; Demirci, Utkan; Department of Biology (2013)
Cell and substrate interactions are important in tissue engineering products especially on the behavior of the cells such as adhesion, migration, proliferation, and differentiation. These have been widely studied using substrates with different physical, chemical, and mechanical properties and form. In this study, elastin-like recombinamers (ELRs) were used blended with collagen or only collagen as the surface material. The ELR used in this study has Valine-Proline-Glycine-X-Glycine aminoacid sequences in i...
Citation Formats
S. Mingu, “Behavior of neuro-2A cells on femtosecond laser structured silicon substrates,” Thesis (M.S.) -- Graduate School of Natural and Applied Sciences. Micro and Nanotechnology., Middle East Technical University, 2020.