Pullulan microcarriers for bone tissue regeneration

Date

2016-06-01

Author

Aydogdu, Hazal
Keskin, Dilek
Baran, Erkan Turker
Tezcaner, Ayşen

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

142
views

0
downloads

Microcarrier systems offer a convenient way to repair bone defects as injectable cell,carriers that can be applied with small incisions owing to their small size and spherical shape. In this study, pullulan (PULL) microspheres were fabricated and characterized as cell carriers for bone tissue engineering applications. PULL was cross linked by trisodium trimetaphosphate (STMP) to enhance the stability of the microspheres. Improved cytocompatibility was achieved by silk fibroin (SF) coating and biomimetic mineralization on the surface by incubating in simulated body fluid (SBF). X-ray diffraction (XRD), scanning electron microscopy (SEM) and fluorescent microscopy analysis confirmed biomimetic mineralization and SF coating on microspheres. The degradation analysis revealed that PULL microspheres had a slow degradation rate with 8% degradation in two weeks period indicating that the microspheres would support the formation of new bone tissue. Furthermore, the mechanical tests showed that the microspheres had a high mechanical stability that was significantly enhanced with the biomimetic mineralization. In vitro cell culture studies with SaOs-2 cells showed that cell viability was higher on SF and SBF coated microspheres on 7th day compared to PULL ones under dynamic conditions. Alkaline phosphatase activity was higher for SF coated microspheres in comparison to uncoated microspheres when dynamic culture condition was applied. The results suggest that both organic and inorganic surface modifications can be applied on PULL microspheres to prepare a biocompatible microcarrier system with suitable properties for bone tissue engineering.

Subject Keywords

Mechanical Engineering, General Materials Science, Mechanics of Materials, Condensed Matter Physics

URI

https://hdl.handle.net/11511/46046

Journal

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS

DOI

https://doi.org/10.1016/j.msec.2016.03.002

Collections

Department of Engineering Sciences, Article

Suggestions

OpenMETU
Core

Diatom shell incorporated PHBV/PCL-pullulan co-electrospun scaffold for bone tissue engineering Dalgıç, Ali Deniz; Karataş, Ayten; Tezcaner, Ayşen; Keskin, Dilek (Elsevier BV, 2019-07-01) Tissue engineering can benefit from wide variety of materials produced by microorganisms. Natural origin materials often possess good biocompatibility, biodegradability with sustainable production by microorganisms. A phytoplankton, diatom, produces an amorphous silica shell that can be obtained by a cost efficient production process. Diatom shells (DS) are promising for bone tissue engineering since silicon enhances bone regeneration. Biocompatible and biodegradable biopolymers with microorganism origin ca...
Size-independent strength of amorphous-HCP crystalline metallic nanolayers Abboud, Mohammad; Özerinç, Sezer (Cambridge University Press (CUP), 2019-07-15) Amorphous/crystalline (A/C) nanolayers provide an effective model system to study the mechanical behavior and size effects of metallic glasses and crystalline metals in confined geometries. In this work, we experimentally investigated the structure-property relationship in A/C nanolayers containing HCP crystalline layers. CuTi/Ti and CuZr/Zr nanolayers were prepared by magnetron sputtering with layer thicknesses in the range 10-100 nm. The hardness values of the CuTi/Ti and CuZr/Zr nanolayers were close to ...
Investigating the effects of hardening of aluminium alloys on equal-channel angular pressing-A finite-element study Karpuz, P.; Simsir, C.; Gür, Cemil Hakan (Elsevier BV, 2009-03-15) Equal-channel angular pressing (ECAP) is a promising severe plastic deformation method for production of ultrafine-grained bulk metals and alloys with considerably improved mechanical properties. In this study, numerical experiments were carried out to investigate the effect of strain hardening of aluminum alloys on the process performance of ECAP via finite element modeling. In the constitutive model, isothermal-plane strain, frictionless condition was assumed. The numerical results showed that strain hard...
Weight function method for transient thermomechanical fracture analysis of a functionally graded hollow cylinder possessing a circumferential crack Eshraghi, Iman; Soltani, Nasser; Dağ, Serkan (Informa UK Limited, 2016-01-01) This article introduces a weight function method for fracture analysis of a circumferentially cracked functionally graded hollow cylinder subjected to transient thermomechanical loading. Analytical solutions for transient temperature and stress distributions in the uncracked cylinder are derived by applying finite Hankel transformation. These solutions are utilized to determine stress acting on the faces of the circumferential crack in the local perturbation problem. Thermomechanical material properties are...
Efficient fabrication of ultrafine-grained 316L stainless steel surfaces for orthopaedic applications Tufan, Yiğithan; Efe, Mert; Ercan, Batur (Informa UK Limited, 2019-10-13) Commonly used severe plastic deformation (SPD) methods are suitable for fabrication of bulk nano and ultrafine-grained metals. Drawbacks of these methods include durability of dies, geometrical restrictions and reduced ductility of the products. In this study, two common machining techniques used in manufacturing of orthopaedic components, turning and milling, were applied on 316L stainless steel as surface SPD to refine the surface microstructures of the workpiece. Machining with optimised parameters resul...

Citation Formats

H. Aydogdu, D. Keskin, E. T. Baran, and A. Tezcaner, “Pullulan microcarriers for bone tissue regeneration,” MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, pp. 439–449, 2016, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/46046.