Culfa, Şeyda
Cells' interaction with their environment and how their responses to different mechanical cues are regulated have attracted attention for decades. These mechanical cues vary from topography to substrate stiffness. Knowing how topography-induced cell responses are regulated might give useful information about cell behaviors in health and disease states. This knowledge can be utilized to manipulate cell behaviors for different purposes. Topography-dependent cell behaviors rely on various parameters such as cell type, substrate material type, and topographical features. The Neuro-2A (N2a) cell line was used in the present study because of its availability and versatility. Two different silicon substrate types, polished or laserfabricated, were used. Fabrication of Silicon was carried out by ultrafast infrared pulsed laser to create micro- and/or nano-topographies. Scanning Electron Microscopy (SEM) and Atomic Force Microscopy were used to characterize substrates. Cells were grown on a control substrate (plastic dish - 35 mm x 10 mm TC- treated plastic cell culture dish which is made up of polystyrene), polished silicon, and laser-fabricated substrates with different topographies for 24 hours to evaluate cell behaviors. After 24 hours, cell morphological responses were studied with confocal microscopy and SEM. Also, after 24 hours, focal-adhesion-related gene expression levels were measured with qRT-PCR and protein expression levels with Western Blot analysis. On all topographies, there was a significant increase in the majority of focal adhesion-related gene expression levels compared to control cells grown on the plastic dish. It was also observed that actin fibers were aligned with the direction of the lines of topographies, especially when two different topographies were combined on a single substrate. When the cells are differentiated, this directional alignment of actin fibers becomes more prominent.In conclusion, substrate topography did not affect only the cells' morphological behaviors but also had a critical role in the changes at the gene and protein levels. Also, it was found that substrate topography has different effects on different cell behaviors associated with spreading and adhesion. Therefore, manipulating these behaviors at the gene and protein levels might be a promising approach for studying cell behaviors in health and disease states in future studies.


The Behaviour of Neuro-2A Cells on Silicon Substrates with Various Topographies Generated by Femtosecond Laser Micromachining
Mingu, Sara; Pavlov, Ihor; Son, Çağdaş; Bek, Alpan (SCITEPRESS; 2020-2-24)
The interaction of neural cells with silicon surfaces is important for basic research as well as for various possible applications, such as silicon-based neural implants and neurochips. Laser structuring of silicon provides a quick and versatile method for the generation of complex, hierarchical topographies on precise locations of the substrate. The behaviour ofNeuro-2A cells with laser-structured silicon substrates was studied using a live-imaging setup with fluorescence microscopy. Neuro-2A cells were ab...
Behavior of neuro-2A cells on femtosecond laser structured silicon substrates
Mingu, Sara; Bek, Alpan; Department of Micro and Nanotechnology (2020)
Cells are known to interact with their physical environment and respond to cues such as substrate topography. Knowledge of the cell responses to topography may give information about cell behavior in health and disease, as well as be exploited in order to exert control on cells for various purposes. Cell responses to topography are dependent on cell type, substrate material and topographical features. In the present study, Neuro-2a cell line was used as a versatile and widely available neuronal cell model. ...
The influence of elastin-like recombinant polymer on the self-renewing potential of a 3D tissue equivalent derived from human lamina propria fibroblasts and oral epithelial cells
Kinikoglu, Beste; Carlos Rodriguez-Cabello, Jose; Damour, Odile; Hasırcı, Vasıf Nejat (2011-09-01)
Three-dimensional epithelial tissue equivalents tend to lose their self-renewing potential progressively during culture as their epithelial cells lose their proliferative capacity with time. Even though the tissue engineered construct can mimic the native tissue well, it rapidly degrades after implantation due to the insufficient number of proliferating cells in the equivalent. In the present study we demonstrate for the first time that the use of an elastin-like recombinant polymer (ELR) engineered to cont...
A Structural Perspective on the Modulation of Protein-Protein Interactions with Small Molecules.
Demirel, Habibe Cansu; Dogan, Tunca; Tunçbağ, Nurcan (2018-01-01)
Protein-Protein Interactions (PPIs) are the key components in many cellular processes including signaling pathways, enzymatic reactions and epigenetic regulation. Abnormal interactions of some proteins may be pathogenic and cause various disorders including cancer and neurodegenerative diseases. Although inhibiting PPIs with small molecules is a challenging task, it gained an increasing interest because of its strong potential for drug discovery and design. The knowledge of the interface as well as the stru...
A comparative study of anisotropic hyperelastic models of biological soft tissues
Açan, Alp Kağan; Dal, Hüsnü; Department of Mechanical Engineering (2021-8)
In the last two decades, there has been significant growth of interest in the mechanical behavior of biological soft tissues approached from the continuum mechanical perspective. A plenty of constitutive models have been proposed that represent the anisotropic hyperelastic behavior of biological soft tissues. Generally, invariant and fiber dispersion-based models are two main categories considered during the modeling steps. Among the anisotropic models, fiber dispersion-based constitutive models are known t...
Citation Formats