Beet molasses based exponential feeding strategy for thermostable glucose isomerase production by recombinant Escherichia coli BL21 (DE3)

Date

2013-05-01

Author

Angardi, Vahideh
Çalık, Pınar

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BACKGROUND: The effects of pretreated beet molasses based feeding strategies on thermostable glucose isomerase (GI) production by recombinant Escherichia coli BL21 (DE3) pLysS were investigated. RESULTS: The thermostable GI encoding gene of Thermus thermophilus (xylA) was recombined with pRSETA vector, and the pRSETA::xylA obtained was transferred into E.coli BL21 (DE3) pLysS and used for GI production. The highest soluble GI activity was obtained at t = 30 h, as A = 16 400 U L1 (20.6 U mg1 protein) under molasses based fed-batch operation, with a specific growth rate mu = 0.1 h1 (M-0.1); on the other hand, the highest cell concentration was obtained at mu = 0.15 h1 operation as 9.6 g L1 at t = 32 h. The highest oxygen uptake was 4.57 mol m3 s1 at M-0.1 operation. CONCLUSIONS: Molasses based fed-batch operations were more successful in terms of cell concentration and thermostable enzyme production due to the existence of a natural sugar inducer, galactose, in the molasses composition. This study demonstrates the significance of proper feeding strategy development for over-production of enzymes by recombinant E. coli strains. (c) 2012 Society of Chemical Industry

Subject Keywords

Biotechnology, Fuel Technology, Inorganic Chemistry, Renewable Energy, Sustainability and the Environment, Organic Chemistry, Waste Management and Disposal, Pollution, General Chemical Engineering

URI

https://hdl.handle.net/11511/43289

Journal

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

DOI

https://doi.org/10.1002/jctb.3910

Collections

Department of Chemical Engineering, Article

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Citation Formats

V. Angardi and P. Çalık, “Beet molasses based exponential feeding strategy for thermostable glucose isomerase production by recombinant Escherichia coli BL21 (DE3),” JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, pp. 845–852, 2013, Accessed: 00, 2020. [Online]. Available: https://hdl.handle.net/11511/43289.