New Generation of Solid-Phase Microextraction Coatings for Complementary Separation Approaches: A Step toward Comprehensive Metabolomics and Multiresidue Analyses in Complex Matrices

Download

index.pdf

Date

2017-04-04

Author

Gionfriddo, Emanuela
Boyacı, Ezel
Pawliszyn, Janusz

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

253
views

412
downloads

In this work, a new generation of solid-phase microextraction (SPME) coatings based on polytetrafluoro-ethylene amorphous fluoroplastics (PTFE AF 2400) as a particle binder is presented. The developed coating was tested for thermal and solvent-assisted desorption, demonstrating its compatibility with both gas- and liquid-chromatographic platforms. The incorporation of hydrophilic-lipophilic balance (HLB) adsorptive particles provided optimal extraction coverage for analytes bearing a broad range of hydrophobicities and molecular weights and of varied chemical diversity. The performance of the newly developed coating was compared to already established coatings based on different polymers such as divinylb enzene/carboxen/polydimethylsiloxane (DVB/Car/ PDMS) and octadecyl/benzenesulfonic acid/polyacrylonitrile (C18/SCX/PAN) in order to assess the new prototype versus the existing technology. As this is the first documented instance of PTFE AF being used as a particle immobilizer for SPME, an assessment of the analyte uptake rate and extraction capability of the developed coating was carried out in comparison to other conventionally used polymers. Moreover, the new SPME probes were used to validate an analytical method for deteimination of banned doping substances, achieving limits of quantitation below the minimum required performance limits (MRPLs) set by the World Anti-Doping Agency (WADA) for most compounds. Considering the broad coverage of the coating in terms of analytes extracted and its suitability for both thermal- and solvent assisted desorption, these new SPME probes will properly suit various metabolomics applications that involve the use of both gas- and liquid-chromatography.

Subject Keywords

Analytical Chemistry

URI

https://hdl.handle.net/11511/45789

Journal

ANALYTICAL CHEMISTRY

DOI

https://doi.org/10.1021/acs.analchem.6b04690

Collections

Department of Chemistry, Article

Suggestions

OpenMETU
Core

Immobilization studies utilizing solid supports for the determination of fructose by dansylaminophenyl boronic acid (DAPB acid) and chromate by diphenylcarbazide (DPC) Bulut, Mukadder; Volkan, Mürvet; Department of Chemistry (2006) Immobilization of fluorescent chemosensors and chromogenic reagents on solid supports for developing optical sensors result in improved analytical performance characteristics such as continuous read-out, increased sensitivity, lower reagent consumption and possibility of using the sensor in solvents where the free molecule displays low solubility. The aim of this study is to immobilize dansylaminophenyl boronic acid (DAPB acid) and diphenylcarbazide (DPC) into various solid supports for the determination of...
Use of multi-walled carbon nanotubes in matrix solid phase dispersion extraction combined with gas chromatography Njie, Njaw; Aygün, Rüveyde Sezer; Department of Chemistry (2008) The use of Multi-Walled Carbon Nanotubes (MWCNT) as solid sorbent in Matrix Solid-Phase Dispersion (MSPD) extraction and preconcentration method was presented to determine some commonly used organophosphorus insecticides/OPIs in honey samples using a Gas Chromatography Flame Ionization Detector (GC-FID). OPIs are poisonous compounds used to kill insects and rodents by affecting their nervous system. The limit of detections obtained after MSPD extraction were 7.0 ng/g for Malathion, Malaoxon and Fenitrothion...
On-line preconcentration of vapor forming elements on resistively heated w-coil prior to their determination by atomiz absorption spectrometry Cankur, Oktay; Ataman, Osman Yavuz; Department of Chemistry (2004) Vapor generation in atomic spectrometry is a well established technique for the determination of elements that can be volatilized by chemical reactions. In-situ trapping in graphite furnaces is nowadays one of the most popular methods to increase the sensitivity. In this study, resistively heated W-coil was used as an online trap for preconcentration and revolatilization of volatile species of Bi, Cd and Pb. The collected analyte species were revolatilized rapidly and sent to a quartz Ttube atomizer for AAS...
A Diverse-Stimuli Responsive Chemiluminescent Probe with Luminol Scaffold and Its Electropolymerization Asil Alptekin, Demet; Algi, Fatih; Önal, Ahmet Muhtar (Wiley, 2010-10-01) A diverse-stimuli responsive chemiluminescent system, SNS-Lum, and its electropolymerization to give PSNS-Lum, which is the first example of conjugated polymers with pendant luminol arms inducing chemiluminescence in the presence of super oxide radical anion under neutral conditions, are highlighted.
A bio-sensing platform utilizing a conjugated polymer, carbon nanotubes and PAMAM combination Buber, Ece; Kesik, Melis; Soylemez, Saniye; Toppare, Levent Kamil (Elsevier BV, 2017-08-15) In this study, amperometric biosensing performance of a novel platform utilizing a conducting polymer (CP), multi-walled carbon nanotubes (MWCNTs) and poly(amidoamine) (PAMAM) dendrimer was constructed. In order to fabricate the proposed biosensor, a graphite electrode was modified with poly[9,9-di-(2-ethylhexyl)-fluorenyl-2,7-diyl] end capped with N,N-bis(4-methylphenyl)-4-aniline (PFLA), MWCNTs and PAMAM, respectively. Then, GOx was immobilized onto the modified surface with the help of glutaraldehyde (GA...

Citation Formats

E. Gionfriddo, E. Boyacı, and J. Pawliszyn, “New Generation of Solid-Phase Microextraction Coatings for Complementary Separation Approaches: A Step toward Comprehensive Metabolomics and Multiresidue Analyses in Complex Matrices,” ANALYTICAL CHEMISTRY, pp. 4046–4054, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/45789.