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Development of a paper-type tyrosinase biosensor for detection of phenolic compounds

2015-01-01
Senyurt, Ozge
EYİDOĞAN, FÜSUN
Yilmaz, Remziye
Oz, M. Tufan
Ozalp, V. Cengiz
Arica, Yakup
Öktem, Hüseyin Avni
A low-cost, portable, and disposable paper-type tyrosinase biosensor was developed for determination of phenolic compounds, using a paper-strip absorption method. Tyrosinase and a chromophore (3-methyl-2-benzothiazolinone hydrazone) were immobilized on paper strips to manufacture the biosensor, which was tested on a nontoxic substrate (l-dopamine). The biosensor was responsive to phenolic compounds such as 4-chlorophenol, catechol, m-cresol, and p-cresol. The sensor showed stability for 70days. The developed biosensor can be used for remote on-site qualitative monitoring of phenolic compounds in wastewater samples.