Beet molasses-based feeding strategy enhances recombinant thermostable glucose isomerase production by Escherichia coli BL21 (DE3)

Date

2017-11-01

Author

Yaman, Sena
Çalık, Pınar

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The aim of this work was to develop an effective fed-batch feeding strategy to enhance recombinant glucose isomerase (r-GI) production by recombinant Escherichia coli BL21 (DE3) pLysS on an industrially relevant feedstock without the application of an exogenous inducer. Following the batch operation (0 < t < 7H), the effects of pulse and/or continuous feeding of hydrolyzed beet molasses were investigated under five different feeding strategies. The two most promising strategies with respect to r-GI activity were (i) PM-0.05, designed with one pulse feed (t = 7H) followed by a continuous feed and (ii) 2PM(F)-0.05, designed with two consecutive pulse feeds (t = 7 and 10H) followed by a continuous feed. The continuous feeding of molasses for both fermentation strategies employed the same precalculated feeding rate, (o) = 0.05H(-1). The maximum r-GI activities exhibited by PM-0.05 and 2PM(F)-0.05 were 29,050 and 30,642U dm(-3), respectively. On the one hand, compared to PM-0.05 r-GI activity reached its maximum within a shorter cultivation time (t(max) = 2H) at 2PM(F)-0.05, which could be preferable in terms of manufacturing costs and possible risks; on the other hand, PM-0.05 is a simpler fermentation regime compared to 2PM(F)-0.05 with respect to manipulations that should be considered in large-scale production. (C) 2017 International Union of Biochemistry and Molecular Biology, Inc.

Subject Keywords

Biotechnology, Process Chemistry and Technology, Molecular Medicine, Applied Microbiology and Biotechnology, Bioengineering, Drug Discovery, Biomedical Engineering, General Medicine

URI

https://hdl.handle.net/11511/46176

Journal

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

DOI

https://doi.org/10.1002/bab.1549

Collections

Department of Chemical Engineering, Article

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Citation Formats

S. Yaman and P. Çalık, “Beet molasses-based feeding strategy enhances recombinant thermostable glucose isomerase production by Escherichia coli BL21 (DE3),” BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, pp. 944–954, 2017, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/46176.