Show/Hide Menu
Hide/Show Apps
anonymousUser
Logout
Türkçe
Türkçe
Search
Search
Login
Login
OpenMETU
OpenMETU
About
About
Açık Bilim Politikası
Açık Bilim Politikası
Frequently Asked Questions
Frequently Asked Questions
Browse
Browse
By Issue Date
By Issue Date
Authors
Authors
Titles
Titles
Subjects
Subjects
Communities & Collections
Communities & Collections
New Generation of Solid-Phase Microextraction Coatings for Complementary Separation Approaches: A Step toward Comprehensive Metabolomics and Multiresidue Analyses in Complex Matrices
Download
index.pdf
Date
2017-04-04
Author
Gionfriddo, Emanuela
Boyacı, Ezel
Pawliszyn, Janusz
Metadata
Show full item record
This work is licensed under a
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
.
Item Usage Stats
2
views
2
downloads
In this work, a new generation of solid-phase microextraction (SPME) coatings based on polytetrafluoro-ethylene amorphous fluoroplastics (PTFE AF 2400) as a particle binder is presented. The developed coating was tested for thermal and solvent-assisted desorption, demonstrating its compatibility with both gas- and liquid-chromatographic platforms. The incorporation of hydrophilic-lipophilic balance (HLB) adsorptive particles provided optimal extraction coverage for analytes bearing a broad range of hydrophobicities and molecular weights and of varied chemical diversity. The performance of the newly developed coating was compared to already established coatings based on different polymers such as divinylb enzene/carboxen/polydimethylsiloxane (DVB/Car/ PDMS) and octadecyl/benzenesulfonic acid/polyacrylonitrile (C18/SCX/PAN) in order to assess the new prototype versus the existing technology. As this is the first documented instance of PTFE AF being used as a particle immobilizer for SPME, an assessment of the analyte uptake rate and extraction capability of the developed coating was carried out in comparison to other conventionally used polymers. Moreover, the new SPME probes were used to validate an analytical method for deteimination of banned doping substances, achieving limits of quantitation below the minimum required performance limits (MRPLs) set by the World Anti-Doping Agency (WADA) for most compounds. Considering the broad coverage of the coating in terms of analytes extracted and its suitability for both thermal- and solvent assisted desorption, these new SPME probes will properly suit various metabolomics applications that involve the use of both gas- and liquid-chromatography.
Subject Keywords
Analytical Chemistry
URI
https://hdl.handle.net/11511/45789
Journal
ANALYTICAL CHEMISTRY
DOI
https://doi.org/10.1021/acs.analchem.6b04690
Collections
Department of Chemistry, Article