Development of a paper-type tyrosinase biosensor for detection of phenolic compounds

Date

2015-01-01

Author

Senyurt, Ozge
EYİDOĞAN, FÜSUN
Yilmaz, Remziye
Oz, M. Tufan
Ozalp, V. Cengiz
Arica, Yakup
Öktem, Hüseyin Avni

Metadata

Show full item record

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Item Usage Stats

202
views

0
downloads

A low-cost, portable, and disposable paper-type tyrosinase biosensor was developed for determination of phenolic compounds, using a paper-strip absorption method. Tyrosinase and a chromophore (3-methyl-2-benzothiazolinone hydrazone) were immobilized on paper strips to manufacture the biosensor, which was tested on a nontoxic substrate (l-dopamine). The biosensor was responsive to phenolic compounds such as 4-chlorophenol, catechol, m-cresol, and p-cresol. The sensor showed stability for 70days. The developed biosensor can be used for remote on-site qualitative monitoring of phenolic compounds in wastewater samples.

Subject Keywords

Biotechnology, Process Chemistry and Technology, Molecular Medicine, Applied Microbiology and Biotechnology, Bioengineering, Drug Discovery, Biomedical Engineering, General Medicine

URI

https://hdl.handle.net/11511/46976

Journal

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

DOI

https://doi.org/10.1002/bab.1246

Collections

Department of Biology, Article

Suggestions

OpenMETU
Core

Beet molasses-based feeding strategy enhances recombinant thermostable glucose isomerase production by Escherichia coli BL21 (DE3) Yaman, Sena; Çalık, Pınar (Wiley, 2017-11-01) The aim of this work was to develop an effective fed-batch feeding strategy to enhance recombinant glucose isomerase (r-GI) production by recombinant Escherichia coli BL21 (DE3) pLysS on an industrially relevant feedstock without the application of an exogenous inducer. Following the batch operation (0 < t < 7H), the effects of pulse and/or continuous feeding of hydrolyzed beet molasses were investigated under five different feeding strategies. The two most promising strategies with respect to r-GI activity...
Regulatory effects of alanine-group amino acids on serine alkaline protease production by recombinant Bacillus licheniformis Çalık, Pınar; Ozdamar, TH (Wiley, 2003-04-01) Influences of the concentration and addition time of alanine-group amino acids, i.e. alanine, leucine and valine, on serine alkaline protease (SAP) synthesis were investigated by Bacillus licheniformis (DSM 1969) carrying pHV1431::subC in a defined medium to identify the amino acids creating intracellular reaction-rate limitation in SAP production. While the precursors of alanine-group amino acids, pyruvate and alanine, did not affect SAP production considerably within the range 0-15 mM, the addition of leu...
Development of a synthetic strategy for Water soluble tripodal receptors: Two novel fluorescent receptors for highly selective and sensitive detections of Fe3+ and Cu2+ ions and biological evaluation USLU, AYLİN; Ozcan, Elif; TÜMAY, SÜREYYA OĞUZ; Kazan, Hasan Huseyin; YEŞİLOT, SERKAN (Elsevier BV, 2020-04-01) A general synthetic strategy is developed to synthesize water soluble receptors by employing tripodal system based on a cyclotriphosphaze platform. The developed model is successfully synthesized and characterized by using elemental analysis, FT-IR, MALDI-TOF, H-1 NMR, C-13 NMR and P-31 NMR techniques. The fluorescence sensing performance of prepared water soluble tripodal systems were evaluated by UV/Vis and fluorescence spectroscopies. According to obtained results, two novel water-soluble sensing platfor...
Comparison of anaerobic acrylic acid biotransformation in single- and two-stage pH-stat completely stirred tank reactor systems Demirer, Göksel Niyazi; Speece, RE (Wiley, 2000-01-01) Performance of single- and two-stage pH-stat completely stirred tank reactor (CSTR) systems for anaerobic acrylic acid biotransformation was investigated and compared. In the experiments, acetate was added with acrylic acid. Acetate concentration in both single- and two-stage reactors was continuously maintained at a nonlimiting concentration of 2000 +/- 200 mg/L. using a computer-controlled pH-stat system to allow observation of the effect of acrylate biotransformation on the more sensitive methanogenic st...
The Optimization of Dilute Acid Hydrolysis of Cotton Stalk in Xylose Production AKPINAR, ÖZLEM; Levent, Okan; Bostanci, Seyda; Bakir, Ufuk; Yılmaz, Levent (Springer Science and Business Media LLC, 2011-01-01) Cotton stalk, a lignocellulosic waste material, is composed of xylose that can be used as a raw material for production of xylitol, a high-value product. There is a growing interest in the use of lignocellulosic wastes for conversion into various chemicals because of their low cost and the fact that they are renewable and abundant. The objective of the study was to determine the effects of H(2)SO(4) concentration, temperature, and reaction time on the production of sugars (xylose, glucose, and arabinose) an...

Citation Formats

O. Senyurt et al., “Development of a paper-type tyrosinase biosensor for detection of phenolic compounds,” BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, pp. 132–136, 2015, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/46976.